Internship Preparedness: A Look into the Internship Experience at St. John Fisher College

In the field of sport management, internships have been shown to be an important factor in the initial placement in the sport industry. Previous work has cited both design elements and the experience itself as being critical to internship success or failure. As the SJFC program requires two distinct required experiences, the purpose of this study was to explore the impact of these experiences on recent graduate’s confidence levels. Through survey data it was uncovered that participants who indicated a higher satisfaction with their final internship were more likely to end up in a career similar that of their last internship. Additionally, qualitative and quantitative data highlighted the design factors, such as learning opportunities and task variety as a very important factor in determining internship effectiveness. Qualitative data confirmed that many different aspects played a role in the effectiveness of the internship, but what it came down to was the experience itself. Sport management program faculty, current and potential students, and internship supervisors would find this information useful due to the insight this study provides

Decay Phase Cooling and Inferred Heating of M- and X-class Solar Flares

In this paper, the cooling of 72 M- and X-class flares is examined using GOES/XRS and SDO/EVE. The observed cooling rates are quantified and the observed total cooling times are compared to the predictions of an analytical 0-D hydrodynamic model. It is found that the model does not fit the observations well, but does provide a well defined lower limit on a flare's total cooling time. The discrepancy between observations and the model is then assumed to be primarily due to heating during the decay phase. The decay phase heating necessary to account for the discrepancy is quantified and found be ~50% of the total thermally radiated energy as calculated with GOES. This decay phase heating is found to scale with the observed peak thermal energy. It is predicted that approximating the total thermal energy from the peak is minimally affected by the decay phase heating in small flares. However, in the most energetic flares the decay phase heating inferred from the model can be several times greater than the peak thermal energy.Comment: Published in the Astrophysical Journal, 201

Evidence of a Plasmoid-Looptop Interaction and Magnetic Inflows During a Solar Flare/CME Eruptive Event

Observational evidence is presented for the merging of a downward-propagating plasmoid with a looptop kernel during an occulted limb event on 2007 January 25. RHESSI lightcurves in the 9-18 keV energy range, as well as that of the 245 MHz channel of the Learmonth Solar Observatory, show enhanced nonthermal emission in the corona at the time of the merging suggesting that additional particle acceleration took place. This was attributed to a secondary episode of reconnection in the current sheet that formed between the two merging sources. RHESSI images were used to establish a mean downward velocity of the plasmoid of 12 km/s. Complementary observations from the SECCHI suite of instruments onboard STEREO-Behind showed that this process occurred during the acceleration phase of the associated CME. From wavelet-enhanced EUVI, images evidence of inflowing magnetic field lines prior to the CME eruption is also presented. The derived inflow velocity was found to be 1.5 km/s. This combination of observations supports a recent numerical simulation of plasmoid formation, propagation and subsequent particle acceleration due to the tearing mode instability during current sheet formation.Comment: 8 pages, 9 figures, ApJ (Accepted

A single amino acid determines preference between phospholipids and reveals length restriction for activation ofthe S1P₄ receptor

Background<br/><br/> Sphingosine-1-phosphate and lysophosphatidic acid (LPA) are ligands for two related families of G protein-coupled receptors, the S1P and LPA receptors, respectively. The lysophospholipid ligands of these receptors are structurally similar, however recognition of these lipids by these receptors is highly selective. A single residue present within the third transmembrane domain (TM) of S1P receptors is thought to determine ligand selectivity; replacement of the naturally occurring glutamic acid with glutamine (present at this position in the LPA receptors) has previously been shown to be sufficient to change the specificity of S1P<sub>1</sub> from S1P to 18:1 LPA.<br/><br/> Results<br/><br/> We tested whether mutation of this "ligand selectivity" residue to glutamine could confer LPA-responsiveness to the related S1P receptor, S1P<sub>4</sub>. This mutation severely affected the response of S1P<sub>4</sub> to S1P in a [<sup>35</sup>S]GTPγS binding assay, and imparted sensitivity to LPA species in the order 14:0 LPA > 16:0 LPA > 18:1 LPA. These results indicate a length restriction for activation of this receptor and demonstrate the utility of using LPA-responsive S1P receptor mutants to probe binding pocket length using readily available LPA species. Computational modelling of the interactions between these ligands and both wild type and mutant S1P<sub>4</sub> receptors showed excellent agreement with experimental data, therefore confirming the fundamental role of this residue in ligand recognition by S1P receptors.<br/><br/> Conclusions<br/><br/> Glutamic acid in the third transmembrane domain of the S1P receptors is a general selectivity switch regulating response to S1P over the closely related phospholipids, LPA. Mutation of this residue to glutamine confers LPA responsiveness with preference for short-chain species. The preference for short-chain LPA species indicates a length restriction different from the closely related S1P<sub>1</sub> receptor

Performance of Major Flare Watches from the Max Millennium Program (2001-2010)

