Artemisinin Story from the Balkans

The isolation is reported of artemisinin (qinghaosu), a drug remarkably effective against malaria, from the aerial parts of Artemisia annua L. (sweet wormwood) at the Department of Chemistry, University of Belgrade (now Faculty of Chemistry), Serbia by the end of 1970, almost two years before the isolation of the same compound in China

Sekundarni metaboliti biljne vrste Hypericum monogynum iz Pakistana

4-Chlorobenzoic acid (1), quercitrin (2), astilbin (3), along with beta-sitosterol, gamma-sitosterol, friedelin and beta-amyrin were isolated from the aerial parts of Hypericum monogynum. Whereas compound 1 was isolated for the first time from natural sources, flavanonol 3 was not found before in these species.4-Hlorobenzoeva kiselina (1), kvercitrin (2), astilbin (3), kao i β-sitosterol, γ- sitosterol, fridelin i β-amirin izolovani su iz nadzemnih delova biljne vrste Hypericum monogynum. Po prvi put jedinjenje 1 je izolovano iz prirodnih izvora, dok flavanonol 3 do sada nije izolovan iz ove biljne vrste

Hemijski sastav ekstrakta semenki bele ribizle

From the seeds of white currant (Ribes rubrum, cv. White Champagne), a new sesquiterpenoid glycoside 1 was isolated, along with two known compounds: dihydrophaseic acid 3'-O-beta-D-glucopyranoside (2), and 3-carboxymethyl-indole-1-N-beta-D-glucopyranoside (3). The structure of the new compound was identified as dihydrophaseic acid 3'-O-beta-gentiobioside, based on extensive NMR and MS spectral studies.Iz semena bele ribizle (Ribes rubrum, cv. bela šampanjska) izolovan je novi seskviterpenski glukozid (1), zajedno sa dva poznata jedinjenja: 3'-O-b-D-glukopiranozidom dihidrofazeinske kiseline (2) i 3-karboksimetilindol-1-N-b-D-glukopiranozidom (3). Na osnovu detaljnih NMR i MS studija, struktura novog jedinjenja je određena kao 3'-O-b-genciobiozid dihidrofazeinske kiseline

The role of a pharmacist in prevention and detection of OTC drug interactions: Nominal group technique

Introduction: Nowadays number of newly approved OTC medicines continuously increases, which can make certain difficulties in drug dispensing process. This fact particularly relates to drug-drug interactions-problems due to lack of data and insufficient of knowledge about the interactions. Aim: To identify the problems pharmacists face when dispensing OTC drugs and to recommend concrete ideas and methods for improving system for detecting OTC drugs interactions. Participants and method: The study was carried out in accordance with methodological principles of nominal group technique. Two meetings of nominal group were conducted, enrolling pharmacists employed in public pharmacies. During the meetings, moderator imposed aset of questions to participants, who gave answers in the form of individual statements. In order to examine agreement of the participants about the individual statements, at the end of both sessions the statements were rated on a Likert's scale. Meetings were recorded and audio recordings later used for qualitative analysis. Results: Nine pharmacists of both sex (7/2 females/males) participated in this study. Average age of the participants was 25.0±1.0 years. During nominal group meetings, participants presented 30 statements on 7 research questions, and after the second meeting agreement was reached about 29 of them. Qualitative analysis of data indicated three categories of statements: (1) pharmacists' knowledge of OTC drugs interactions, (2) problems pharmacists face when dispensing OTC drugs and (3) options of improving system for prevention and detection of OTC drugs interactions. Conclusion: For adequate prevention of potential OTC drugs interactions it is necessary to make guidelines with recommendations referring to proper dispensing procedure and to use regularly available online interaction checkers

Koncentracija trans- i cis-rezveratrola u vinima proizvedenim u Srbiji

Resveratrol, which occurs in two isomeric forms, trans and cis, is a phytoalexin with numerous pharmacological activities, such as anti-cancer, antiviral, neuroprotective and anti-aging. Red wine is the main source of the compound and an easy way of including resveratrol in the human diet. In this study, the most popular commercial Serbian wines (red, white and rose-type) were analyzed for their content of trans- and cis-resveratrol. The analysis was performed by HPLC with a UV detector. Prior to the injection, phenolic compounds were extracted onto a LiChrolut RP18 bonded silica cartridge. The concentration of trans-resveratrol ranged from 0.11 to 1.69 mg L-1 and cis-resveratrol from 0.12 to 1.49 mg L-1.Metodom tečne hromatografije (HPLC) ispitan je sadržaj slobodnog oblika trans- i cis-rezveratrola u osamnaest komercijalnih uzoraka (10 crvenih, 7 belih i 1 roze) srpskih vina. Svi uzorci su pre hromatografije ekstrahovani SPE tehnikom na LiChrolut RP18 koloni. trans-Rezveratrol je detektovan u 17 od 18 analiziranih uzoraka vina sa prosečnom koncentracijama od 0,78 mg l-1 za crvena vina i 0,23 mg l-1 za bela vina. Najviša koncentracija trans-rezveratrola je nađena u uzorku crvenog vina Cabernet Sauvignon berbe 2002 godine. cis-Rezveratrol je detektovan u 12 od 18 analiziranih uzoraka vina sa prosečnom koncentracijom 0,55 mg l-1 za crvena vina, dok je u belim vinima od analiziranih 7 uzoraka detektovan samo u 2 uzorka sa koncentracijama 0,12 i 0,49 mg l-1. Visok sadržaj cis-rezveratrola u nekim uzorcima je verovatno posledica izomerizacije trans- u cis-rezveratrol tokom procesa proizvodnje vina

