Flight Servicing of Robotic Refueling Mission 3

The Robotic Refueling Mission 3 (RRM3) payload launched aboard a SpaceX rocket en route to the International Space Station on December 5th, 2018. The Goddard Space Flight Center designed payload carried approximately 50 liters of liquid methane onboard, with a mission to demonstrate long term storage and transfer of the cryogenic fluid in microgravity. Kennedy Space Center (KSC) was tasked to design, fabricate, test, and operate a system equipped to fill an RRM3 dewar with liquid methane prior to launch. Though KSC has a rich history of fueling rockets and payloads, no such operations had previously been accomplished using liquid methane. As such, all of the hardware and processes had to be developed from scratch. The completed ground system design, along with the verification and validation testing will be outlined in this paper. Several challenges that were met and overcome during procurement of the high purity methane are described. In addition, budget restrictions prohibited fueling operations from occurring in traditional processing facilities. The unique and creative solutions which were required to maintain payload cleanliness during cryogenic servicing are also detailed

Flight Servicing of Robotic Refueling Mission 3

The Robotic Refueling Mission 3 (RRM3) payload launched aboard a SpaceX rocket en route to the International Space Station on December 5th, 2018. The Goddard Space Flight Center designed payload carried approximately 50 liters of liquid methane onboard, with a mission to demonstrate long term storage and transfer of the cryogenic fluid in microgravity. Kennedy Space Center (KSC) was tasked to design, fabricate, test, and operate a system equipped to fill an RRM3 dewar with liquid methane prior to launch. Though KSC has a rich history of fueling rockets and payloads, no such operations had previously been accomplished using liquid methane. As such, all of the hardware and processes had to be developed from scratch. The completed ground system design, along with the verification and validation testing will be outlined in this paper. Several challenges that were met and overcome during procurement of the high purity methane are described. In addition, budget restrictions prohibited fueling operations from occurring in traditional processing facilities. The unique and creative solutions which were required to maintain payload cleanliness during cryogenic servicing are also detailed

Flight Servicing of Robotic Refueling Mission 3

The Robotic Refueling Mission 3 (RRM3) payload launched aboard a SpaceX rocket en route to the International Space Station on December 5th, 2018. The Goddard Space Flight Center designed payload carried approximately 50 liters of liquid methane onboard, with a mission to demonstrate long term storage and transfer of the cryogenic fluid in microgravity. Kennedy Space Center (KSC) was tasked to design, fabricate, test, and operate a system equipped to fill an RRM3 dewar with liquid methane prior to launch. Though KSC has a rich history of fueling rockets and payloads, no such operations had previously been accomplished using liquid methane. As such, all of the hardware and processes had to be developed from scratch. The completed ground system design, along with the verification and validation testing will be outlined in this paper. Several challenges that were met and overcome during procurement of the high purity methane are described. In addition, budget restrictions prohibited fueling operations from occurring in traditional processing facilities. The unique and creative solutions which were required to maintain payload cleanliness during cryogenic servicing are also detailed

Phase 1 doseâ finding study of metformin in combination with concurrent cisplatin and radiotherapy in patients with locally advanced head and neck squamous cell cancer

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/153201/1/cncr32539.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/153201/2/cncr32539_am.pd

Corresponding States of Structural Glass Formers

The variation with respect to temperature T of transport properties of 58 fragile structural glass forming liquids (68 data sets in total) are analyzed and shown to exhibit a remarkable degree of universality. In particular, super-Arrhenius behaviors of all super-cooled liquids appear to collapse to one parabola for which there is no singular behavior at any finite temperature. This behavior is bounded by an onset temperature To above which liquid transport has a much weaker temperature dependence. A similar collapse is also demonstrated, over the smaller available range, for existing numerical simulation data.Comment: 6 pages, 2 figures. Updated References, Table Values, Submitted for Publicatio

HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis

Cytokinesis, the final phase of cell division, is necessary to form two distinct daughter cells with correct distribution of genomic and cytoplasmic materials. Its failure provokes genetically unstable states, such as tetraploidization and polyploidization, which can contribute to tumorigenesis. Aurora-B kinase controls multiple cytokinetic events, from chromosome condensation to abscission when the midbody is severed. We have previously shown that HIPK2, a kinase involved in DNA damage response and development, localizes at the midbody and contributes to abscission by phosphorylating extrachromosomal histone H2B at Ser14. Of relevance, HIPK2-defective cells do not phosphorylate H2B and do not successfully complete cytokinesis leading to accumulation of binucleated cells, chromosomal instability, and increased tumorigenicity. However, how HIPK2 and H2B are recruited to the midbody during cytokinesis is still unknown. Here, we show that regardless of their direct (H2B) and indirect (HIPK2) binding of chromosomal DNA, both H2B and HIPK2 localize at the midbody independently of nucleic acids. Instead, by using mitotic kinase-specific inhibitors in a spatio-temporal regulated manner, we found that Aurora-B kinase activity is required to recruit both HIPK2 and H2B to the midbody. Molecular characterization showed that Aurora-B directly binds and phosphorylates H2B at Ser32 while indirectly recruits HIPK2 through the central spindle components MgcRacGAP and PRC1. Thus, among different cytokinetic functions, Aurora-B separately recruits HIPK2 and H2B to the midbody and these activities contribute to faithful cytokinesis

Mutational profiles of persistent/recurrent laryngeal squamous cell carcinoma

BackgroundWe sought to describe targeted DNA sequencing data of persistent/recurrent laryngeal squamous cell carcinoma (LSCC) and to compare gene‐specific alteration frequencies with that of primary, untreated LSCC specimens from The Cancer Genome Atlas (TCGA).MethodsThe tumors of 21 patients with persistent/recurrent LSCC were subjected to targeted DNA sequencing using the Ion AmpliSeq Comprehensive Cancer Panel. Gene‐specific alteration frequencies were compared (Chi‐Square test) to primary, untreated LSCC sequencing data from TCGA using the cBioPortal platform.ResultsPersistent/recurrent LSCC was characterized by a high rate of inactivating alterations in TP53 (38.1%) and CDKN2A (33%), amplification events of CCND1 (19.1%), and ERBB2 (14.3%), and NOTCH1 (19.1%) mutations. Comparison of primary vs persistent/recurrent LSCC revealed significant differences in alteration frequencies of eight critical genes: BAP1, CDKN2A, DCUN1D1, MSH2, MTOR, PIK3CA, TET2, and TP53.ConclusionsOur results provide preliminary support for a distinct mutational profile of persistent/recurrent LSCC that requires validation in larger cohorts.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147873/1/hed25444.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147873/2/hed25444_am.pd

Impact of American Joint Committee on Cancer Eighth Edition clinical stage and smoking history on oncologic outcomes in human papillomavirus‐associated oropharyngeal squamous cell carcinoma

BackgroundThe purpose of this study was to evaluate the AJCC eighth edition clinical staging system for human papillomavirus (HPV)‐associated oropharyngeal squamous cell carcinoma and to further understand how clinical stage and smoking history affect oncologic outcomes. The purpose of this study was to present the understanding of how clinical stage and smoking history affect oncologic outcomes in human papillomavirus (HPV)‐associated oropharyngeal squamous cell carcinoma (SCC) is critical for selecting patients for treatment deintensification.MethodsKaplan‐Meier and Cox regression were used to evaluate overall survival (OS), locoregional recurrence‐free survival (LRFS), and distant recurrence‐free survival (DRFS). Concordance statistics (C‐indices) were used to compare discriminating ability.ResultsThe OS and DRFS but not LRFS were significantly distributed using the American Joint Committee on Cancer (AJCC) seventh and eighth editions criteria. The C‐indices for OS, LRFS, and DRFS were 0.57, 0.54, and 0.60, respectively, using the AJCC seventh edition, and 0.63, 0.53, and 0.65, respectively, using the AJCC eighth edition. On multivariate analysis, 1 + pack‐year smoking history correlated with OS (hazard ratio [HR] 1.96; 95% confidence interval [CI] 1.2‐3.1; P < .01) but not LRFS or DRFS.ConclusionThese results support implementation of the AJCC eighth edition for HPV‐associated oropharyngeal SCC. Clinical stage may be more important than smoking history in selection for deintensification.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/148352/1/hed25336_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148352/2/hed25336.pd