Longitudinal Monitoring of SARS-CoV-2 IgM and IgG Seropositivity to Detect COVID-19.

BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a novel beta-coronavirus that has recently emerged as the cause of the 2019 coronavirus pandemic (COVID-19). Polymerase chain reaction (PCR) based tests are optimal and recommended for the diagnosis of an acute SARS-CoV-2 infection. Serology tests for viral antibodies provide an important tool to diagnose previous exposure to the virus. Here we evaluate the analytical performance parameters of the Diazyme SARS-CoV-2 IgM/IgG serology assays and describe the kinetics of IgM and IgG seroconversion observed in patients with PCR-confirmed COVID-19 who were admitted to our hospital.MethodsWe validated the performance of the Diazyme assay in 235 presumed SARS-CoV-2 negative subjects to determine specificity. Subsequently, we evaluated the SARS-CoV-2 IgM and IgG seroconversion of 54 PCR-confirmed COVID-19 patients and determined sensitivity of the assay at three different timeframes.ResultSensitivity and specificity for detecting seropositivity at ≥15 days following a positive SARS-CoV-2 PCR result, was 100.0% and 98.7% when assaying for the panel of IgM and IgG. The median time to seropositivity observed for a reactive IgM and IgG result from the date of a positive PCR was 5 days (IQR: 2.75-9 days) and 4 days (IQR: 2.75-6.75 days), respectively.ConclusionsOur data demonstrate that the Diazyme IgM/IgG assays are suited for the purpose of detecting SARS-CoV-2 IgG and IgM in patients with suspected SARS-CoV-2 infections. For the first time, we report longitudinal data showing the evolution of seroconversion for both IgG and IgM in a cohort of acutely ill patients in the United States. We also demonstrate a low false positive rate in patients who were presumed to be disease free

On the master equation approach to kinetic theory: linear and nonlinear Fokker--Planck equations

We discuss the relationship between kinetic equations of the Fokker-Planck type (two linear and one non-linear) and the Kolmogorov (a.k.a. master) equations of certain N-body diffusion processes, in the context of Kac's "propagation of chaos" limit. The linear Fokker-Planck equations are well-known, but here they are derived as a limit N->infty of a simple linear diffusion equation on (3N-C)-dimensional N-velocity spheres of radius sqrt(N) (with C=1 or 4 depending on whether the system conserves energy only or energy and momentum). In this case, a spectral gap separating the zero eigenvalue from the positive spectrum of the Laplacian remains as N->infty,so that the exponential approach to equilibrium of the master evolution is passed on to the limiting Fokker-Planck evolution in R^3. The non-linear Fokker-Planck equation is known as Landau's equation in the plasma physics literature. Its N-particle master equation, originally introduced (in the 1950s) by Balescu and Prigogine (BP), is studied here on the (3N-4)-dimensional N-velocity sphere. It is shown that the BP master equation represents a superposition of diffusion processes on certain two-dimensional sub-manifolds of R^{3N} determined by the conservation laws for two-particle collisions. The initial value problem for the BP master equation is proved to be well-posed and its solutions are shown to decay exponentially fast to equilibrium. However, the first non-zero eigenvalue of the BP operator is shown to vanish in the limit N->infty. This indicates that the exponentially fast approach to equilibrium may not be passed from the finite-N master equation on to Landau's nonlinear kinetic equation.Comment: 20 pages; based on talk at the 18th ICTT Conference. Some typos and a few minor technical fixes. Modified title slightl

Allantoin Crystal Formation in Bagrada hilaris (Burmeister) (Heteroptera: Pentatomidae) Females.

Bagrada hilaris is a polyphagous herbivore reported as an invasive pest in the United States. During the course of dissecting Burmeister hilaris unique crystals were observed in both the midgut and oviducts. Crystals were identified using X-ray diffraction techniques. Both acicular (i.e., needle-like, slender, and/or tapered) and cubic (i.e., cube shaped) crystals were observed in six of 75 individuals examined (8.0%). The crystals were mainly observed in females (6.7%), followed by males (1.3%) with no crystals observed in the minimal number of nymphs examined (0%). Crystals of both types were detected in the midgut and lateral oviducts of the females and midgut in males. The acicular crystals often appeared as distinct bundles when present in the midgut and oviducts. Crystals varied in size with the acicular crystals ranging from 0.12 mm to 0.5 mm in length although the cubic crystals ranged in length from 0.25 mm to over 1.0 mm with widths of ∼0.25 mm. The cubic crystals were identified as allantoin although the acicular crystals were most likely dl-allantoin in combination with halite. While allantoin in a soluble form is often found in insect tissues and excreta; being present as a crystal, especially in such a large form, is curious and raises some interesting questions. More research is warranted to further understand mechanisms associated with such crystal formation in B. hilaris and can lead to a better understanding of the excretory process in this species and the role allantoin plays in the elimination of excess nitrogen

Wave operator bounds for 1-dimensional Schr\"odinger operators with singular potentials and applications

