Description of the bias introduced by the transition from Conventional Manual Measurements to Automatic Weather Station through the analysis of European and American parallel datasets (+ Australia, Israel & Kyrgyzstan)

Presentación realizada en: 10th EUMETNET Data Management Workshop celebrado en St. Gallen, Suiza, del 28 al 30 de octubre de 2015.In this work, we approach the description of the biases introduced by automation in temperature records. This is one of the first studies in the framework of The Parallel Observations Scientific Team (POST). POST is a newly created group of the International Surface Temperature Initiative (ISTI), with the support of the World Meteorological Organization (WMO). The goals of POST (http://www.surfacetemperatures.org/databank/parallel_measurements) are the study of climate data inhomogeneities at the daily and sub-daily level through the compilation and analysis of parallel measurements. Long instrumental climate records are usually affected by non-climatic changes, due to, e.g., relocations and changes in instrumentation, instrument height or data collection and manipulation procedures. These so-called inhomogeneities distort the climate signal and can hamper the assessment of trends and variability. Thus to study climatic changes we need to accurately distinguish non-climatic and climatic signals. The most direct way to study the influence of non-climatic changes on the distribution and to understand the reasons for these biases is the analysis of parallel measurements. A parallel measurement is composed of two or more time series, which measure a climatic variable with two different systems (for example, Montsouris and Stevenson Screens) or in two different locations (for example, city centre and airport). They mimic the situation “before” and “after” a homogeneity break. Most parallel measurements are obtained from collocated or nearly collocated series and can help us to understand the size and shape of different typical sources of inhomogeneity, which affect the climate series. Here we study the transition from conventional temperature manual measurements (CON) to Automatic Weather Stations (AWS), using several parallel datasets distributed over Europe and America. The variables studied in the analysis presented here are daily maximum and minimum temperature. First of all, the metadata – when available - is gathered to gain knowledge on the exact setting of the parallel series. Secondly, the difference (temperature) series AWS-CON are submitted to quality control, to remove obvious errors and inspected to detect internal inhomogeneities and split if necessary. In a third step, each segment is studied to understand the bias introduced by the transition, its seasonality as well as changes in the empirical distributions. When additional variables are available, an attempt is made to study the effects of other variables on the observed biases.With the support of Grant CGL2012-32193, Ministerio de Economía y Competitividad, MINECO, España and FP7-SPACE-2013-1 grand 607193, Uncertainties in Ensembles of Regional Reanalyses (UERRA)

Acute Stress Regulates Sex-Related Molecular Responses in the Human Jejunal Mucosa: Implications for Irritable Bowel Syndrome

Estrés agudo; Barrera intestinal; SexoEstrès agut; Barrera intestinal; SexeCute stress; Intestinal barrier; SexIrritable bowel syndrome (IBS) is a prevalent gastrointestinal disorder linked to intestinal barrier dysfunction and life stress. We have previously reported that female sex per se determines an increased susceptibility to intestinal barrier dysfunction after cold pain stress (CPS). We aimed to identify sex-related molecular differences in response to CPS in healthy subjects to understand the origin of sex bias predominance in IBS. In 13 healthy males and 21 females, two consecutive jejunal biopsies were obtained using Watson’s capsule, at baseline, and ninety minutes after CPS. Total mucosal RNA and protein were isolated from jejunal biopsies. Expression of genes related to epithelial barrier (CLDN1, CLDN2, OCLN, ZO-1, and ZO-3), mast cell (MC) activation (TPSAB1, SERPINA1), and the glucocorticoid receptor (NR3C1) were analyzed using RT-qPCR. NR3C1, ZO-1 and OCLN protein expression were evaluated through immunohistochemistry and western blot, and mucosal inflammation through MC, lymphocyte, and eosinophil numbering. Autonomic, hormonal, and psychological responses to CPS were monitored. We found an increase in jejunal MCs, a reduced CLDN1 and OCLN expression, and an increased CLDN2 and SERPINA1 expression 90 min after CPS. We also found a significant decrease in ZO-1, OCLN, and NR3C1 gene expression, and a decrease in OCLN protein expression only in females, when compared to males. CPS induced a significant increase in blood pressure, plasma cortisol and ACTH, and subjective stress perception in all participants. Specific and independent sex-related molecular responses in epithelial barrier regulation are unraveled by acute stress in the jejunum of healthy subjects and may partially explain female predominance in IBS.Supported in part by Fondo Europeo de Desarrollo Regional (FEDER), Fondo de Investigación Sanitaria and Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, Subdirección General de Investigación Sanitaria, Ministerio de Economiá y Competitividad: CM08/00229 (BL); CM10/00155 (MP); EII2011-0035, CD15/00010, and MV17-00043 (BKRJ.); FI12/00254 (ESR.), PI17/0190 (JS), PI12/00314 and PI15/00301 (CAC), CIBEREHD CB06/04/0021 (JS, CAC.); Vall d’Hebron Institut de Recerca, Programa de becas predoctorales Amics de Vall d’Hebron: PRED-VHIR-2014-018 (MF), PRED-VHIR-2016-53 34 (CPC.)

