2 research outputs found
Maize Transposable Elements Ac/Ds as Insertion Mutagenesis Tools in Candidaalbicans
In non-model systems genetic research is often limited by the lack of
techniques for the generation and identification of gene mutations. One
approach to overcome this bottleneck is the application of transposons for
gene tagging. We have established a two-element transposon tagging system,
based on the transposable elements Activator (Ac)/Dissociation (Ds) from
maize, for in vivo insertion mutagenesis in the fungal human pathogen Candida
albicans. A non-autonomous Ds transposon carrying a selectable marker was
constructed into the ADE2 promoter on chromosome 3 and a codon usage-adapted
Ac transposase gene was inserted into the neutral NEUT5L locus on chromosome
5. In C. albicans cells expressing the transposase the Ds element efficiently
excised and reintegrated elsewhere in the genome, which makes the Ac/Ds
transposons promising tools for saturating insertion mutagenesis in clinical
strains of C. albicans
Gene Essentiality Analyzed by In Vivo Transposon Mutagenesis and Machine Learning in a Stable Haploid Isolate of Candida albicans
This work was supported by European Research Council Advanced Award 340087 (RAPLODAPT) to J.B., the Dahlem Centre of Plant Sciences (DCPS) of the Freie Universität Berlin (R.K.), Israel Science Foundation grant no. 715/18 (R.S.), the Wellcome Trust (grants 086827, 075470, 101873, and 200208) and the MRC Centre for Medical Mycology (N006364/1) (N.A.R.G.). Data availability.All of the code and required dependencies for analysis of the TnSeq data are available at https://github.com/berman-lab/transposon-pipeline. Library insertion sequences are available at NCBI under project PRJNA490565 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA490565). Datasets S1 through S9 are available at https://doi.org/10.6084/m9.figshare.c.4251182.Peer reviewedPublisher PD