9 research outputs found
Biochemical indicators of sports achievements in highly qualified wrestlers
Objective: to conduct comparative analysis of blood biochemical parameters (urea, ALT, AST, creatinkinase, cortisol, testosterone and testosterone / cortisol ratio) in groups of highΒclass wrestlers with different levels of sports achievements.Materials and methods: male athletes (n = 78), members of the Russian national team in one of the types of wrestling (the average age is 25.2 (21.5β28.9) years, the average weight is 76.9 (68.4β83.4) kg) were recruited to this study. The examined athletes were divided into two groups according to their sporting achievements. The first one β SHA group (superΒhigh achievements) which included athletes (n = 19) who had victories and prizes at the largest international competitions (European, World, Olympic Games), and the second group β MNT group (members of the national team) which included athletes that did not have similar achievements (n = 59). The following biochemical parameters were studied: urea, creatine kinase, ALT, AST, testosterone, cortisol, anabolic index (AI).Results: the absolute values of all metabolites in the examined athletes were within the reference intervals. Statistically significant differences in most of the biochemical parameters were revealed between the compared groups in terms of the level of sports achievements. The SHA group showed a statistically significant shift in relation to MNT group, in direction of increasing the level of metabolites that characterize the predominance of anabolic processes β ALT, testosterone, AI. Metabolite levels, increase which reflects catabolic processes activity and inadequate or insufficient adaptation processes, in the SHA group were significantly lower than in the MNT group. The above changes of the absolute values of biochemical parameters were confirmed by correlation analysis.Conclusions: the obtained results allow us to state the optimal adaptation of this sport, the adequacy of metabolic processes in the group of highly qualified athletes
DNA import competence and mitochondrial genetics
Aim. To understand the mechanism(s) underlying mitochondrial competence for DNA uptake and to exploit these pathways for the development of in vivo models of gene therapy. Methods. DNA uptake into isolated mitochondria from plant or from mutant Saccharomyces cerevisiae defective for mitochondrial proteins and carriers, biochemical approaches and transfection of mammalian cells with DNA bound to mitochondriotropic liposomes. Results. Special focus on the inner membrane showed the involvement of isoforms of the adenine nucleotide translocator and the contribution of proteins controlling mitochondrial morphology in DNA uptake into yeast organelles. Transfection assays led to significant incorporation of a mitochondrial construct into mammalian cells and expression of a marker gene. Conclusions. The data imply that there are multiple mitochondrial DNA import pathways. On the other hand, preliminary results suggest that mitochondriotropic liposomes can deliver DNA to mitochondria in live mammalian cells.ΠΠ΅ΡΠ°. ΠΠΈΠ·Π½Π°ΡΠΈΡΠΈ ΠΌΠ΅Ρ
Π°Π½ΡΠ·ΠΌΠΈ ΠΏΠΎΠ³Π»ΠΈΠ½Π°Π½Π½Ρ ΠΠΠ ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΡΠΌΠΈ Ρ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°ΡΠΈ ΡΡ
Π΄Π»Ρ ΡΠ΄ΠΎΡΠΊΠΎΠ½Π°Π»Π΅Π½Π½Ρ ΡΡΠ½ΡΡΡΠΈΡ
ΠΌΠΎΠ΄Π΅Π»Π΅ΠΉ Π³Π΅Π½Π½ΠΎΡ ΡΠ΅ΡΠ°ΠΏΡΡ in vivo. ΠΠ΅ΡΠΎΠ΄ΠΈ. ΠΠΎΠ³Π»ΠΈΠ½Π°Π½Π½Ρ ΠΠΠ ΡΠ·ΠΎΠ»ΡΠΎΠ²Π°Π½ΠΈΠΌΠΈ ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΡΠΌΠΈ ΡΠΎΡΠ»ΠΈΠ½ Π°Π±ΠΎ ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΡΠΌΠΈ ΠΌΡΡΠ°Π½ΡΠ½ΠΈΡ
