Initial sequencing and analysis of the human genome

The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and make freely available a draft sequence of the human genome. We also present an initial analysis of the data, describing some of the insights that can be gleaned from the sequence.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/62798/1/409860a0.pd

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Spatio-temporal dynamics of land use changes in response to external pressures in Oman: Greenhouse cropping as an example

Further from the northern coast of Oman new farm developments were more frequent than closer to the coast; they were also larger. The density of farms was highest close to Muscat although the distance enclosing 50% of farms had shifted away from Muscat during the study period. The dominance of Muscat is likely to be related to access to markets and infrastructure development. The increase in groundwater salinity was also highest close to Muscat and may be responsible for the shift in greenhouse density. Salinization of groundwater is severe close to the coast and was responsible for the reduced density of greenhouses near the coast. Land abandonment was highest close to Muscat and to the coast, reflecting changes in groundwater salinity and urbanization pressure. Less evidence was available for a direct shift from farmland to urban land use. Recent urban developments were largely located in areas already abandoned by agriculture. The paper also discusses likely future trends in land use change given that Oman’s population is increasing at over 2% annually and demand for urban land is increasing. The identification of a “salinity corridor” within which much of the future land use change may occur is discussed

Spatio-temporal dynamics of land use changes in response to external pressures in Oman: Greenhouse cropping as an example

Further from the northern coast of Oman new farm developments were more frequent than closer to the coast; they were also larger. The density of farms was highest close to Muscat although the distance enclosing 50% of farms had shifted away from Muscat during the study period. The dominance of Muscat is likely to be related to access to markets and infrastructure development. The increase in groundwater salinity was also highest close to Muscat and may be responsible for the shift in greenhouse density. Salinization of groundwater is severe close to the coast and was responsible for the reduced density of greenhouses near the coast. Land abandonment was highest close to Muscat and to the coast, reflecting changes in groundwater salinity and urbanization pressure. Less evidence was available for a direct shift from farmland to urban land use. Recent urban developments were largely located in areas already abandoned by agriculture. The paper also discusses likely future trends in land use change given that Oman’s population is increasing at over 2% annually and demand for urban land is increasing. The identification of a “salinity corridor” within which much of the future land use change may occur is discussed

Evaluation of Warm Season Turfgrass under Different Irrigation Regimes in Arid Region

Turfgrasses play a very important role in enhancing quality of life in modern urban living. Water quantity is the most important challenge worldwide in establishing and maintaining quality turf. The present study was aimed to test the performance of three warm season turfgrasses under four water levels for plantation in arid zones. Pits (48) measuring 1m length x 1m width x 0.6 m depth were planted with four replications of Common Bermuda grass (Cynodon dactylon), Tifway Bermuda grass (Cynodon dactylon x transvaalensis) and Seashore Paspalum grass (Paspalum vaginatum) in complete randomized design (CRD). Irrigation was done daily with 15 l/plot during the first 4 weeks (establishment period) and four irrigation levels (5, 10, and 15, 20 l/lot) were maintained in the following 8 weeks (treatment period). Physical parameters (canopy temperatures, ambient temperature, leaf area, shoot production and relative water content) were measured once in two week as well as the visual quality (shoot color, shoot density and shoot uniformity) was assessed, however, chlorophyll analysis was done in the end of the study. It was found that temperature has significant effect on performance of turfgrasses. Canopy temperature was higher than ambient temperature in the three turfgrasses but it has different level in each variety. Five liter of water per day per square meter gave acceptable turf quality when ambient temperature ranged from 20 to 33�C. Seashore paspalum performed best followed by Tifway Bermuda grass and common Bermuda grass respectively

Potential for Transcranial Laser or LED Therapy to Treat Stroke, Traumatic Brain Injury, and Neurodegenerative Disease

