Significant association between high serum CCL5 levels and better disease‐free survival of patients with early breast cancer

Analysis of anticancer immunity aids in assessing the prognosis of patients with breast cancer. From 250 operated breast cancers, we focused on serum levels of C-C motif chemokine ligand 5 (CCL5), which is involved in cancer immune reactions. Serum levels of CCL5 were measured using a cytometric bead-based immunoassay kit and CCL5 expression in cancer cells was determined using immunohistochemical staining. In addition, mRNA in cancer and stromal cells was analyzed by microdissection and comparison with the public dataset. Disease-free survival (DFS) of patients with high CCL5 levels (cut-off, 13.87 ng/mL; n = 192) was significantly better than those with low CCL5 levels (n = 58; hazard ratio, 0.20; 95% confidence interval, 0.10- 0.39; P < .0001). An improved overall survival was observed in patients with high CCL5 levels compared to those with low CCL5 levels (P = .024). On the contrary, high immunohistochemical expression of CCL5 in cancer cells was significantly associated with decreased DFS. As serum CCL5 levels did not correlate with CCL5 expression in cancer cells and the relative expression of mRNA CCL5 was elevated in stromal cells in relation to cancer cells, serum CCL5 might be derived not from cancer cells, but from stromal cells. Expression of CCL5 in serum, but not in cancer cells, might contribute to improved patient prognosis mediating through not only immune reaction, but through other mechanisms. Determination of circulating CCL5 levels could be useful for predicting patient prognosis

快適感に及ぼす靴素材と水分量の影響

The effect of shoe materials and moisture content on wear comfort while walking and resting has been studied by the experiment using natural and artificial leather shoe. Temperature and absolute humidity between the first and the second toe were the highest. Temperature and absolute humidity between the first and the second toe, and those on the skin surface at the arch of the foot had a significant difference. The significant difference was found in those between the first and th second toe, and those on the stocking at the arch of the foot. In the case of wearing leather and artificial leather shoe, temperature, absolute humidity, thermal sensation, sense of sweating, sense of soaking and discomfort sensation, these four kinds of subjective sensations were about the same. There was high connection between those subjective sensations and the moisture content between the first and the second toe. Wearing propaties of natural and artificial leather shoe were about the same under medium temperature and absolute humidity

The self-esteem and the activities of daily living in inpatients after an ischemic stroke

急性期の脳梗塞患者の自尊感情と日常生活動作の関連を明らかにするために,入院中の脳梗塞患者150人に調査協力を依頼した.調査協力の同意が得られなかった患者25人と調査前に退院された患者17人を除き,108人(72.0%)(男性74人,女性34人,平均年齢64.5(SD 12.5)才)にアンケート調査を実施した.自尊感情(Rosenberg のSelf-Esteem,以下RSE と記載)は平均29.9(SD 6.1)点,日常生活動作(Barthel index,以下BI と記載)は平均89.4(SD 16.0)点であり,RSE とBI の間にはBI が低いとRSE が低いという正の相関がみられた(Spearman のρ= 0.461,p < 0.001).また,BI が低いことと「女性である」「高齢である」「入院時の機能障害が重い」の関連が示唆された.急性期には,脳梗塞患者の日常生活動作の自立に向けた援助と,自尊感情を高める介入が必要であると考えられた.The purpose of this study was to investigate the relationship between self-esteem and the activities of daily living in inpatients after an ischemic stroke. The level of Rosenberg's Self-esteem and the Barthel index were examined. There were 108 respondents (74 males and 34 females). Based on these results, selfesteem was significantly correlated to the Barthel Index (Spearman's ρ=0.461, p<0.001). Findings indicated that low self-esteem related to dependence, and the level of dependence showed a negative association with women, the elderly and severe disability. These results suggested the necessity of rehabilitation for re-acquiring the skills of daily living and enhancement of self-esteem in patients who are dependent.広島保健学学会 特別講

