Indikatoren zur Ausbildung im Hochschulbereich : Studie zum Innovationssystem Deutschlands Nr. 10-2003

Dieser Bericht wurde im Rahmen der erweiterten Berichterstattung zur technologischen Leistungsfähigkeit Deutschlands im Auftrag des Bundesministeriums für Bildung und Forschung erstellt

Indikatoren zur Ausbildung im Hochschulbereich: Studie zum Innovationssystem Deutschlands Nr. 10-2003

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The present: an "unknown time" in the German Kaiserreich around 1900

In her contribution, Rothauge focuses on assumptions of ‘the present’ in the German Kaiserreich around 1900. Historical sources reveal that many contemporaries considered present time(s) to be highly dynamic and heterogeneous, thus confusing and partly ‘unknown’. Rothauge links this to several official initiatives preoccupied with synchronising different time regimes. She argues that these attempts initially led to a yet again increased pluralisation of both the notions and uses of ‘the present’. According to this, the master narrative of (high) modernity as being characterised by just one specific temporal experience, namely that of an ever increasing acceleration, needs to be looked at in a more differentiated way, paying more attention to the fact that people actively take part in the construction of temporal discourses

Time-resolved crystallography captures light-driven DNA repair

International audiencePhotolyase is an enzyme that uses light to catalyze DNA repair. To capture the reaction intermediates involved in the enzyme’s catalytic cycle, we conducted a time-resolved crystallography experiment. We found that photolyase traps the excited state of the active cofactor, flavin adenine dinucleotide (FAD), in a highly bent geometry. This excited state performs electron transfer to damaged DNA, inducing repair. We show that the repair reaction, which involves the lysis of two covalent bonds, occurs through a single-bond intermediate. The transformation of the substrate into product crowds the active site and disrupts hydrogen bonds with the enzyme, resulting in stepwise product release, with the 3′ thymine ejected first, followed by the 5′ base