Π»ΡΠ½ΡΠΉ Saccharomyces cerevisiae, Π΄Π΅ΡΠ΅ΠΊΡΠ½ΠΈΡ
Π·Π° ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΠ°Π»ΡΠ½ΠΈΠΌΠΈ Π±ΡΠ»ΠΊΠ°ΠΌΠΈ ΡΠ° ΠΏΠ΅ΡΠ΅Π½ΠΎΡΠ½ΠΈΠΊΠ°ΠΌΠΈ, Π±ΡΠΎΡ
ΡΠΌΡΡΠ½Ρ ΠΏΡΠ΄Ρ
ΠΎΠ΄ΠΈ Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΡΡ Π² ΠΊΠ»ΡΡΠΈΠ½ΠΈ ΡΡΠ°Π²ΡΡΠ² ΠΠΠ, Π·Π²βΡΠ·Π°Π½ΠΎΡ Π· ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΠΎΡΡΠΎΠΏΠ½ΠΈΠΌΠΈ Π»ΡΠΏΠΎΡΠΎΠΌΠ°ΠΌΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΠΈ. ΠΡΠ½ΠΎΠ²Π½ΠΈΠΌ ΠΏΡΠ΄ΡΡΠΌΠΊΠΎΠΌ Π²ΠΈΠ²ΡΠ΅Π½Π½Ρ Π²Π½ΡΡΡΡΡΠ½ΡΠΎΡ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½ΠΈ Π²ΠΈΡΠ²ΠΈΠ»ΠΎΡΡ Π²ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½Ρ ΡΠΎΠ³ΠΎ ΡΠ°ΠΊΡΠ°, ΡΠΎ Π΄ΠΎ ΠΏΡΠΎΡΠ΅ΡΡ ΠΏΠ΅ΡΠ΅Π½Π΅ΡΠ΅Π½Π½Ρ ΠΠΠ Π΄ΡΡΠΆΠ΄ΠΆΠΎΠ²ΠΈΠΌΠΈ ΠΎΡΠ³Π°Π½Π΅Π»Π°ΠΌΠΈ Π·Π°Π»ΡΡΠ΅Π½Ρ ΠΊΡΠ»ΡΠΊΠ° ΡΠ·ΠΎΡΠΎΡΠΌ Π°Π΄Π΅Π½ΡΠ½Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΡΡΠ°Π½ΡΠ»ΠΎΠΊΠ°Π·ΠΈ, Π° ΡΠ°ΠΊΠΎΠΆ Π±ΡΠ»ΠΊΠΈ, ΡΠΊΡ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΡΡΡ ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΠ°Π»ΡΠ½Ρ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΡΡ. Π Π΅ΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Ρ
Π· ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΡΡ ΠΠΠ Ρ ΠΊΠ»ΡΡΠΈΠ½ΠΈ ΡΡΠ°Π²ΡΡΠ² Π²ΠΈΡΠ²Π»Π΅Π½ΠΎ Π²Π±ΡΠ΄ΠΎΠ²ΡΠ²Π°Π½Π½Ρ Π² Π½ΠΈΡ
ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΠ°Π»ΡΠ½ΠΎΡ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΡΡ Ρ Π΅ΠΊΡΠΏΡΠ΅ΡΡΡ ΠΌΠ°ΡΠΊΠ΅ΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π°. ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. ΠΡΡΠΈΠΌΠ°Π½Ρ Π΄Π°Π½Ρ Π΄ΠΎΠ·Π²ΠΎΠ»ΡΡΡΡ ΠΏΡΠΈΠΏΡΡΡΠΈΡΠΈ ΡΡΠ½ΡΠ²Π°Π½Π½Ρ Π΄Π΅ΠΊΡΠ»ΡΠΊΠΎΡ
ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡΠ² ΡΠΌΠΏΠΎΡΡΡ ΠΠΠ Ρ ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΡ. ΠΡΠΎΡΠ΅ Ρ ΠΏΠΎΠΏΠ΅ΡΠ΅Π΄Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΈ, ΡΠΊΡ ΠΏΠΎΠΊΠ°Π·ΡΡΡΡ, ΡΠΎ ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΠΎΡΡΠΎΠΏΠ½Ρ Π»ΡΠΏΠΎΡΠΎΠΌΠΈ ΠΌΠΎΠΆΡΡΡ Π±ΡΡΠΈ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Ρ Π΄Π»Ρ Π΄ΠΎΡΡΠ°Π²ΠΊΠΈ ΠΠΠ Ρ ΠΌΡΡΠΎΡ
ΠΎΠ½Π΄ΡΡΡ ΠΊΠ»ΡΡΠΈΠ½ ΡΡΠ°Π²ΡΡΠ² in vivo.Π¦Π΅Π»Ρ. ΠΡΡΡΠ½ΠΈΡΡ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡ ΠΏΠΎΠ³Π»ΠΎΡΠ΅Π½ΠΈΡ ΠΠΠ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΠΌΠΈ ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡ ΠΈΡ
Π΄Π»Ρ ΡΡΠΎΠ²Π΅ΡΡΠ΅Π½ΡΡΠ²ΠΎΠ²Π°Π½ΠΈΡ ΡΡΡΠ΅ΡΡΠ²ΡΡΡΠΈΡ
ΠΌΠΎΠ΄Π΅Π»Π΅ΠΉ Π³Π΅Π½Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ in vivo. ΠΠ΅ΡΠΎΠ΄Ρ. ΠΠΎΠ³Π»ΠΎΡΠ΅Π½ΠΈΠ΅ ΠΠΠ ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΠΌΠΈ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ ΠΈΠ»ΠΈ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΠΌΠΈ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ
Π»ΠΈΠ½ΠΈΠΉ Saccharomyces cerevisiae, Π΄Π΅ΡΠ΅ΠΊΡΠ½ΡΡ
ΠΏΠΎ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΠΌ Π±Π΅Π»ΠΊΠ°ΠΌ ΠΈ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΡΠΈΠΊΠ°ΠΌ, Π±ΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ ΠΈ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ Π² ΠΊΠ»Π΅ΡΠΊΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ
ΠΠΠ, ΡΠ²ΡΠ·Π°Π½Π½ΠΎΠΉ Ρ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΎΡΡΠΎΠΏΠ½ΡΠΌΠΈ Π»ΠΈΠΏΠΎΡΠΎΠΌΠ°ΠΌΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠ½ΠΎΠ²Π½ΡΠΌ ΠΈΡΠΎΠ³ΠΎΠΌ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ Π²Π½ΡΡΡΠ΅Π½Π½Π΅ΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Ρ ΠΎΠΊΠ°Π·Π°Π»ΠΎΡΡ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠΎΠ³ΠΎ ΡΠ°ΠΊΡΠ°, ΡΡΠΎ Π² ΠΏΡΠΎΡΠ΅ΡΡ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΠ° ΠΠΠ Π΄ΡΠΎΠΆΠΆΠ΅Π²ΡΠΌΠΈ ΠΎΡΠ³Π°Π½Π΅Π»Π»Π°ΠΌΠΈ Π²ΠΎΠ²Π»Π΅ΡΠ΅Π½Ρ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΎ ΠΈΠ·ΠΎΡΠΎΡΠΌ Π°Π΄Π΅Π½ΠΈΠ½Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΡΡΠ°Π½ΡΠ»ΠΎΠΊΠ°Π·Ρ, Π° ΡΠ°ΠΊΠΆΠ΅ Π±Π΅Π»ΠΊΠΈ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΠΈΠ΅ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡ. Π ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Ρ
