学会抄録

Genome annotation. (XLSX 9 kb

Transcriptome-wide selection of a reliable set of reference genes for gene expression studies in potato cyst nematodes (<i>Globodera</i> spp.) - Table 2

<p>Comparison of rankings of 11 candidate reference genes from (A) RNA-Seq data and (B) qRT-PCR data, given by RefFinder in <i>G</i>. <i>rostochiensis</i>.</p

Variability of the expression level (span of Cq values) in <i>G. rostochiensis</i> of the 11 candidate reference genes across eight combinations of development stages and time of exposure to potato root diffusate, as measured by qRT-PCR.

<p>The median of three replicates of the eight conditions is represented by the line inside the box, 50% of the values are inside the box, the upper and lower edges represent the 75th and 25th percentiles, respectively, and the circles represent outliers.</p

Transcriptome-wide selection of a reliable set of reference genes for gene expression studies in potato cyst nematodes (<i>Globodera</i> spp.) - Fig 3

<p>Expression of (A) <i>NEP-1</i>, (B) <i>cht-2</i>, and (C) <i>eng</i> assessed by qRT-PCR in dry cysts (DC), water hydrated eggs (W), hydrated eggs exposed to potato root diffusate (PRD) for 1 h, 8 h, 24 h, 48 h, and 7 d, and in J2 larvae. All expression levels were normalized using the geometric mean of <i>aaRS</i>, <i>PMP-3</i>, and <i>GR</i>, <i>our selected reference genes (in black) and Act-1 (in grey)</i>. Dry cyst was used as the calibrator for relative expression calculation. Error bars represent the standard error of the mean, and significant differences among treatments are indicated by different letters (Tukey’s test).</p

Summation of the comprehensive ranking values of gene stability given by RefFinder for RNA-Seq and RT-qPCR variation analyses for 11 candidate reference genes.

<p>Expression variations were calculated on two RNA-Seq replicates and three qRT-PCR replicates for the eight developmental stages of <i>G</i>. <i>rostochiensis</i>.</p

Detailed rankings of 11 candidate reference genes from qRT-PCR data, given by RefFinder in <i>G</i>. <i>pallida</i>.

<p>Detailed rankings of 11 candidate reference genes from qRT-PCR data, given by RefFinder in <i>G</i>. <i>pallida</i>.</p

Description of genes and primers used in this study.

<p>Description of genes and primers used in this study.</p

nGr.v1.0.iprscan.xml

inter pro scan as gzipped xm

nGr.v1.0.annotation+TE

GFF file with locations of all gene calls in the G. rostochiensis genome and all transposable element predictions

nGr.v1.0.iprscan.xml

interproscan as ts