Antioxidative and antibacterial activities of phenolic compounds from Ficus sur Forssk. and Ficus sycomorus L. (Moraceae) : potential for sickle cell disease treatment in Burkina Faso

Ficus sur and Ficus sycomorus, two medicinal species used in Burkinabe traditional medicine for the treatment of sickle cell disease were investigated. The determination of total phenolic and tannins contents, using the method of Folin-Ciocalteu (FCR), indicate values significantly (p <0.05) higher in extracts of Ficus sycomorus compared to those of Ficus sur, with respective values of 336.80 ± 0.80 mg TAE/g of extract and 203.74 ± 0.9 mg TAE/g of extract against 247.00 ± 0.52 mg TAE/g of extract and 120.8 ± 0.83 mg TAE/g of extract. Similarly, using the method of 1,1-diphenyl-2-picrylhydrazyl (DPPH), the results of the analysis indicate that extracts of Ficus sycomorus produce the highest antiradical activity with IC50 value of 9.60 ± 0.02 μg/mL against 31.83 ± 0.55 μg/ mL for Ficus sur. The IC50 value of quercetin, used as reference antioxidant, was of 4.6 ± 0.08 μg/mL. The latex of Ficus sycomorus presented the lowest minimum inhibitory concentrations (MIC) against Staphylococcus aureus and Escherichia coli, with respective values of 0.13 mg/mL and 0.25 mg/mL. The difference in phenolic content could explain the difference in biological activity between the two Ficus species.Keywords: drepanocitosis, radical scavenging, tannins, flavonoids, medicinal plant

Study of the antibacterial activity of the stem bark and leaf extracts of Parkia biglobosa (Jacq.) Benth. on Satphylococcus aureus

The antibacterial activity of the stem bark and the leaves of Parkia biglobosa have been tested on four strains of Staphylococcus aureus isolated from patients in the National Hospital Yalgado Ouedraogo – Ouagadougou, Burkina Faso. The tests revealed the efficacy of the hydroalcoholic and the aqueous extracts. They also revealed that the hydroalcoholic extracts were more active than the aqueous extract prescribed by the traditional healers and that the hydroalcoholic extract of the leaves were as active as the bark. The effects of the extracts have been compared to that of the gentamicin, a positive control. The phytochemical screening showed that the extracts of the stem bark were rich in sterols, a triterpenes, tannins, saponosides, anthocyanidins, coumarins, flavonoids and reducing coumpounds. The leaves extracts were rich in tannins, coumarins, anthocyanins, flavones and reducing compounds. Keywords: Staphylococcus aureus, Parkia biglobosa, antibacterial activity African Journal of Traditional, Complementary and Alternative Medicines Vol. 3(2) 2006: 74-7

Antimicrobial Effects of the Stem Bark Extracts of Parkia Biglobosa (Jacq.) Benth. on Shigellae

Total and hydroalcoholic extracts of the stem barks of Parkia biglobosa (Jacq) Benth. (Mimosaceae) were tested on strains belonging to three species of Shigellae: S. dysenteriae, S. flexneri and S. boydii collected from hospitals in Ouagadougou, Burkina Faso. The results showed that both extracts were active against Shigellae. The hydroalcoholic extract was more active than the decoction (aqueous one) prescribed by the traditional healer. Both extracts were particularly effective against S. dysenteriae, the most virulent of the three pathogenic species. The effects of the extracts have been compared to that of gentamicin. The phytochemical screening on the extracts revealed the presence of sterols, triterpenes, polyphenolic compounds including tanins, flavonoids, coumarins, anthocyanidins. Other components are saponosides and reducing sugars

Comparative Study of Leaf and Stem Bark Extracts of Parkia Biglobosa Against Enterobacteria

Hydroethanolic and aqueous extracts of leaf and stem bark of Parkia biglobosa (Jacq) Benth. (Mimosaceae) were tested against clinical isolates Escherichia coli, Salmonella typhi, Shigella dysenteriae and Enterococcus faecalis, and corresponding collection strains E. coli CIP 105 182, Salmonella enterica CIP 105 150, Shigella dysenteriae CIP 54-51 and Enterococcus faecalis CIP 103 907. Discs of Gentamicin, a broad spectrum antibiotic were used as positive controls. The results showed that all the extracts possess antimicrobial activities. A comparative study of the antibacterial activity of the leaves and that of the bark showed that for all the tested microorganisms, the hydroalcoholic extract of the bark is more active than the aqueous extract of the leaf. The hydroethanolic extract of the leaves is as effective as the aqueous extract of the stem bark prescribed by the traditional healer, suggesting it is possible to use leaves other than the roots and bark. The phytochemical screening showed that sterols and triterpenes, saponosides, tannins, reducing compounds, coumarins, anthocyanosides, flavonosides are present in both bark and leaf but in different concentrations

STUDY OF THE ANTIBACTERIAL ACTIVITY OF THE STEM BARK AND LEAF EXTRACTS OF PARKIA BIGLOBOSA (JACQ.) BENTH. ON SATPHYLOCOCCUS AUREUS

The antibacterial activity of the stem bark and the leaves of Parkia biglobosa have been tested on four strains of Staphylococcus aureus isolated from patients in the National Hospital Yalgado Ouedraogo – Ouagadougou, Burkina Faso. The tests revealed the efficacy of the hydroalcoholic and the aqueous extracts. They also revealed that the hydroalcoholic extracts were more active than the aqueous extract prescribed by the traditional healers and that the hydroalcoholic extract of the leaves were as active as the bark. The effects of the extracts have been compared to that of the gentamicin, a positive control. The phytochemical screening showed that the extracts of the stem bark were rich in sterols, a triterpenes, tannins, saponosides, anthocyanidins, coumarins, flavonoids and reducing coumpounds. The leaves extracts were rich in tannins, coumarins, anthocyanins, flavones and reducing compounds