Boundedness of wave operators for Schr\"odinger operators in one space dimension for a class of singular potentials, admitting finitely many Dirac delta distributions, is proved. Applications are presented to, for example, dispersive estimates and commutator bounds.Comment: 16 pages, 0 figure

Elevated corticosterone in feathers correlates with corticosterone-induced decreased feather quality: A validation study

The newly described technique of extracting corticosterone (CORT) from bird feathers may serve as a less invasive, more integrated measure of a bird\u27s stress response. Previous work indicated that elevated plasma CORT resulted in poorer quality feathers during molt. We tested the hypothesis that a direct link exists between plasma and feather CORT concentrations. We experimentally increased plasma CORT concentrations using implants and found that the corresponding rise in CORT could be detected in feathers grown during implantation. Furthermore, CORT levels in two feathers grown at the same time from the same bird were very consistent. These results provide evidence that elevated CORT is a causative factor in decreasing feather quality during molt. However, there remain technical details that suggest caution when interpreting data from CORT extracted from feathers. Different portions of a growing feather did not necessarily reflect changes in plasma CORT at the time different parts of the feather were forming, a standard pool of homogenized feathers indicated that sample mass affects measured feather CORT concentration, and different antibodies produced different measured CORT concentrations, leaving in doubt the exact steroid being assayed. © 2011 The Authors

Are receptor concentrations correlated across tissues within individuals? A case study examining glucocorticoid and mineralocorticoid receptor binding

Copyright © 2015 by the Endocrine Society. Hormone receptors are a necessary (although not sufficient) part of the process through which hormones like corticosterone create physiological responses. However, it is currently unknown to what extent receptor concentrations across different target tissues may be correlated within individual animals. In this study, we examined this question using a large dataset of radioligand binding data for glucocorticoid receptors (GRs) and mineralocorticoid receptors (MRs) in 13 different tissues in the house sparrow (Passer domesticus) (n = 72). Our data revealed that individual house sparrows tended to exhibit higher or lower receptor binding across all tissues, which could be part of what creates the physiological and behavioral syndromes associated with different hormonal profiles. However, although statistically significant, the correlations between tissues were very weak. Thus, when each tissue was independently regressed on receptor concentrations in the other tissues, multivariate analysis revealed significant relationships only for sc fat (for GR) and whole brain, hippocampus, kidney, omental fat, and sc fat (for MR). We also found significant pairwise correlations only between receptor concentrations in brain and hippocampus, and brain and kidney (both for MR). This research reveals that although there are generalized individual consistencies in GR and MR concentrations, possibly due to such factors as hormonal regulation and genetic effects, the ability of 2 different tissues to respond to the same hormonal signal appears to be affected by additional factors that remain to be identified

Calibrations of phase abundance, composition, and particle size distribution for olivine-orthopyroxene mixtures from reflectance spectra

Spectral reflectance measurements of characterized (phase abundance, particle size) mixtures of olivine and orthopyroxene were utilized to define the correlations between spectral and albedo parameters of such assemblages and their mineralogical or textural properties. Thirty-three different spectral parameters falling into three general classes (relative or ratioed, absolute or albedo, and wavelength) were investigated for empirical sensitivity to one or more of the mixture properties. Theoretical considerations and previous experimental observations were utilized to understand their functional relationships. The ratio of areas for the 1- and 2-3µm absorption bands is shown to be a sensitive indicator of the olivine-orthopyroxene abundance and is very nearly independent of particle size and mineral composition. In conjunction with an abundance determination, the wavelength position of the 1-3tm absorption feature can be utilized to determine the molar iron contents of the olivine and orthopyroxene phases. This calibration is insensitive to particle size but will produce systematic deviations if the phases have significantly different iron contents or if more than a few percent of a clinopyroxene component is present. The spectral albedo in the 0.6- to 0.7-µm region is relatively insensitive to phase abundance and can be used to constrain particle size if phase composition has been determinedVarious portions of this work were supported at the University of Hawaii by NASA grant NSG-7312 and by a Sigma Xi Foundation grant to E.A.C. and at Rensselaer Polytechnic Institute by NASA grant NAGW-642. Planetary Geosciences Division, Hawaii Institute of Geophysics, University of Hawaii, publication4 70

Population III star formation in a Lambda CDM universe, I: The effect of formation redshift and environment on protostellar accretion rate

