Lysine mediation of neuroendocrine food regulation in guinea fowl

In poultry, obesity is partly influenced by food intake, and is increasingly becoming a nationwide problem. Hypothalamic food intake mechanisms are involved metabolically and neurologically via two peptide hormones, leptin and ghrelin, and the amino acid glutamate, which is enzymatically derived from lysine metabolism. We hypothesize that lysine homeostasis mediates regulation of feed intake and performance characteristics via the brain–liver axis through glutamate sensing. The objective was to examine the effects of lysine homeostasis in avian food regulation and performance through neuroendocrine signaling. One-day-old male French Guinea fowl (GF) keets (n = 270) were weighed and randomly assigned to 5 dietary treatments (0.80%, 0.86%, 0.92%, 1.10% control, and 1.22% lysine) in 3 replicates. At 4 and 8 wk of age 20% of experimental birds were randomly selected, weighed and euthanatized. The liver, pancreas, and hypothalamus were excised, snap frozen in liquid nitrogen and stored at –80°C until use. Tissue mRNA was extracted and cDNA synthesized for qPCR assays. Lysine at 0.80 and 0.86% hindered growth, development of digestive organs, expression of brain and liver glutamate and leptin receptors, and caused high mortality in GF. The fold change for metabotropic glutamate receptor I was lower (P \u3c 0.05) in liver and higher in brain at 0.86 and 0.92% than the control (1.10%) and 1.22% lysine. The 1.22% lysine exhibited highest expression of ionotropic glutamate receptor, while brain ghrelin receptor expression was highest at 0.86 and 0.92% lysine. Therefore, dietary lysine concentration may influence signaling pathways regulating food intake in brain-liver axis via glutamate synthesis

Conservation of Wild-harvested MedicinalPlant Species in Tanzania : Chain and consequence of commercial trade on medicinal plant species

Tanzania is endowed with more than 10,000 plant species, of which 1,100 are endemic. The coastal regions host most endemic species, due to its wide range of productive ecological conditions. Over 25 % of all species are used as wild-harvested medicinal plants. About 60% of the Tanzanian population in both rural and urban areas depends on traditional medicine and herbs as their primary health care, and as a means of generating income. This is due to high costs and unavailability of the universal healthcare, which was abolished in 1993. The aim of the thesis is to make a structured and a quantitative investigation to identify traded medicinal plants traded in markets of Dar es Salaam in Tanzania with a purpose to understand the chain and consequences of commercial trade on wild-harvested medicinal plants. A quantitative market research using free list surveys was used in combination with individually repeated in-depth structured and semi-structured interviews. Furthermore, Conservation Assessment and Planning Management method was used to prioritise species that are in need of conservation due to commercial trade. The results show that the chain of commercial trade of medicinal plant species begins in the forest and is then distributed to different stakeholders, such as harvesters, vendors, Traditional Healing Practitioners, consumers and exporters. The research also shows that the large urban and international demand of medicinal plant species supplied by the vendors and exporters is the major threat, specifically to destructively harvested species such as Cassia abbreviata, Zanthoxylum usambarense, Zanthoxylum chalybeum, Myrsine africana, Milicia excelsa and Prunus Africana. These medicinal plants species are up-rooted and/or ring-barked and are therefore in need of conservation. This calls for several conservation guidelines such as policy and regulations, Traditional Ecological Knowledge, education and planting of the medicinal plant species in their natural forests as well as collecting samples of medicinal plant species in a herbarium. Policies can be implemented to conserve these species, for instance only allowing registered practitioners to harvest the medicinal plant species to a level that will sustainably balance the quantity of medicinal plant species in the forest and its supply. In conclusion, an important policy regulation could be an export tax on internationally-traded wild-harvested medicinal that is levied on the purchasing international pharmaceutical companies, which would then be used to fund the conservation of the medicinal plant species to retain a sustainable wild stock. More investigation on the actual population sizes of these plant species is needed in order to secure their existence and contribute to sustainable development both socially and environmentall

Conservation of Wild-harvested MedicinalPlant Species in Tanzania : Chain and consequence of commercial trade on medicinal plant species

