Evidence for the intense exchange of MazG in marine cyanophages by horizontal gene transfer

Background: S-PM2 is a phage capable of infecting strains of unicellular cyanobacteria belonging to the genus Synechococcus. S-PM2, like other myoviruses infecting marine cyanobacteria, encodes a number of bacterial-like genes. Amongst these genes is one encoding a MazG homologue that is hypothesized to be involved in the adaption of the infected host for production of progeny phage. Methodology/Principal Findings: This study focuses on establishing the occurrence of mazG homologues in other cyanophages isolated from different oceanic locations. Degenerate PCR primers were designed using the mazG gene of S-PM2. The mazG gene was found to be widely distributed and highly conserved among Synechococcus myoviruses and podoviruses from diverse oceanic provinces. Conclusions/Significance: This study provides evidence of a globally connected cyanophage gene pool, the cyanophage mazG gene having a small effective population size indicative of rapid lateral gene transfer despite being present in a substantial fraction of cyanophage. The Prochlorococcus and Synechococcus phage mazG genes do not cluster with the host mazG gene, suggesting that their primary hosts are not the source of the mazG gene

Data report: A downhole electrical resistivity study of northern Cascadia marine gas hydrate

Downhole electrical resistivity measurements can be exploited for gas hydrate concentration estimates. However, to do so requires that several assumptions be made, in particular about in situ pore water salinity and porosity. During Integrated Ocean Drilling Program Expedition 311, electrical resistivity was measured in four boreholes along a transect across the northern Cascadia margin, offshore Vancouver Island, Canada. Logging-while-rilling and conventional wireline logging data of resistivity, density, and neutron-porosity are used together with measurements of porosity and pore water salinity of the recovered core to systematically estimate gas hydrate concentrations at these four sites. Using Ar- chie’s law, empirical parameters a and m are determined from gas hydrate–free zones by means of a Pickett plot. The in situ salinity baseline trend for each site was estimated directly from the measured pore water salinity values, as well as indirectly by calculating the trend using Archie’s law and simultaneously solving for gas hydrate saturation and in situ salinity. Results showed that the in situ salinities at Sites U1325, U1326, and U1329 were well determined from a smooth trend through the highest measured salinity values of the recovered core. Only Site U1327 exhibitsstrongly decreasing pore water salinity, reaching 22‰ at the base of the gas hydrate stability field. This regional low salinity requires special analyses to estimate gas hydrate concentration from resistivity and introduces a large uncertainty. It is suggested that the decreased salinity results largely (90%) from a deeper fresh water source with the remaining freshening being the result of dissociation of pervasive gas hydrate (~3% of the pore space). Considering estimates from density porosity to be the most accurate, gas hydrate saturations average ~9% ± 7% at Site U1326, ~10% ± 7% at Site U1325, and 11% ± 7% at Site U1327 over the entire range of gas hydrate occurrence. No significant gas hydrate is inferred at Site U1329, although small am ounts may be present just above the bottom-simulating reflector. In two localized zones at Site U1326 (60–100 mbsf [meters below seafloor]) and Site U1327 (120–150 mbsf), significantly higher gas hydrate concentrations of >30% of the pore space were encountered

Wide angle seismic recordings from the 2002 Georgia Basin Geohazards Initiative, Northwestern Washington and British Columbia

This report describes the acquisition and processing of shallow-crustal wide-angle seismicreflection and refraction data obtained during a collaborative study in the Georgia Strait, western Washington and southwestern British Columbia. The study, the 2002 Georgia Strait Geohazards Initiative, was conducted in May 2002 by the Pacific Geoscience Centre, the U.S. Geological Survey, and the University of Victoria. The wide-angle recordings were designed to image shallow crustal faults and Cenozoic sedimentary basins crossing the International Border in southern Georgia basin and to add to existing wide-angle recordings there made during the 1998 SHIPS experiment. We recorded, at wide-angle, 800 km of shallow penetration multichannel seismic-reflection profiles acquired by the Canadian Coast Guard Ship (CCGS) Tully using an air gun with a volume of 1.967 liters (120 cu. in.). Prior to this reflection survey, we deployed 48 Refteks onshore to record the airgun signals at wide offsets. Three components of an oriented, 4.5 Hz seismometer were digitally recorded at all stations. Nearly 160,300 individual air gun shots were recorded along 180 short seismic reflection lines. In this report, we illustrate the wide-angle profiles acquired using the CCGS Tully, describe the land recording of the air gun signals, and summarize the processing of the land recorder data into common-receiver gathers. We also describe the format and content of the archival tapes containing the SEGY-formated, common-receiver gathers for the Reftek data. Data quality is variable but the experiment provided useful data from 42 of the 48 stations deployed. Three-fourths of all stations yielded useful first-arrivals to source-receiver offsets beyond 10 km: the average maximum source-receiver offset for first arrivals was 17 km. Six stations yielded no useful data and useful firstarrivals were limited to offsets less than 10 km at five stations. We separately archived our recordings of 86 local and regional earthquakes ranging in magnitude from 0.2 to 4.3 and 16 teleseisms ranging in magnitude 5.5 to 6.5