(abridged) We perform 12 extremely high resolution adaptive mesh refinement cosmological hydrodynamic simulations of Population III star formation in a Lambda CDM universe, varying the box size and large-scale structure, to understand systematic effects in the formation of primordial protostellar cores. We find results that are qualitatively similar to those observed previously. We observe that the threshold halo mass for formation of a Population III protostar does not evolve significantly with time in the redshift range studied (33 > z > 19) but exhibits substantial scatter due to different halo assembly histories: Halos which assembled more slowly develop cooling cores at lower mass than those that assemble more rapidly, in agreement with Yoshida et al. (2003). We do, however, observe significant evolution in the accretion rates of Population III protostars with redshift, with objects that form later having higher maximum accretion rates, with a variation of two orders of magnitude (10^-4 - 10^-2 Msolar/year). This can be explained by considering the evolving virial properties of the halos with redshift and the physics of molecular hydrogen formation at low densities. Our result implies that the mass distribution of Population III stars inferred from their accretion rates may be broader than previously thought, and may evolve with redshift. Finally, we observe that our collapsing protostellar cloud cores do not fragment, consistent with previous results, which suggests that Population III stars which form in halos of mass 10^5 - 10^6 Msun always form in isolation.Comment: Accepted by The Astrophysical Journal. Some minor changes. 65 pages, 3 tables, 21 figures (3 color). To appear in January 1, 2007 issu

The Effects of Electromyographic Biofeedback on Test Anxiety and Performance

The applicability of electromyographic biofeedback toward alleviating test taking anxiety was examined along with the effects of relaxation training on general anxiety, locus of control, test performance, and muscle tension during a test. The Achievement Anxiety Test (AAT) was administered to 271 freshman psychology students. Students whose scores indicated high levels of test anxiety were invited to participate in the study. Twenty-seven volunteers were randomly assigned to three groups. Biofeedback (B) subjects received verbal instructions and muscle tension (EMG) biofeedback. Instruction-control (IC) subjects received verbal relaxation instructions alone. A second control group (C) received no treatment. B and IC subjects received eight half-hour relaxation sessions spread over four weeks. Forehead EMG was monitored during each session. Several self-report measures were administered to all subjects before and after training. They included the AAT, the State-Trait Anxiety Inventory (STAI), and the Rotter Locus of Control (I-E) Scale. Additionally, forehead muscle tension data were collected on all subjects while they completed an easy and a hard form of the Raven Progressive Matrices test (presented with ego-involving instructions). Equivalent forms of the tests were used pre and post, and the forms were counterbalanced across subjects. Analysis of the EMG data collected during relaxation training indicated that B and IC subjects significantly reduced forehead muscle tension but did not differ from each other. EMG biofeedback appears to add little to the effectiveness of brief relaxation instructions and practice. Analysis of anxiety measures indicated that B and IC subjects changed significantly pre to post while C subjects changed very little. No between -group differences were found on any of the measures. On the I-E scale, only IC subjects showed a significant shift toward being more internal, reflecting an increased belief in personal control. The effect of relaxation training on test performance and muscle tension during testing was evaluated with an analysis of variance. Within-group EMG and performance changes were nonsignificant, suggesting there was no generalization of training effects. In addition, EMGs did not differ between Easy and Hard tests, although performance scores indicated there were real differences in test difficulty. The effects of relaxation training in this study are clearly limited to the reduction of resting forehead muscle tension and self-report anxiety. No training effects were found on test performance or EMG during testing. These results are due either to the methodological limitations of a laboratory testing situation, or that forehead EMG is not as good a measure of anxiety as other researchers have suggested

Reliability and validity of the observational form of the Child Behavior Checklist

A review of observational coding systems indicated the need for an instrument which is easy to learn and implement in applied settings, and which can be applied to diverse populations of disturbed children. A two phase study was conducted to evaluate the reliability and validity of observational ratings derived from the Direct Observation Form (DOF) of the Child Behavior Checklist. The DOF contains 96 behavior problem items (drawn from the Child Behavior Checklist) and a measure of on-task behavior. Observational periods are 10 minutes in length and samples of behavior are taken on six occasions. Observers write a narrative description of behavior noting the occurrence and frequency of any behavior problems. On-task behavior is rated every minute for five seconds. After each observational period the 96 checklist items are rated on a 0-1-2-3 scale. Scores are derived by summing the ratings of on-task and problem behavior across the six sessions. In Phase one of the study, the behaviors of 25 referred boys (aged 6-11) were rated by two observers in classroom settings. Checklist data from parents and teachers were also collected. Observer agreement on ratings of on-task and problem behavior was very good. Concurrent validity and the discriminant validity of individual behavioral items among observers, parents, and teachers was adequate considering the differences in setting and amounts of child contact. The temporal stability of ratings was poor, reflecting the high variability of behavior among referred children. In Phase two, 15 matched pairs of teacher-referred and nonreferred boys (aged 6-11) were observed in regular classroom settings. Checklist data were also collected from teachers. Significant differences were found between the groups for problem and on-task behavior; which suggests predictive validity. Relations among teacher and observer ratings were indicative of concurrent validity. Based on a crude classification strategy, screening efficiency was good for problem behavior (misclassification rate of 16.5 percent) and poor for on-task behavior (misclassification rate of 43 percent). Overall it was concluded that the DOF represents a reliable and valid indicator of children's problem behavior. Ways of improving screening efficiency are discussed. In addition, it was suggested that the development of syndromes and construction of norms would increase the usefulness of observational ratings for clinicians and researchers