8 research outputs found
Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism.
Lima1 is an extensively studied prognostic marker of malignancy and is also considered to be a tumour suppressor, but its role in a developmental context of non-transformed cells is poorly understood. Here, we characterise the expression pattern and examined the function of Lima1 in mouse embryos and pluripotent stem cell lines. We identify that Lima1 expression is controlled by the naĂŻve pluripotency circuit and is required for the suppression of membrane blebbing, as well as for proper mitochondrial energetics in embryonic stem cells. Moreover, forcing Lima1 expression enables primed mouse and human pluripotent stem cells to be incorporated into murine pre-implantation embryos. Thus, Lima1 is a key effector molecule that mediates the pluripotency control of membrane dynamics and cellular metabolism
Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause
The epiblast, which provides the foundation of the future body, is actively reshaped during early embryogenesis, but the reshaping mechanisms are poorly understood. Here, using a 3D in vitro model of early epiblast development, we identify the canonical Wnt/ÎČ-catenin pathway and its central downstream factor Esrrb as the key signalling cascade regulating the tissue-scale organization of the murine pluripotent lineage. Although in vivo the Wnt/ÎČ-catenin/Esrrb circuit is dispensable for embryonic development before implantation, autocrine Wnt activity controls the morphogenesis and long-term maintenance of the epiblast when development is put on hold during diapause. During this phase, the progressive changes in the epiblast architecture and Wnt signalling response show that diapause is not a stasis but instead is a dynamic process with underlying mechanisms that can appear redundant during transient embryogenesis
Correlative Light and Electron Microscopy of Rare Cell Populations in Zebrafish Embryos Using Laser Marks
Surface Chemistry of Quantum Dots Determines Their Behavior in Postischemic Tissue
The behavior of quantum dots (QDs) in the microvasculature and their impact on inflammatory reactions under pathophysiological conditions are still largely unknown. Therefore, we designed this study to investigate the fate and effects of surface-modified QDs in postischemic skeletal and heart muscle. Under these pathophysiological conditions, amine-modified QDs, but not carboxyl-QDs, were strongly associated with the vessel wall of postcapillary venules and amplified ischemia-reperfusion-elicited leukocyte transmigration. Importantly, strong association of amine-QDs with microvessel walls was also present in the postischemic myocardium. As shown by electron microscopy and verified by FACS analyses, amine-modified QDs, but not carboxyl-QDs, were associated with endogenous microparticles. At microvessel walls, these aggregates were attached to endothelial cells. Taken together, we found that both the surface chemistry of QDs and the underlying tissue conditions (<i>i.e.</i>, ischemia-reperfusion) strongly determine their uptake by endothelial cells in microvessels, their association to endogenous microparticles, as well as their potential to modify inflammatory processes. Thus, this study strongly corroborates the view that the surface chemistry of nanomaterials and the physiological state of the tissue are crucial for the behavior of nanomaterials <i>in vivo.</i
Surface Chemistry of Quantum Dots Determines Their Behavior in Postischemic Tissue
The behavior of quantum dots (QDs) in the microvasculature and their impact on inflammatory reactions under pathophysiological conditions are still largely unknown. Therefore, we designed this study to investigate the fate and effects of surface-modified QDs in postischemic skeletal and heart muscle. Under these pathophysiological conditions, amine-modified QDs, but not carboxyl-QDs, were strongly associated with the vessel wall of postcapillary venules and amplified ischemia-reperfusion-elicited leukocyte transmigration. Importantly, strong association of amine-QDs with microvessel walls was also present in the postischemic myocardium. As shown by electron microscopy and verified by FACS analyses, amine-modified QDs, but not carboxyl-QDs, were associated with endogenous microparticles. At microvessel walls, these aggregates were attached to endothelial cells. Taken together, we found that both the surface chemistry of QDs and the underlying tissue conditions (<i>i.e.</i>, ischemia-reperfusion) strongly determine their uptake by endothelial cells in microvessels, their association to endogenous microparticles, as well as their potential to modify inflammatory processes. Thus, this study strongly corroborates the view that the surface chemistry of nanomaterials and the physiological state of the tissue are crucial for the behavior of nanomaterials <i>in vivo.</i
The surface chemistry determines the spatioâtemporal interaction dynamics of quantum dots in atherosclerotic lesions
Innate immunity mediated by TLR9 modulates pathogenicity in an animal model of multiple sclerosis
Inflammatory diseases of the CNS, such as MS and its animal model EAE, are characterized by infiltration of activated lymphocytes and phagocytes into the CNS. Within the CNS, activation of resident cells initiates an inflammatory cascade, leading to tissue destruction, demyelination, and neurologic deficit. TLRs recognize microbes and are pivotal mediators of innate immunity. Within the CNS, augmented TLR expression during EAE is observed, even in the absence of any apparent microbial involvement. To determine the functional relevance of this phenomenon during sterile autoimmunity, we studied the role of different TLRs as well as their common signaling adaptor MyD88 in the development of EAE. We found that MyD88(â/â) mice were completely EAE resistant. Surprisingly, this protection is partly due to engagement of the CpG receptor TLR9. Restricting the MyD88 or TLR9 mutation to host radio-resistant cells, including the cells within the CNS, revealed that engagement of radio-resistant cells modulated the disease course and histopathological changes. Our data clearly demonstrate that both TLR9 and MyD88 are essential modulators of the autoimmune process during the effector phase of disease and suggest that endogenous âdanger signalsâ modulate the disease pathogenesis
The Endothelial Glycocalyx Controls Interactions of Quantum Dots with the Endothelium and Their Translocation across the BloodâTissue Border
Advances
in the engineering of nanoparticles (NPs), which represent
particles of less than 100 nm in one external dimension, led to an
increasing utilization of nanomaterials for biomedical purposes. A
prerequisite for their use in diagnostic and therapeutic applications,
however, is the targeted delivery to the site of injury. Interactions
between blood-borne NPs and the vascular endothelium represent a critical
step for nanoparticle delivery into diseased tissue. Here, we show
that the endothelial glycocalyx, which constitutes a glycoproteinâpolysaccharide
meshwork coating the luminal surface of vessels, effectively controls
interactions of carboxyl-functionalized quantum dots with the microvascular
endothelium. Glycosaminoglycans of the endothelial glycocalyx were
found to physically cover endothelial adhesion and signaling molecules,
thereby preventing endothelial attachment, uptake, and translocation
of these nanoparticles through different layers of the vessel wall.
Conversely, degradation of the endothelial glycocalyx promoted interactions
of these nanoparticles with microvascular endothelial cells under
the pathologic condition of ischemiaâreperfusion, thus identifying
the injured endothelial glycocalyx as an essential element of the
bloodâtissue border facilitating the targeted delivery of nanomaterials
to diseased tissue