The physical processes that trigger solar flares are not well understood and significant debate remains around processes governing particle acceleration, energy partition, and particle and energy transport. Observations at high resolution in energy, time, and space are required in multiple energy ranges over the whole course of many flares in order to build an understanding of these processes. Obtaining high-quality, co-temporal data from ground- and space- based instruments is crucial to achieving this goal and was the primary motivation for starting the Max Millennium program and Major Flare Watch (MFW) alerts, aimed at coordinating observations of all flares >X1 GOES X-ray classification (including those partially occulted by the limb). We present a review of the performance of MFWs from 1 February 2001 to 31 May 2010, inclusive, that finds: (1) 220 MFWs were issued in 3,407 days considered (6.5% duty cycle), with these occurring in 32 uninterrupted periods that typically last 2-8 days; (2) 56% of flares >X1 were caught, occurring in 19% of MFW days; (3) MFW periods ended at suitable times, but substantial gain could have been achieved in percentage of flares caught if periods had started 24 h earlier; (4) MFWs successfully forecast X-class flares with a true skill statistic (TSS) verification metric score of 0.500, that is comparable to a categorical flare/no-flare interpretation of the NOAA Space Weather Prediction Centre probabilistic forecasts (TSS = 0.488).Comment: 19 pages, 2 figures, accepted for publication in Solar Physic

Chemically engineering ligand selectivity at the free fatty acid receptor 2 based on pharmacological variation between species orthologs

When it is difficult to develop selective ligands within a family of related G-protein-coupled receptors (GPCRs), chemically engineered receptors activated solely by synthetic ligands (RASSLs) are useful alternatives for probing receptor function. In the present work, we explored whether a RASSL of the free fatty acid receptor 2 (FFA2) could be developed on the basis of pharmacological variation between species orthologs. For this, bovine FFA2 was characterized, revealing distinct ligand selectivity compared with human FFA2. Homology modeling and mutational analysis demonstrated a single mutation in human FFA2 of C4.57G resulted in a human FFA2 receptor with ligand selectivity similar to the bovine receptor. This was exploited to generate human FFA2-RASSL by the addition of a second mutation at a known orthosteric ligand interaction site, H6.55Q. The resulting FFA2-RASSL displayed a >100-fold loss of activity to endogenous ligands, while responding to the distinct ligand sorbic acid with pEC(50) values for inhibition of cAMP, 5.83 ± 0.11; Ca(2+) mobilization, 4.63 ± 0.05; ERK phosphorylation, 5.61 ± 0.06; and dynamic mass redistribution, 5.35 ± 0.06. This FFA2-RASSL will be useful in future studies on this receptor and demonstrates that exploitation of pharmacological variation between species orthologs is a powerful method to generate novel chemically engineered GPCRs

RHESSI and SOHO/CDS Observations of Explosive Chromospheric Evaporation

Simultaneous observations of explosive chromospheric evaporation are presented using data from the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) and the Coronal Diagnostic Spectrometer (CDS) onboard SOHO. For the first time, co-spatial imaging and spectroscopy have been used to observe explosive evaporation within a hard X-ray emitting region. RHESSI X-ray images and spectra were used to determine the flux of non-thermal electrons accelerated during the impulsive phase of an M2.2 flare. Assuming a thick-target model, the injected electron spectrum was found to have a spectral index of ~7.3, a low energy cut-off of ~20 keV, and a resulting flux of >4x10^10 ergs cm^-2 s^-1. The dynamic response of the atmosphere was determined using CDS spectra, finding a mean upflow velocity of 230+/-38 km s^-1 in Fe XIX (592.23A), and associated downflows of 36+/-16 km s^-1 and 43+/-22 km s^-1 at chromospheric and transition region temperatures, respectively, relative to an averaged quiet-Sun spectra. The errors represent a 1 sigma dispersion. The properties of the accelerated electron spectrum and the corresponding evaporative velocities were found to be consistent with the predictions of theory.Comment: 5 pages, 4 figures, ApJL (In Press

Multi-wavelength observations and modelling of a canonical solar flare

This paper investigates the temporal evolution of temperature, emission measure, energy loss and velocity in a C-class solar flare from both an observational and theoretical perspective. The properties of the flare were derived by following the systematic cooling of the plasma through the response functions of a number of instruments -- RHESSI (>5 MK), GOES-12 (5-30 MK), TRACE 171 A (1 MK) and SOHO/CDS (~0.03-8 MK). These measurements were studied in combination with simulations from the 0-D EBTEL model. At the flare on-set, upflows of ~90 km s-1 and low level emission were observed in Fe XIX, consistent with pre-flare heating and gentle chromospheric evaporation. During the impulsive phase, upflows of ~80 km s-1 in Fe XIX and simultaneous downflows of 20 km s-1 in He I and O V were observed, indicating explosive chromospheric evaporation. The plasma was subsequently found to reach a peak temperature of ~13 MK in approximately 10 minutes. Using EBTEL, conduction was found to be the dominant loss mechanism during the initial ~300s of the decay phase. It was also found to be responsible for driving gentle chromospheric evaporation during this period. As the temperature fell below ~8 MK, and for the next ~4,000s, radiative losses were determined to dominate over conductive losses. The radiative loss phase was accompanied by significant downflows of <40 km s-1 in O V. This is the first extensive study of the evolution of a canonical solar flare using both spectroscopic and broad-band instruments in conjunction with a hydrodynamic model. While our results are in broad agreement with the standard flare model, the simulations suggest that both conductive and non-thermal beam heating play important roles in heating the flare plasma during the impulsive phase of at least this event.Comment: 10 pages, 7 figures, 2 tables. Accepted for publication in A&