Near-infrared (NIR) light passes readily through the scalp and skull and a small percentage of incident power density can arrive at the cortical surface in humans.1 The primary photoreceptors for red and NIR light are mitochondria, and cortical neurons are exceptionally rich in mitochondria. It is likely that brain cells are ideally set up to respond to light therapy. The basic biochemical pathways activated by NIR light, e.g., increased adenosine-5’-triphosphate (ATP) production, and signaling pathways activated by reactive oxygen species, nitric oxide release, and increased cyclic adenosine monophosphate (AMP) all work together to produce beneficial effects in brains whose function has been compromised by ischemia, traumatic injury, or neurodegeneration. One of the main mechanisms of action of transcranial light therapy (TLT) is to prevent neurons from dying, when they have been subjected to some sort of hypoxic, traumatic, or toxic insult. This is probably because of light-mediated upregulation of cytoprotective gene products such as antioxidant enzymes, heat shock proteins, and anti-apoptotic proteins. Light therapy in vitro has been shown to protect neurons from death caused by methanol,2 cyanide or tetrodotoxin, 3 and amyloid beta peptide.4 There is also probably a second mechanism operating in TLT; increased neurogenesis. Neurogenesis is the generation of neuronal precursors and birth of new neural cells.5 Two key sites for adult neurogenesis include the subventricular zone (SVZ) of the lateral ventricles, and the subgranular layer (SGL) of the dentate gyrus in the hippocampus.6 Neurogenesis can be stimulated by physiological factors, such as growth factors and environmental enrichment, and by pathological processes, including ischemia and neurodegeneration.7 Adult neurogenesis (in the hippocampus particularly) is now recognized as a major determinant of brain function both in experimental animals and in humans. Neural progenitor cells in their niche in the SGL of the dentate gyrus give birth to newly formed neurons that are thought to play a role in brain function, particularly in olfaction and in hippocampal-dependent learning and memory. In small animal models neurogenesis can be readily detected by incorporation of bromodeoxyuridine (BrdU), injected before euthanasia, into proliferating brain cells. Increased neurogenesis after TLT, has been demonstrated in a rat model of stroke,9 and in the Hamblin laboratory after TLT for acute traumatic brain injury (TBI) in mice (W. Xuan, T. Ando, et al., unpublished data). These two mechanisms of action of TLT in ameliorating brain damage (prevention of neuronal death and increased neurogenesis) have motivated studies in both animals and humans for diverse brain disorders and diseases. TLT for acute stroke is the most developed,10 but acute TBI has also been shown to benefit from TLT.11 These areas are reviewed further.United States. Dept. of Veterans Affairs. Medical Research ServiceNational Institutes of Health (U.S.) (Grant R01AI50875)Center for Integration of Medicine and Innovative Technology (DAMD17-02-2-0006)United States. Dept. of Defense. Congressionally Directed Medical Research Programs ( Program in TBI W81XWH-09-1-0514)United States. Air Force Office of Scientific Research (F9950-04-1-0079

Effect of grafting on resistance to vine decline disease, yield and fruit quality in muskmelon cv. Sawadi

A study was conducted to evaluate graft success, resistance to vine decline disease and effect of grafting on quality and yield of muskmelon. Two field experiments, conducted in Oman, showed that muskmelon cv. Sawadi grafted on six cucurbit rootstocks gave high grafting success: 97.6-99.1% (avg. 98.6%) and 92.4-96.9% (avg. 95.3%) under field conditions in fall 2012 and spring 2013 respectively. No significant differences were observed between seasons among the six treatments and a non-grafted control in consumer preference for odor and firmness, fruit shape, flesh vitamin C, micronutrient content  and TSS (sucrose %) or pH in spring 2013 (P > 0.05). The concentration of phosphorus and sodium significantly decreased in both seasons in all treatments in comparison to the control (P > 0.05). In both seasons potassium content significantly increased when Rsscih7458 and Mubyeongjangsoo rootstocks were used (P < 0.05). Strong Tosa rootstocks showed zero graft failure, high resistance to vine decline disease, high yield production and higher TSS (sucrose %) as compared to other rootstocks. Strong Tosa and Tetsukabuto rootstocks showed significantly higher consumer acceptance for rind color, flesh color and overall consumer acceptance in spring 2013 and was also less affected by seasonal changes. Results suggested that by grafting muskmelon cv. Sawadi some quality attributes may be improved in addition to the increased level of resistance to disease. However, additional trials are required to make final recommendations for the farming community