Tanzania is endowed with more than 10,000 plant species, of which 1,100 are endemic. The coastal regions host most endemic species, due to its wide range of productive ecological conditions. Over 25 % of all species are used as wild-harvested medicinal plants. About 60% of the Tanzanian population in both rural and urban areas depends on traditional medicine and herbs as their primary health care, and as a means of generating income. This is due to high costs and unavailability of the universal healthcare, which was abolished in 1993. The aim of the thesis is to make a structured and a quantitative investigation to identify traded medicinal plants traded in markets of Dar es Salaam in Tanzania with a purpose to understand the chain and consequences of commercial trade on wild-harvested medicinal plants. A quantitative market research using free list surveys was used in combination with individually repeated in-depth structured and semi-structured interviews. Furthermore, Conservation Assessment and Planning Management method was used to prioritise species that are in need of conservation due to commercial trade. The results show that the chain of commercial trade of medicinal plant species begins in the forest and is then distributed to different stakeholders, such as harvesters, vendors, Traditional Healing Practitioners, consumers and exporters. The research also shows that the large urban and international demand of medicinal plant species supplied by the vendors and exporters is the major threat, specifically to destructively harvested species such as Cassia abbreviata, Zanthoxylum usambarense, Zanthoxylum chalybeum, Myrsine africana, Milicia excelsa and Prunus Africana. These medicinal plants species are up-rooted and/or ring-barked and are therefore in need of conservation. This calls for several conservation guidelines such as policy and regulations, Traditional Ecological Knowledge, education and planting of the medicinal plant species in their natural forests as well as collecting samples of medicinal plant species in a herbarium. Policies can be implemented to conserve these species, for instance only allowing registered practitioners to harvest the medicinal plant species to a level that will sustainably balance the quantity of medicinal plant species in the forest and its supply. In conclusion, an important policy regulation could be an export tax on internationally-traded wild-harvested medicinal that is levied on the purchasing international pharmaceutical companies, which would then be used to fund the conservation of the medicinal plant species to retain a sustainable wild stock. More investigation on the actual population sizes of these plant species is needed in order to secure their existence and contribute to sustainable development both socially and environmentall

Conservation of Wild-harvested MedicinalPlant Species in Tanzania : Chain and consequence of commercial trade on medicinal plant species

Tanzania is endowed with more than 10,000 plant species, of which 1,100 are endemic. The coastal regions host most endemic species, due to its wide range of productive ecological conditions. Over 25 % of all species are used as wild-harvested medicinal plants. About 60% of the Tanzanian population in both rural and urban areas depends on traditional medicine and herbs as their primary health care, and as a means of generating income. This is due to high costs and unavailability of the universal healthcare, which was abolished in 1993. The aim of the thesis is to make a structured and a quantitative investigation to identify traded medicinal plants traded in markets of Dar es Salaam in Tanzania with a purpose to understand the chain and consequences of commercial trade on wild-harvested medicinal plants. A quantitative market research using free list surveys was used in combination with individually repeated in-depth structured and semi-structured interviews. Furthermore, Conservation Assessment and Planning Management method was used to prioritise species that are in need of conservation due to commercial trade. The results show that the chain of commercial trade of medicinal plant species begins in the forest and is then distributed to different stakeholders, such as harvesters, vendors, Traditional Healing Practitioners, consumers and exporters. The research also shows that the large urban and international demand of medicinal plant species supplied by the vendors and exporters is the major threat, specifically to destructively harvested species such as Cassia abbreviata, Zanthoxylum usambarense, Zanthoxylum chalybeum, Myrsine africana, Milicia excelsa and Prunus Africana. These medicinal plants species are up-rooted and/or ring-barked and are therefore in need of conservation. This calls for several conservation guidelines such as policy and regulations, Traditional Ecological Knowledge, education and planting of the medicinal plant species in their natural forests as well as collecting samples of medicinal plant species in a herbarium. Policies can be implemented to conserve these species, for instance only allowing registered practitioners to harvest the medicinal plant species to a level that will sustainably balance the quantity of medicinal plant species in the forest and its supply. In conclusion, an important policy regulation could be an export tax on internationally-traded wild-harvested medicinal that is levied on the purchasing international pharmaceutical companies, which would then be used to fund the conservation of the medicinal plant species to retain a sustainable wild stock. More investigation on the actual population sizes of these plant species is needed in order to secure their existence and contribute to sustainable development both socially and environmentall

Process Modelling of Geothermal Drilling System Using Digital Twin for Real-Time Monitoring and Control

Currently, Kenya supplies its energy demand predominantly through hydroelectric power, which fluctuates due to poor and unpredictable rainfall in particular years. Geothermal energy is proposed as a clean and reliable energy source in meeting Kenya’s increasing energy demand. During geothermal drilling operations, disruptions due to tool wear and breakages increases the cost of operation significantly. Some of these causes can be mitigated by real-time monitoring of the tool head during operations. This paper presents the design and implementation of a digital twin model of a drilling tool head, represented as a section of a mechatronic assembly system. The system was modelled in Siemens NX and programmed via the TIA portal using S7 1200 PLC. The digital model was programmed to exactly match the operations of the physical system using OPC (open platform communications) standards. These operations were verified through the motion study by simultaneous running of the assembly system and digital twin model. The study results substantiate that a digital twin model of a geothermal drilling operation can closely mimic the physical operation