Supplementary material for the article: Cvetković, M.; Damjanović, A.; Stanojković, T. P.; Đorđević, I.; Tešević, V.; Milosavljević, S.; Gođevac, D. Integration of Dry-Column Flash Chromatography with NMR and FTIR Metabolomics to Reveal Cytotoxic Metabolites from Amphoricarpos Autariatus. Talanta 2020, 206. https://doi.org/10.1016/j.talanta.2019.120248

Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3348]Supplementary material for: [https://www.sciencedirect.com/science/article/pii/S0039914019308811?via%3Dihub

Volatile Constituents of Different Plant Parts and Populations of Malabaila aurea Boiss. from Montenegro

The volatile constituents of different plant parts and populations of Malabaila aurea Boiss. from Montenegro were obtained by simultaneous distillation-extraction and analyzed by GC-FID and GC-MS. A total of 12 samples were examined and 45 compounds were identified. The volatile content of different M. aurea populations was very similar, while the volatile fractions obtained from different plant parts showed significant qualitative and quantitative differences. The most abundant compounds found in stems & leaves were apiole (51.0-56.3%), myristicin (16.3-25.4%), and falcarinol (4.1-10.7%). The roots showed the same major components, but with different relative abundances: 30.9-49.1% of apiole, 12.9-34.7% of falcarinol, and 9.9-31.1% of myristicin. The volatile constituents of fruits & flowers were remarkably different, containing up to 71.2-80.5% octyl butyrate, 11.4-18.0% octanol, and 2.7-6.8% octyl hexanoate. The results were discussed as possible indication of relatedness of Malabaila aurea and Pastinaca sativa (parsnip)

Hemijski sastav i antifungalna aktivnost etarskog ulja daglasove jele (Pseudosuga menziesii mirb. Franco) iz Srbije

The chemical composition of the essential oil of fresh young needles with twigs of Douglas fir (Pseudosuga menziesii Mirb. Franco) obtained by hydrodistillation were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Ten compounds, accounting for 94.26 % of the oil, were identified. The main compounds found were bornyl acetate (34.65 %), camphene (29.82 %), alpha-pinene (11.65 %) and santene (5.45 %). The antifiangal activity of the essential oil was tested against various fungal species. The minimum inhibitory concentration of Douglas fir essential oil ranged from 1.5 to 4 mu g mL(-1). The fungi most sensitive to the tested oil were Phomopsis helianthi, while Penicillium species, along with Microsporum canis, were the most resistant. Compared to the commercial fungicidal agent bifonazole, the studied essential oil demonstrated higher antifungal activity.Hemijski sastav etarskog ulja mladih iglica sa grančicama duglazije (Pseudosuga menziesii Mirb. Franco) dobijenog destilacijom vodenom parom, analiziran je gasnom hromatografijom (GC) i kombinacijom gasne hromatografije i masene spektrometrije (GC/MS). Identifikovano je deset jedinjenja ukupne zastupljenosti 94,26 %. Kao glavne komponente su nađeni: bornil-acetat (34,65 %), kamfen (29,82 %), α-pinen (11,65 %) i santen (5,45 %). Pored toga, etarsko ulje je testirano na antifungalnu aktivnost. Etarsko ulje pokazuje mnogo bolju antifugalnu akivnost od komercijalnog fungicidnog agensa bifonazola. Minimalna inhibitorna koncentracija etarskog ulja duglazije je u opsegu od 0,6 do 1,4 μL mL-1. Najosetljiviji na testirano ulje je bio soj Phomopsis helianthi, dok sojevi Penicillium i Microsporum canis pokazuju najveću rezistentnost

Flavonoidi iz nadzemnih delova Onobrychis montana subsp. scardica

Rutin (1, main constituent) and two flavone C-glycosides, vitexin (2) and vitexin 2 ''-O-alpha-rhamnopyranoside (3) were isolated from the aerial parts of Onobrychis montana subsp. scardica. They were identified by (1)H-NMR, (13)C-NMR and UV-Vis spectroscopy (procedure with shift reagents), and high resolution ESI-MS. A relatively high content of 1 (5.27 mg/g of dry plant material), measured by HPLC, indicated O. montana subsp. scardica as a new natural source of this biologically active compound. The isolated flavonoid compounds might be of value as chemotaxonomic markers.Iz nadzemnih delova Onobrychis montana subsp. scardica izolovani su rutin (1, glavni sastojak) i dva flavonoidna S-glikozida, viteksin (2) i njegov 2''-O-α-ramnopiranozid (3). Njihova struktura je određena primenom 1H-NMR, 13C-NMR i UV spektroskopije (procedura sa reagensima UV-pomeranja) i masene spektrometrije visokog razlaganja (HR-ESI-MS). Relativno visok sadržaj rutina (5.27 mg/g suvog materijala) određen pomoću tečne hromatografije (HPLC), ukazuje na O. montana subsp. scardica kao nov prirodni izvor ovog biološki aktivnog jedinjenja. Izolovana jedinjenja mogu biti i od vrednosti kao hemotaksonomski markeri

Supplementary material for the article: Cvetković, M.; Damjanović, A.; Stanojković, T. P.; Đorđević, I.; Tešević, V.; Milosavljević, S.; Gođevac, D. Integration of Dry-Column Flash Chromatography with NMR and FTIR Metabolomics to Reveal Cytotoxic Metabolites from Amphoricarpos Autariatus. Talanta 2020, 206. https://doi.org/10.1016/j.talanta.2019.120248

Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3348]Supplementary material for: [https://www.sciencedirect.com/science/article/pii/S0039914019308811?via%3Dihub