Caffeic
Acid Phenethyl Ester, a Major Component of
Propolis, Suppresses High Fat Diet-Induced Obesity through Inhibiting
Adipogenesis at the Mitotic Clonal Expansion Stage
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Abstract
In the present study, we aimed to
investigate the antiobesity effect
of CAPE in vivo, and the mechanism by which CAPE regulates body weight
in vitro. To confirm the antiobesity effect of CAPE in vivo, mice
were fed with a high fat diet (HFD) with different concentrations
of CAPE for 5 weeks. CAPE significantly reduced body weight gain and
epididymal fat mass in obese mice fed a HFD. In accordance with in
vivo results, Oil red O staining results showed that CAPE significantly
suppressed MDI-induced adipogenesis of 3T3-L1 preadipocytes. FACS
analysis results showed that CAPE delayed MDI-stimulated cell cycle
progression, thereby contributing to inhibit mitotic clonal expansion
(MCE), which is a prerequisite step for adipogenesis. Also, CAPE regulated
the expression of cyclin D1 and the phosphorylation of ERK and Akt,
which are upstream of cyclin D1. These results suggest that CAPE exerts
an antiobesity effect in vivo, presumably through inhibiting adipogenesis
at an early stage of adipogenesis