2 research outputs found
Pinolenic Acid in Structured Triacylglycerols Exhibits Superior Intestinal Lymphatic Absorption As Compared to Pinolenic Acid in Natural Pine Nut Oil
The
positional distribution pattern of fatty acids (FAs) in the
triacylglycerols (TAGs) affects intestinal absorption of these FAs.
The aim of this study was to compare lymphatic absorption of pinolenic
acid (PLA) present in structured pinolenic TAG (SPT) where PLA was
evenly distributed on the glycerol backbone, with absorption of pine
nut oil (PNO) where PLA was predominantly positioned at the <i>sn</i>-3 position. SPT was prepared via the nonspecific lipase-catalyzed
esterification of glycerol with free FA obtained from PNO. Lymphatic
absorption of PLA from PNO and from SPT was compared in a rat model
of lymphatic cannulation. Significantly (<i>P</i> < 0.05)
greater amounts of PLA were detected in lymph collected for 8 h from
an emulsion containing SPT (28.5 ± 0.7% dose) than from an emulsion
containing PNO (26.2 ± 0.6% dose), thereby indicating that PLA
present in SPT has a greater capacity for lymphatic absorption than
PLA from PNO
Caffeic Acid Phenethyl Ester, a Major Component of Propolis, Suppresses High Fat Diet-Induced Obesity through Inhibiting Adipogenesis at the Mitotic Clonal Expansion Stage
In the present study, we aimed to
investigate the antiobesity effect
of CAPE in vivo, and the mechanism by which CAPE regulates body weight
in vitro. To confirm the antiobesity effect of CAPE in vivo, mice
were fed with a high fat diet (HFD) with different concentrations
of CAPE for 5 weeks. CAPE significantly reduced body weight gain and
epididymal fat mass in obese mice fed a HFD. In accordance with in
vivo results, Oil red O staining results showed that CAPE significantly
suppressed MDI-induced adipogenesis of 3T3-L1 preadipocytes. FACS
analysis results showed that CAPE delayed MDI-stimulated cell cycle
progression, thereby contributing to inhibit mitotic clonal expansion
(MCE), which is a prerequisite step for adipogenesis. Also, CAPE regulated
the expression of cyclin D1 and the phosphorylation of ERK and Akt,
which are upstream of cyclin D1. These results suggest that CAPE exerts
an antiobesity effect in vivo, presumably through inhibiting adipogenesis
at an early stage of adipogenesis