A Better Definition of the Kilogram

This article reviews several recent proposed redefinitions of the kilogram, and compares them with respect to practical realizations, uncertainties (estimated standard deviations), and educational aspects.Comment: 10 pages, no figure

MicroRNA profiling reveals marker of motor neuron disease in ALS models

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder marked by the loss of motor neurons (MNs) in the brain and spinal cord, leading to fatally debilitating weakness. Because this disease predominantly affects MNs, we aimed to characterize the distinct expression profile of that cell type to elucidate underlying disease mechanisms and to identify novel targets that inform on MN health during ALS disease time course. microRNAs (miRNAs) are short, noncoding RNAs that can shape the expression profile of a cell and thus often exhibit cell-type-enriched expression. To determine MN-enriched miRNA expression, we used Cre recombinase-dependent miRNA tagging and affinity purification in mice. By defining thein vivomiRNA expression of MNs, all neurons, astrocytes, and microglia, we then focused on MN-enriched miRNAs via a comparative analysis and found that they may functionally distinguish MNs postnatally from other spinal neurons. Characterizing the levels of the MN-enriched miRNAs in CSF harvested from ALS models of MN disease demonstrated that one miRNA (miR-218) tracked with MN loss and was responsive to an ALS therapy in rodent models. Therefore, we have used cellular expression profiling tools to define the distinct miRNA expression of MNs, which is likely to enrich future studies of MN disease. This approach enabled the development of a novel, drug-responsive marker of MN disease in ALS rodents.SIGNIFICANCE STATEMENTAmyotrophic lateral sclerosis (ALS) is a neurodegenerative disease in which motor neurons (MNs) in the brain and spinal cord are selectively lost. To develop tools to aid in our understanding of the distinct expression profiles of MNs and, ultimately, to monitor MN disease progression, we identified small regulatory microRNAs (miRNAs) that were highly enriched or exclusive in MNs. The signal for one of these MN-enriched miRNAs is detectable in spinal tap biofluid from an ALS rat model, where its levels change as disease progresses, suggesting that it may be a clinically useful marker of disease status. Furthermore, rats treated with ALS therapy have restored expression of this MN RNA marker, making it an MN-specific and drug-responsive marker for ALS rodents.</jats:p

LIMITED ANTIBODY EVIDENCE OF EXPOSURE TO MYCOBACTERIUM BOVIS IN FERAL SWINE (\u3ci\u3eSUS SCROFA\u3c/i\u3e) IN THE USA

Bovine tuberculosis is a chronic disease of cattle (Bos taurus) caused by the bacterium Mycobacterium bovis. Efforts have been made in the US to eradicate the disease in cattle, but spillover into wildlife and subsequent spillback have impeded progress in some states. In particular, infection in white-tailed deer (Odocoileus virginianus) has been followed by infection in cattle in some Midwestern states. Infection has also been documented in feral swine (Sus scrofa) on the Hawaiian island of Molokai and in various European countries, but no large-scale survey of antibody exposure to the bacteria has been conducted in feral swine in the US. We tested 488 sera from feral swine collected near previously documented outbreaks of bovine tuberculosis in cattle and captive cervids, in addition to 2,237 feral swine sera collected across the US from 1 October 2013 to 30 September 2014. While all but one of the samples were antibody negative, the results are important for establishing baseline negative data since feral swine are capable reservoirs and could be implicated in future outbreaks of the disease

Observations of the Crab Nebula and its pulsar in the far-ultraviolet and in the optical

We present HST/STIS far-UV observations of the Crab nebula and its pulsar. Broad, blueshifted absorption arising in the nebula is seen in C IV 1550, reaching about 2500 km/s. This can be interpreted as evidence for a fast outer shell, and we adopt a spherically symmetric model to constrain the properties of this. We find that the density appears to decrease outward in the shell. A lower limit to the mass is 0.3 solar masses with an accompanying kinetic energy of 1.5EE{49} ergs. A massive 10^{51} erg shell cannot be excluded, but is less likely if the density profile is much steeper than R^{-4} and the velocity is <6000 km/s. The observations cover the region 1140-1720 A. With the time-tag mode of the spectrograph we obtain the pulse profile. It is similar to that in the near-UV, although the primary peak is marginally narrower. Together with the near-UV data, and new optical data from NOT, our spectrum of the pulsar covers the entire region from 1140-9250 A. Dereddening the spectrum gives a flat spectrum for E(B-V)=0.52, R=3.1. This dereddened spectrum of the Crab pulsar can be fitted by a power law with spectral index alpha_{\nu} = 0.11 +/- 0.04. The main uncertainty is the amount and characteristics of the interstel- lar reddening, and we have investigated the dependence of \alpha_{\nu} on E(B-V) and R. In the extended emission covered by our 25" x 0.5" slit in the far-UV, we detect C IV 1550 and He II 1640 emission lines from the Crab nebula. Several interstellar absorption lines are detected toward the pulsar. The Ly alpha absorption indicates a column density of 3.0+/-0.5\EE{21} cm^{-2} of neutral hydrogen, which agrees well with our estimate of E(B-V)=0.52 mag. Other lines show no evidence of severe depletion of metals in atomic gas.Comment: 18 pages emulateapj style, including 10 figures. ApJ, accepte

Software systems for operation, control, and monitoring of the EBEX instrument

We present the hardware and software systems implementing autonomous operation, distributed real-time monitoring, and control for the EBEX instrument. EBEX is a NASA-funded balloon-borne microwave polarimeter designed for a 14 day Antarctic flight that circumnavigates the pole. To meet its science goals the EBEX instrument autonomously executes several tasks in parallel: it collects attitude data and maintains pointing control in order to adhere to an observing schedule; tunes and operates up to 1920 TES bolometers and 120 SQUID amplifiers controlled by as many as 30 embedded computers; coordinates and dispatches jobs across an onboard computer network to manage this detector readout system; logs over 3~GiB/hour of science and housekeeping data to an onboard disk storage array; responds to a variety of commands and exogenous events; and downlinks multiple heterogeneous data streams representing a selected subset of the total logged data. Most of the systems implementing these functions have been tested during a recent engineering flight of the payload, and have proven to meet the target requirements. The EBEX ground segment couples uplink and downlink hardware to a client-server software stack, enabling real-time monitoring and command responsibility to be distributed across the public internet or other standard computer networks. Using the emerging dirfile standard as a uniform intermediate data format, a variety of front end programs provide access to different components and views of the downlinked data products. This distributed architecture was demonstrated operating across multiple widely dispersed sites prior to and during the EBEX engineering flight.Comment: 11 pages, to appear in Proceedings of SPIE Astronomical Telescopes and Instrumentation 2010; adjusted metadata for arXiv submissio