14 research outputs found
Bacterial strains.
<p>Indicated strains and plasmids are from Stratagene, La Jolla, CA, USA.</p
The amino acids of proposed proton transfer pathway are highly conserved.
<p>T.r.: <i>Thiocapsa roseopersicina</i> HynL, A.v.: <i>Allochromatium vinosum</i> HydL, R.c.: <i>Rhodobacter capsulatus</i> HupL, M.c.: <i>Methylococcus capsulatus</i>, B.j.: <i>Bradyrhizobium japonicum</i> HupL, Rh.l.: <i>Rhizobium leguminosarum</i> HupL, R.e.: <i>Ralstonia eutropha</i> H16 HoxG, D.v.: <i>Desulfovibrio vulgaris</i> Miyazaki F HynB, D.g.: <i>Desulfovibrio gigas</i> HynB, D.f.: <i>Desulfovibrio fructosovorans</i> HynB. The upper and lower numbering refers to the large subunits of <i>T. roseopersicina</i> and <i>D. vulgaris</i> enzymes, respectively.</p
Mutagenesis primers used for creating new mutants.
<p>Nucleotide triplets coding mutated amino acids are bold.</p
Arrangement of conserved histidines.
<p>T.r.: <i>Thiocapsa roseopersicina</i> HynL, A.v.: <i>Allochromatium vinosum</i> HydL, R.c.: <i>Rhodobacter capsulatus</i> HupL, M.c.: <i>Methylococcus capsulatus</i>, B.j.: <i>Bradyrhizobium japonicum</i> HupL, Rh.l.: <i>Rhizobium leguminosarum</i> HupL, R.e.: <i>Ralstonia eutropha</i> H16 HoxG, D.v.: <i>Desulfovibrio vulgaris</i> Miyazaki F HynB, D.g.: <i>Desulfovibrio gigas</i> HynB, D.f.: <i>Desulfovibrio fructosovorans</i> HynB. The numbering refers to the <i>T. roseopersicina</i> HynL subunit.</p
<i>In vivo</i> and <i>in vitro</i> hydrogenase activity of <i>Thiocapsa roseopersicina</i> HynSL enzyme.
<p><i>In vivo</i> and <i>in vitro</i> hydrogenase activity of <i>Thiocapsa roseopersicina</i> HynSL enzyme.</p
Change of specific conductivity as function of surfactant concentration.
<p>Change of specific conductivity as function of surfactant concentration.</p
The average SDS content (x2) of the micelle (upper panel) and their relative shape anisotropy (lower panel, Κ2) as a function of the cluster size.
<p>The average SDS content (x2) of the micelle (upper panel) and their relative shape anisotropy (lower panel, Κ2) as a function of the cluster size.</p
Temperature dependent critical micelle concentration obtained by fluorimetric and tensiometric experiments (noted by cmc<sup>ex</sup><sub>f</sub>(T) and cmc<sup>ex</sup><sub>t</sub>(T), respectively), ideal critical micelle concentration (cmc<sup>id</sup>), mole fraction of NaCA (<i>x<sub>1</sub></i>) in the mixed micelle and interaction parameter between the building units (β<sub>1,2</sub>) as a function of temperature for the binary mixture of NaCA and SDS (1∶1).
<p><b>cmc<sub>1,f</sub> (T)</b>- cmc of pure NaCA measured by fluorimetry.</p><p><b>cmc<sub>2,</sub></b><sub><b><i>f</i></b></sub><i> </i><b><i>(T)</i></b>- cmc of pure SDS measured by fluorimetry.</p
The initial structure of the coarse-grained molecular dynamics simulation: CA, SDS and Na+ are represented in red, yellow and blue van der Waals representation, respectively.
<p>The initial structure of the coarse-grained molecular dynamics simulation: CA, SDS and Na+ are represented in red, yellow and blue van der Waals representation, respectively.</p