Creación literaria

Tras la elaboración de una revista literaria en las Jornadas Culturales del curso pasado, el claustro comprueba la necesidad de sistematizar el trabajo de creación literaria. Los objetivos son favorecer y desarrollar la creatividad para lograr el máximo desarrollo individual; explorar las posibilidades expresivas orales y escritas de la lengua para desarrollar la sensibilidad estética; y utilizar la lectura como fuente de placer, de información y de aprendizaje, y como medio de perfeccionamiento y enriquecimiento lingüístico y personal. Se publica una revista literaria por trimestre dedicada al cuento, la poesía y el relato corto, con una selección de trabajos en cada aula. Los alumnos escriben, dibujan y escenifican cuentos y poesías, acuden a funciones de teatro y participan en la Muestra de Teatro Escolar. Cada aula realiza el Libro Viajero con la colaboración de las familias que añaden sus producciones literarias y artísticas. Además se realizan actividades semanales de animación a la lectura y pruebas de escritura para los niños. Y los profesores acuden a cursos de formación sobre lectura eficaz y creacción artística. Durante las jornadas culturales se publica el diario Madrid en punto. La evaluación es trimestral y se valora la calidad y adecuación de los trabajos elaborados, la participación y la repercusión en la actividad del centro.Madrid (Comunidad Autónoma). Consejería de EducaciónMadridMadrid (Comunidad Autónoma). Subdirección General de Formación del Profesorado. CRIF Las Acacias; General Ricardos 179 - 28025 Madrid; Tel. + 34915250893ES

Acute Stress Regulates Sex-Related Molecular Responses in the Human Jejunal Mucosa: Implications for Irritable Bowel Syndrome

Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disorder linked to intestinal barrier dysfunction and life stress. We have previously reported that female sex per se determines an increased susceptibility to intestinal barrier dysfunction after cold pain stress (CPS). We aimed to identify sex-related molecular differences in response to CPS in healthy subjects to understand the origin of sex bias predominance in IBS. In 13 healthy males and 21 females, two consecutive jejunal biopsies were obtained using Watson’s capsule, at baseline, and ninety minutes after CPS. Total mucosal RNA and protein were isolated from jejunal biopsies. Expression of genes related to epithelial barrier (CLDN1, CLDN2, OCLN, ZO-1, and ZO-3), mast cell (MC) activation (TPSAB1, SERPINA1), and the glucocorticoid receptor (NR3C1) were analyzed using RT-qPCR. NR3C1, ZO-1 and OCLN protein expression were evaluated through immunohistochemistry and western blot, and mucosal inflammation through MC, lymphocyte, and eosinophil numbering. Autonomic, hormonal, and psychological responses to CPS were monitored. We found an increase in jejunal MCs, a reduced CLDN1 and OCLN expression, and an increased CLDN2 and SERPINA1 expression 90 min after CPS. We also found a significant decrease in ZO-1, OCLN, and NR3C1 gene expression, and a decrease in OCLN protein expression only in females, when compared to males. CPS induced a significant increase in blood pressure, plasma cortisol and ACTH, and subjective stress perception in all participants. Specific and independent sex-related molecular responses in epithelial barrier regulation are unraveled by acute stress in the jejunum of healthy subjects and may partially explain female predominance in IBS