ニンチショウ コウレイシャ ノ ショクジセイ テイケツアツ ト ジリツ シンケイ カツドウ ト ノ カンレン

【目的】認知症高齢者の食事性低血圧(PPH)と自律神経活動の関連と,PPHの発現頻度,自律神経活動との関連を明らかにする.【方法】歩行可能な入院中の認知症高齢者を対象に,食前と食後の血圧,脈拍,指尖脈波を用いた自律神経活動測定を,座位にて5日間実施した,【結果】対象者は男性11人,女性20人,合計31人で,平均年齢85.42±6.36歳であった.病名別ではアルツハイマー型認知症(AD)23人,レビー小体型認知症(DLB)6人,ウェルニッケ･コルサコフ症候群1人,脳血管性認知症1人であった.対象者の61%にPPHの発現があった.ADの70%にPPHの発現があった.PPH発現時のLn(LF/HF)は食前0.93±0.24,食後0.81±0.17(p＜0.05)で有意差があった.PPHの発現なし時のLn(LF/HF)の平均値は食前0.93±0.28,食後0.92±0.26であり,有意差はなかった.【考察】PPH発現のケースでは食前･後のLn(LF/HF)に差があり,PPH発現なしではLn(LF/HF)に差がないことから,PPHの発現ケースには心臓交感神経活動が食前と比して食後に有意に減弱しており,交感神経活動にPPHが影響していることが示唆された.Purpose : This study aims to clarify the correlation between postprandial hypotension (PPH) and autonomicnervous activity in elderly people with dementia.Method : We examined the autonomic nervous activities of ambulatory patients by measuring their blood pressure, pulse and finger plethysmogram before and after meals for five days.Results : There were 11 male and 20 female subjects with a median age of 85.42 ± 6.36. We had 23 patientswith AD, six with DLB, one with Wernicke-Korsakoff, and one with cerebrovascular dementia. 61 percent of the subjects showed symptoms of PPH. 70 percent of the patients with AD showed symptoms of PPH. The value of Ln (LF / HF)with PPH was 0.93 ± 0.24 before meal and 0.81 ± 0.17 (p<0.05) after meal, while the average value of Ln(LF / HF)without PPH was 0.93 ± 0.28 before meal and 0.92 ± 0.26 after meal. The comparison showed no significant differences.Discussion : No disparity was found in autonomic nervous activities among patients without PPH symptoms.It is suggested that PPH affects sympathetic nerve activity since we observed a significant decrease of sympathetic nerve activities after meals

Beam and SKS spectrometers at the K1.8 beam line

High-resolution spectrometers for both incident beams and scattered particles have been constructed at the K1.8 beam line of the Hadron Experimental Facility at J-PARC. A point-to-point optics is realized between the entrance and exit of QQDQQ magnets for the beam spectrometer. Fine-pitch wire chamber trackers and hodoscope counters are installed in the beam spectrometer to accept a high rate beam up to 107 Hz. The superconducting kaon spectrometer for scattered particles was transferred from KEK with modifications to the cryogenic system and detectors. A missing-mass resolution of 1.9 ± 0.1 MeV/c2 (FWHM) was achieved for the ∑ peaks of (π±, K+) reactions on a proton target in the first physics run of E19 in 2010

In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice

We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by headplate fixation and cranial window implantation. This protocol can be used for measuring neural activity and is suitable for long-term imaging in large populations. Moreover, this protocol does not require preparation of Flp-expressing transgenic mice

Forskolin stimulates porcine sperm capacitation by increasing calcium uptake

AbstractUsing the fluorescent calcium indicator fura-2, forskolin was found to dose-dependently cause an immediate increase in the concentration of intracellular free calcium of porcine cauda epididymal sperm. This stimulatory effect of forskolin is due to the enhancement of Ca2+ uptake by the verapamil-sensitive transporter on the sperm plasma membrane and results in the promotion of the sperm capacitation and subsequent acrosome reaction