Jensen, Michael Krogh

Keasling, Jay

Skjødt, Mette Louise

Snoek, Tim

The present invention relates to the use of transcriptional activators from prokaryotic organisms for use in eukaryotic cells, such as yeast as sensors of intracellular and extracellular accumulation of a ligand or metabolite specifically activating this transcriptional activator in a eukaryot, such as yeast cell, such as a cell engineered to produce this ligand. The transcriptional activator controls a promoter upstream of one or more gene, which may include e.g. a reporter gene that may be a fluorescence marker, such as luciferase, green fluorescent protein or a gnee encoding antibiotic resistance

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Apr 20, 2018Use of prokaryotic transcriptional activators as metabolite biosensors in eukaryoticcellsJensen, Michael Krogh; Keasling, Jay; Skjødt, Mette Louise; Snoek, TimPublication date:2018Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Jensen, M. K., Keasling, J., Skjødt, M. L., & Snoek, T. (2018). IPC No. C12N15/67; C12N15/81. Use ofprokaryotic transcriptional activators as metabolite biosensors in eukaryotic cells (Patent No. WO2017EP66943.)

Use of prokaryotic transcriptional activators as metabolite biosensors in eukaryotic cells

http://orbit.dtu.dk/ws/files/146141150/WO2018007526A1.pdf

Use of prokaryotic transcriptional activators as metabolite biosensors in eukaryotic cells

Abstract

Similar works

Full text

Available Versions

Online Research Database In Technology