List of SNPs

This file includes a list of all 10,497 SNPs along with their RAD tag IDs, allele sequences, and SNP position. See ReadMe tab for further details

Heat_Shock_Gene_Expression

Normalized gene expression values for six heat shock genes for liver tissues from heat stressed strains of O. mykiss gairdern

PCR analysis of plasmids in strains B31-A and B31-A3.

<p>Twenty-one pairs of specific primers were used to amplify all the circular and linear plasmids contained in the two strains. DNA markers (left lane) identify the sizes of amplified DNAs. “*” indicates the plasmids that are lost from B31-A strain. A) B31-A is missing lp25, lp28-1, lp28-4, lp36, cp32-6, cp32-7, lp5 and lp21, in addition to cp9. B) B31-A3 retains all twenty one plasmids except cp9, as expected <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004101#pone.0004101-Elias1" target="_blank">[22]</a>.</p

Effects of B31-A3, B31-A spent medium and BSK-II control medium on Lp.

<p>Relative changes in Lp are presented as Lp_test/Lp_control. Arrows indicate times at which perfusion with test solutions were initiated. A) One representative experiment showing a transient increase in Lp induced by B31-A3 spent medium. B) A representative experiment showing Lp is unaffected when vessels were perfused with BSK-II medium. C) Time course and magnitude changes in Lp from one representative paired experiment in which a venule was sequentially perfused with BSK-II medium, B31-A spent medium, BSK-II medium, then B31-A3 spent medium. An increase in Lp was only seen upon perfusion with B31-A3 spent medium. D) Summarized data of Lp changes induced by B31-A and B31-A3 spent medium; * indicates a significant increase (P<0.05) from negative control; † indicates a significant decrease (P<0.05) from B31-A3 spent medium.</p

SNP_Genotypes_PLINKinput.ped

SNP genotypes from survivors and mortalities of O. mykiss gairdneri under thermal stress

Effects of <i>Bb</i> spent medium on endothelial [Ca²⁺]_i.

<p>A) A representative venule was sequentially perfused with 5% albumin-Ringer (5% BSA Ringer), BSK-II medium, B31-A3 spent medium and 5% albumin-Ringer, showing no endothelial [Ca²⁺]_i changes upon exposure to BSK-II medium, while a transient increase in endothelial [Ca²⁺]_i was observed within 2–3 min after exposure to B31-A3 spent medium. B) Changes in endothelial [Ca²⁺]_i from one representative paired experiment, showing no significant changes in endothelial [Ca²⁺]_i during perfusion of B31-A spent medium, while a transient increase in endothelial [Ca²⁺]_i occurred upon perfusion of B31-A3 spent medium. Arrows indicate times at which perfusion with test solutions were initiated. C) Summarized data for the effects of spent medium from B31-A3 and B31-A on endothelial [Ca²⁺]_i; * indicates a significant increase (P<0.05) from negative control; † indicates a significant decrease (P<0.05) from B31-A3 spent medium.</p

Hydraulic conductivity, Lp, was measured based on the modified Landis technique in individually perfused microvessels.

<p>Jv is the initial water flow per unit area of microvessel wall (S), which was calculated from the velocity of the marker cell (Vcell) after the vessel was occluded, the vessel radius (r), and the length between the marker cell position and the occlusion site (L).</p

Measurements of changes in endothelial [Ca²⁺]_i in an individually perfused microvessel.

<p>The vessel was first perfused with albumin-Ringer solution containing Fura 2-AM for 45 min to load the endothelial cells of the microvessel wall. The vessel was then recannulated and perfused with albumin-Ringer solution for 10 min to remove fura 2-AM from the vessel lumen. Fluorescence intensity was collected through a rectangular diaphragm of the photometer (yellow area) under control conditions and after exposure to testing solutions.</p

Subsample of data showing PC analysis, admixture and F_ST results for only individuals identified as the proposed Inland Roach species.

<p>Initial analyses (A–C) show results which include individuals sampled from the Red Hills region, a proposed subspecies of Inland Roach. Subsequent analyses (C–E) exclude these Red Hills individuals. Colors are consistent across all subfigures and nomenclature is consistent with abbreviations in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0189417#pone.0189417.s007" target="_blank">S1 Table</a>.</p

Datasheet1_Perceived potentially inappropriate treatment in the PICU: frequency, contributing factors and the distress it triggers.docx

BackgroundPotentially inappropriate treatment in critically ill adults is associated with healthcare provider distress and burnout. Knowledge regarding perceived potentially inappropriate treatment amongst pediatric healthcare providers is limited.ObjectivesDetermine the frequency and factors associated with potentially inappropriate treatment in critically ill children as perceived by providers, and describe the factors that providers report contribute to the distress they experience when providing treatment perceived as potentially inappropriate.MethodsProspective observational mixed-methods study in a single tertiary level PICU conducted between March 2 and September 14, 2018. Patients 0–17 years inclusive with: (1) ≥1 organ system dysfunction (2) moderate to severe mental and physical disabilities, or (3) baseline dependence on medical technology were enrolled if they remained admitted to the PICU for ≥48 h, and were not medically fit for transfer/discharge. The frequency of perceived potentially inappropriate treatment was stratified into three groups based on degree of consensus (1, 2 or 3 providers) regarding the appropriateness of ongoing active treatment per enrolled patient. Distress was self-reported using a 100-point scale.ResultsOf 374 patients admitted during the study, 133 satisfied the inclusion-exclusion criteria. Eighteen patients (unanimous - 3 patients, 2 providers - 7 patients; single provider - 8 patients) were perceived as receiving potentially inappropriate treatment; unanimous consensus was associated with 100% mortality on 3-month follow up post PICU discharge. Fifty-three percent of providers experienced distress secondary to providing treatment perceived as potentially inappropriate. Qualitative thematic analysis revealed five themes regarding factors associated with provider distress: (1) suffering including a sense of causing harm, (2) conflict, (3) quality of life, (4) resource utilization, and (5) uncertainty.ConclusionsWhile treatment perceived as potentially inappropriate was infrequent, provider distress was commonly observed. By identifying specific factor(s) contributing to perceived potentially inappropriate treatment and any associated provider distress, organizations can design, implement and assess targeted interventions.</p