1 research outputs found
Improved Isotopic Profiling by Pure Shift Heteronuclear 2D <i>J</i>‑Resolved NMR Spectroscopy
Quantitative information
on the carbon isotope content of metabolites
is essential for flux analysis. Whereas this information is in principle
present in proton NMR spectra through both direct and long-range heteronuclear
coupling constants, spectral overlap and homonuclear coupling constants
both hinder its extraction. We demonstrate here how pure shift 2D <i>J</i>-resolved NMR spectroscopy can simultaneously remove the
homonuclear couplings and separate the chemical shift information
from the heteronuclear coupling patterns. We demonstrate the power
of this method on cell lysates from different bacterial cultures and
investigate in detail the branched chain amino acid biosynthesis