ΠΏΠΎ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΠΠ Π² ΠΊΠ»Π΅ΡΠΊΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ
Π²ΡΡΠ²Π»Π΅Π½Ρ Π²ΡΡΡΠ°ΠΈΠ²Π°Π½ΠΈΠ΅ Π² Π½ΠΈΡ
ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ ΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΌΠ°ΡΠΊΠ΅ΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π°. ΠΡΠ²ΠΎΠ΄Ρ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠΈΡΡ ΡΡΡΠ΅ΡΡΠ²ΠΎΠ²Π°Π½ΠΈΠ΅ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΈΡ
ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΈΠΌΠΏΠΎΡΡΠ° ΠΠΠ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ. ΠΠ΄Π½Π°ΠΊΠΎ Π΅ΡΡΡ ΠΏΡΠ΅Π΄Π²Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ, ΠΏΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡΠΈΠ΅, ΡΡΠΎ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΎΡΡΠΎΠΏΠ½ΡΠ΅ Π»ΠΈΠΏΠΎΡΠΎΠΌΡ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π΄Π»Ρ Π΄ΠΎΡΡΠ°Π²ΠΊΠΈ ΠΠΠ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ
in vivo
DNA import competence and mitochondrial genetics
Aim. To understand the mechanism(s) underlying mitochondrial competence for DNA uptake and to exploit these pathways for the development of in vivo models of gene therapy. Methods. DNA uptake into isolated mitochondria from plant or from mutant Saccharomyces cerevisiae defective for mitochondrial proteins and carriers, biochemical approaches and transfection of mammalian cells with DNA bound to mitochondriotropic liposomes. Results. Special focus on the inner membrane showed the involvement of isoforms of the adenine nucleotide translocator and the contribution of proteins controlling mitochondrial morphology in DNA uptake into yeast organelles. Transfection assays led to significant incorporation of a mitochondrial construct into mammalian cells and expression of a marker gene. Conclusions. The data imply that there are multiple mitochondrial DNA import pathways. On the other hand, preliminary results suggest that mitochondriotropic liposomes can deliver DNA to mitochondria in live mammalian cells
Drug Safety for Children β International Monitoring Data for 50 Years
The review article presents a summary of adverse drug reactions (ADR)Β in children, information about which was received in 1968β2018 in the International database VigiBase (Uppsala monitoring center, UMC).Β Of theΒ 18.4 million Individual Safety Case Reports (ICSR)Β received overΒ 50 years by VigiBase, 1.47 million ICSR contain information on theΒ safety of pharmacotherapy in patients under theΒ age of 18,Β including: 34 510Β reports contain information on ADRΒ in children under theΒ ageΒ of 27 days, 415Β 678Β β in children agedΒ 28 daysΒ to 23 months, 613 676 β aged 2 to 11 years and 405 202 ICSR β in patients aged 12 to 17 years inclusive. During 2018Β 141 655 ICSR ADR of children in VigiBase was received. The most common reason for submitting reports on adverse effects in children was vaccines, antibiotics, non-steroidal antiinflammatory drugs, analgesics-antipyretics, anti-acne and valproic acid. The most common side effects of drugs in children were the following ADR: hyperthermia, rash, vomiting, nausea, urticaria, diarrhea, itching, headache, erythema at injection site, convulsion. Separate data on 6 age groups about 10 most frequent ADR in children andΒ about 10 medicines which ICSR most often arrived in VigiBase for 50 years and for 2018Β are given