167 research outputs found
Functional genomic approaches and genome-wide transcript profiles for the investigation of plant responses towards powdery mildew infection
CRISPR/Cas9 advances engineering of microbial cell factories
One of the key drivers for successful metabolic engineering in microbes is the efficacy by which genomes can be edited. As such there are many methods to choose from when aiming to modify genomes, especially those of model organisms like yeast and bacteria. In recent years, clustered regularly interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become the method of choice for precision genome engineering in many organisms due to their orthogonality, versatility and efficacy. Here we review the strategies adopted for implementation of RNA-guided CRISPR/Cas9 genome editing with special emphasis on their application for metabolic engineering of yeast and bacteria. Also, examples of how nuclease-deficient Cas9 has been applied for RNA-guided transcriptional regulation of target genes will be reviewed, as well as tools available for computer-aided design of guide-RNAs will be highlighted. Finally, this review will provide a perspective on the immediate challenges and opportunities foreseen by the use of CRISPR/Cas9 genome engineering and regulation in the context of metabolic engineering
Effect of foot orthotic on bone alignment:Foot and lower limb 3D reconstruction from EOS® biplanar radiographic imaging
PILOT STUDY OF CARTILAGE CREEP AND RECOVERY DETERMINED WITH NONINVASIVE MAGNETIC RESONANCE IMAGING AND CLASSICAL MECHANICAL TESTING
Development and validation of subject-specific moving-axis knee model using MRI and EOS imaging during quasi-static movements
Development and evaluation of a subject-specific lower limb model with an eleven-degrees-of-freedom natural knee model using magnetic resonance and biplanar x-ray imaging during a quasi-static lunge
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