Gas hydrate on the northern Cascadia margin: regional geophysics and structural framework

Integrated Ocean Drilling Program Expedition 311 is based on extensive site survey data and historic research at the northern Cascadia margin since 1985. This research includes various regional geophysical surveys using a broad spectrum of seismic techniques, coring and logging by the Ocean Drilling Program Leg 146, heat flow measurements, shallow piston coring, and bottom video observations across a cold-vent field, as well as novel controlled-source electromagnetic and seafloor compliance surveying techniques. The wealth of data available allowed construction of structural cross-sections of the margin, development of models for the formation of gas hydrate in an accretionary prism, and estimation of gas hydrate and free gas concentrations. Expedition 311 established for the first time a transect of drill sites across the northern Cascadia margin to study the evolution of gas hydrate formation over the entire gas hydrate stability field of the accretionary complex. This paper reviews the tectonic framework at the northern Cascadia margin and summarizes the scientific studies that led to the drilling objectives of Expedition 311 Cascadia gas hydrate

Nested Case–Control Study of Autoimmune Disease in an Asbestos-Exposed Population

OBJECTIVE: To explore the potential association between asbestos exposure and risk of autoimmune disease, we conducted a case–control study among a cohort of 7,307 current and former residents of Libby, Montana, a community with historical occupational and environmental exposure to asbestos-contaminated vermiculite. METHODS: Cases were defined as those who reported having one of three systemic autoimmune diseases (SAIDs): systemic lupus erythematosus, scleroderma, or rheumatoid arthritis (RA). Controls were randomly selected at a 3:1 ratio from among the remaining 6,813 screening participants using frequency-matched age and sex groupings. RESULTS: The odds ratios (ORs) and 95% confidence intervals (CIs) for SAIDs among those ≥ 65 years of age who had worked for the vermiculite mining company were 2.14 (95% CI, 0.90–5.10) for all SAIDs and 3.23 (95% CI, 1.31–7.96) for RA. In this age group, exposure to asbestos while in the military was also an independent risk factor, resulting in a tripling in risk. Other measures of occupational exposure to vermiculite indicated 54% and 65% increased risk for SAIDs and RA, respectively. Those who had reported frequent contact with vermiculite through various exposure pathways also demonstrated elevated risk for SAIDs and RA. We found increasing risk estimates for SAIDs with increasing numbers of reported vermiculite exposure pathways (p < 0.001). CONCLUSION: These preliminary findings support the hypothesis that asbestos exposure is associated with autoimmune disease. Refined measurements of asbestos exposure and SAID status among this cohort will help to further clarify the relationship between these variables

Increasing Employee Awareness of the Signs and Symptoms of Heart Attack and the Need to Use 911 in a State Health Department

INTRODUCTION: Early recognition of the signs and symptoms of a heart attack can lead to reduced morbidity and mortality. METHODS: A workplace intervention was conducted among 523 Montana state health department employees in 2003 to increase awareness of the signs and symptoms of heart attack and the need to use 911. All employees received an Act in Time to Heart Attack Signs brochure and wallet card with their paychecks. Act in Time posters were placed in key workplace areas. A weekly e-mail message, including a contest entry opportunity addressing the signs and symptoms of heart attack, was sent to all employees. Baseline and follow-up telephone surveys were conducted to evaluate intervention effectiveness. RESULTS: Awareness of heart attack signs and symptoms and the need to call 911 increased significantly among employees from baseline to follow-up: pain or discomfort in the jaw, neck, or back (awareness increased from 69% to 91%); feeling weak, light-headed, or faint (awareness increased from 79% to 89%); call 911 if someone is having a heart attack or stroke (awareness increased from 84% to 90%). Awareness of chest pain, pain or discomfort in the arms or shoulders, and shortness of breath were more than 90% at baseline and did not increase significantly at follow-up. At baseline, 69% of respondents correctly reported five or more of the signs and symptoms of heart attack; 89% reported correctly at follow-up. CONCLUSION: This low-cost workplace intervention increased awareness of the signs and symptoms of heart attack and the need to call 911