Missed, not missing: Phylogenomic evidence for the existence of Avian FoxP3

The Forkhead box transcription factor FoxP3 is pivotal to the development and function of regulatory T cells (Tregs), which make a major contribution to peripheral tolerance. FoxP3 is believed to perform a regulatory role in all the vertebrate species in which it has been detected. The prevailing view is that FoxP3 is absent in birds and that avian Tregs rely on alternative developmental and suppressive pathways. Prompted by the automated annotation of foxp3 in the ground tit (Parus humilis) genome, we have questioned this assumption. Our analysis of all available avian genomes has revealed that the foxp3 locus is missing, incomplete or of poor quality in the relevant genomic assemblies for nearly all avian species. Nevertheless, in two species, the peregrine falcon (Falco peregrinus) and the saker falcon (F. cherrug), there is compelling evidence for the existence of exons showing synteny with foxp3 in the ground tit. A broader phylogenomic analysis has shown that FoxP3 sequences from these three species are similar to crocodilian sequences, the closest living relatives of birds. In both birds and crocodilians, we have also identified a highly proline-enriched region at the N terminus of FoxP3, a region previously identified only in mammals

Effect of Preoperative Corticosteroids on Postoperative Glucose Control in Total Joint Arthroplasty

Background: Dexamethasone is a commonly used perioperative medication for the management of postoperative nausea and vomiting following total joint arthroplasty (TJA). However, concerns have been raised about its potential to cause hyperglycemia. This study aimed to investigate the impact of dexamethasone administration on glucose levels and complications in both diabetic and nondiabetic patients undergoing TJA. Methods: We performed a retrospective review of 1928 patients who underwent primary total knee and hip arthroplasty procedures at a large tertiary medical institution. Patients were divided into 2 groups based on whether they received preoperative dexamethasone. Postoperative blood glucose values and variability were measured, and data on complications were collected. We performed statistical analysis using descriptive analysis, multivariate logistic regression models, negative binomial regression, and a subset analysis to assess the impact of dexamethasone dose on postoperative glycemic control. Results: Preoperative dexamethasone did not significantly increase the mean glucose, fasting glucose, glucose variability, or 90-day complications in both the diabetic and nondiabetic groups. Nondiabetic patients who received dexamethasone had a significantly lower rate of intensive care unit admission (P < .01). Additionally, patients who received dexamethasone had a significantly shorter length of hospital stay compared to those who did not (P < .001). Conclusions: Preoperative dexamethasone administration is a safe and effective method for preventing postoperative nausea and vomiting in patients undergoing TJA without significant adverse effects on glucose levels, glucose variability, or infection rates in both diabetics and nondiabetics. Preoperative dexamethasone decreases postoperative length of stay

The genomic context of avian <i>foxp3</i>.

<p>(A) The gene neighbourhood of <i>foxp3 in</i> a number of archosaurs showing the significant masked repeats of the American crow between <i>hsd17b10</i> and <i>cacna1f</i> (gaps in the line indicate intergenic repeat regions), the approximate location of the <i>foxp3</i> locus in the saker and peregrine falcon (marked by a pink arrow with a dashed line), and the low nucleotide sequence identity of <i>foxp3</i> between the American alligator and the ground tit. In the displayed region the overall masked repeats are as follows: 54.7% in the American crow, 28.0% in the saker falcon, 31.4% in the peregrine falcon, 6.8% in the ground tit and 0.02% in the American alligator. (B) The <i>foxp3</i> gene neighbourhood of the mouse, American alligator and ground tit, showing the gene variation upstream of <i>foxp3</i> in mammals, birds and crocodilians and the low nucleotide sequence identity of murine <i>foxp3</i> versus the American alligator and ground tit. (C) A comparison of the approximate <i>foxp3</i> loci in both falcons with the ground tit <i>foxp3 locus</i>, showing masked repeats (gaps in the line) and predicted exons (rectangles). All subfigures were produced using Easyfig [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150988#pone.0150988.ref047" target="_blank">47</a>] with BLASTN identity comparisons indicated by the scales on each subfigure.</p

Association of <i>FGF4L1</i> Retrogene Insertion with Prolapsed Gland of the Nictitans (Cherry Eye) in Dogs

Cherry eye is the common name for prolapse of the nictitans gland, a tear-producing gland situated under the third eyelid of dogs. Cherry eye is characterized by a red fleshy protuberance in the corner of the eye, resembling a cherry. This protrusion is a displacement of the normal gland of the third eyelid, thought to be caused by a defect in the connective tissue that secures the gland in place. Options for treatment may include anti-inflammatory medications in mild cases, but surgical replacement of the gland is usually indicated. Cherry eye is most often seen in dogs under the age of two years, with certain breeds having a higher incidence, suggesting a potential genetic association. Integration of panel genetic testing into routine clinical practice allows for the generation of large numbers of genotyped individuals paired with clinical records and enables the investigation of common disorders using a genome-wide association study (GWAS) approach at scale. In this investigation, several thousand cases and controls for cherry eye in both purebred dogs and mixed breeds are used for a large-scale GWAS, revealing a single peak of genome-wide significance on canine chromosome 18, directly at the location of the previously identified FGF4 insertion known to cause chondrodysplasia in several breeds

The genomic conservation of <i>foxp3</i> with its downstream flanking genes, <i>cacna1f</i> and <i>ccdc22</i>.

<p>The genomic conservation of <i>foxp3</i> with its downstream flanking genes, <i>cacna1f</i> and <i>ccdc22</i>.</p

Avian genome quality and its relationship to the annotation and assembly of the <i>foxp3</i> gene neighbourhood.

<p>Avian genome quality and its relationship to the annotation and assembly of the <i>foxp3</i> gene neighbourhood.</p

Alignment of the forkhead domain of FoxP3 and FoxP3-like proteins, highlighting mammalian and avian signature residues.

<p>The consistent exon boundaries in the ground tit and peregrine falcon were used to curate the gap within the sequence of the saker falcon. The mouse sequence is used as the baseline sequence and only amino acids in other sequences that differ are shown.</p

Ground tit <i>foxp3</i> transcriptome coverage.

<p>The 11 numbered exons of the putative ground tit <i>foxp3</i> sequence annotated with the sequence regions that encode ground tit FoxP3 functional regions. A collection of reads with 100% identity (shown in green) were found with a BLASTN search of ground tit RNA-Seq data from the muscle in the SRA (SRX246872; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150988#pone.0150988.s002" target="_blank">S2 File</a>).</p

A summary of the structural domains and regions of FoxP3 in different clades of the Animal Kingdom.

<p>A summary of the structural domains and regions of FoxP3 in different clades of the Animal Kingdom.</p

The distinct forkhead domain of FoxP3.

<p>The FoxP3 forkhead domain of the mouse, alligator and ground tit, aligned to FoxP1, FoxP2 and FoxP4 paralogues in the same species, demonstrating its divergent evolution and unique signature residues. The mouse FoxP3 sequence is used as the baseline sequence and only amino acids in other sequences that differ are shown.</p