Cross-Platform Comparison of Untargeted and Targeted Lipidomics Approaches on Aging Mouse Plasma.

Lipidomics - the global assessment of lipids - can be performed using a variety of mass spectrometry (MS)-based approaches. However, choosing the optimal approach in terms of lipid coverage, robustness and throughput can be a challenging task. Here, we compare a novel targeted quantitative lipidomics platform known as the Lipidyzer to a conventional untargeted liquid chromatography (LC)-MS approach. We find that both platforms are efficient in profiling more than 300 lipids across 11 lipid classes in mouse plasma with precision and accuracy below 20% for most lipids. While the untargeted and targeted platforms detect similar numbers of lipids, the former identifies a broader range of lipid classes and can unambiguously identify all three fatty acids in triacylglycerols (TAG). Quantitative measurements from both approaches exhibit a median correlation coefficient (r) of 0.99 using a dilution series of deuterated internal standards and 0.71 using endogenous plasma lipids in the context of aging. Application of both platforms to plasma from aging mouse reveals similar changes in total lipid levels across all major lipid classes and in specific lipid species. Interestingly, TAG is the lipid class that exhibits the most changes with age, suggesting that TAG metabolism is particularly sensitive to the aging process in mice. Collectively, our data show that the Lipidyzer platform provides comprehensive profiling of the most prevalent lipids in plasma in a simple and automated manner

Hylaeogena cincta Waterhouse 1889

Hylaeogena cincta (Waterhouse, 1889) Pachyschelus cinctus Waterhouse, 1889: 149. Hylaeogena cincta: Hespenheide, 1974: 106. Hylaeogena circumciliata Cobos, 1967: 222, syn. nov. In comparing the type specimen of H. cincta from BMNH with type images of H. circumciliata from MNCN, no significant differences could be found, hence these two species are considered conspecific. This synonymy is also underscored by a high probability of distribution of the species throughout Central America. Specimens examined: BMNH: 1 ex.: V. de Chiriqui, 3-4000 ft. Champion | Pachyschelus cinctus Type Waterh.; 1 ex.: V. de Chiriqui, 2-3000 ft. Champion.; 1 ex: Rio Maria Linda, 500 ft. Champion. MNCN: 1 ex.: Mexico [h] | female [p, symbol] | Holotypus / A. Cobos [p, red label] | Ex Coleccion/ Dr. A. Cobos [p, yellow label] | Hylaeogena / circumciliata / nov. sp./ Holotipo [h]/ A. Cobos det. 195 [p] 9 [h] | MNCN / Cat. Tipos N° [p]/ 2117 [h] [red label] | MNCN _Ent/ N° Cat. 56860 [p].Published as part of Hornburg, Michael, 2012, Nomenclatural changes in Neotropical Tracheini with the description of Hylaeogena aeneonitens sp. nov. (Coleoptera: Buprestidae) from the Orinoco basin in Venezuela, pp. 53-61 in Zootaxa 3242 on page 55, DOI: 10.5281/zenodo.28049

Hylaeogena circularis Kerremans 1899

Hylaeogena circularis (Kerremans, 1899) Pachyschelus circularis Kerremans, 1899: 354. Hylaeogena circularis: Hespenheide, 1979: 112. Hylaeogena bruchi (Kerremans, 1903) syn. nov. Pachyschelus bruchi Kerremans, 1903: 318. Hylaeogena bruchi: Obenberger, 1925: 143. Hylaeogena scutellaris Obenberger, 1925: 146 syn. nov. In comparing the holotype of H. circularis, the syntypes of H. bruchi from BMNH and the syntypes of H. scutellaris from NMPC, no significant difference was found and hence all examined specimens are considered conspecific. Specimens examined: BMNH: 1 ex.: Holo-/ type [p, round label, red margin] | Jatahy/ Pujol. [h] | circularis / Kerr./ Type [h]. 1 ex.: Syn-/ type [round label, blue margin, p] | Rep. Brasil [p]/ 5.II. [h] 190 [p] 1 [h] | Bruchi / Kerrem./ Type [h] | P. Bruchi / Kerrem. Bresil [h] | Kerremans/ 1903 – 59 [p]; 1 ex.: Syn-/ type [round label, blue margin, p] | Bresil / Bruchi [h] | Bruchi / Kerrem./ Type [h] | Kerremans/ 1903 – 59 [p]. NMPC: 2 ex.: Rep. Brasil / 17. III. 1901 / C.Bruch [p, h] | TYPUS [p, red label] | Hylaeogena / scutellaris m. Type [h]/ Det Dr. Obenberger [p].Published as part of Hornburg, Michael, 2012, Nomenclatural changes in Neotropical Tracheini with the description of Hylaeogena aeneonitens sp. nov. (Coleoptera: Buprestidae) from the Orinoco basin in Venezuela, pp. 53-61 in Zootaxa 3242 on page 56, DOI: 10.5281/zenodo.28049

Hylaeogena circumdata Kerremans 1897, comb. nov.

Hylaeogena circumdata (Kerremans, 1897) comb. nov. Pachyschelus circumdatus Kerremans, 1897: 126. Hylaeogena achardi Obenberger, 1925: 145 syn. nov. From all morphological aspects from the generic diagnosis, Pachyschelus circumdatus belongs to Hylaeogena. During the comparison of the holotype of H. circumdata from BMNH and the syntypes of H. achardi from NMPC, no significant differences were found, and these two species are considered to be conspecific. Specimens examined: BMNH: 1 ex.: Holo-/ type [round label, red margin, p] | Tijuca (Rio)/ Bresil / E.Gounelle 12.1884 [p] | circumdatus / Kerr./ Type [h] | P. circumdatus / Kerr. Mem. Belg. 1897.126 Rio [h] | Kerremans/ 1903. 59 [p]; 1 ex.: 18309 [h] | Fry/ Rio Jan. [p] | Fry Coll./ 1905 – 100 [p] NMPC: 1 ex.: Sao Paulo/ Brs. Mráz [p] | TYPUS [p, red label] | Achardi m. 3 Type [h]/ Det Dr. Obenberger; 1 ex.: Sao Paulo/ Brs. Mráz [p] | TYPUS [p, red label] | Achardi m. Ƥ Type [h]/ Det Dr. Obenberger [p].Published as part of Hornburg, Michael, 2012, Nomenclatural changes in Neotropical Tracheini with the description of Hylaeogena aeneonitens sp. nov. (Coleoptera: Buprestidae) from the Orinoco basin in Venezuela, pp. 53-61 in Zootaxa 3242 on page 56, DOI: 10.5281/zenodo.28049

Hylaeogena capitata Kerremans 1903, comb. nov.

Hylaeogena capitata (Kerremans, 1903) comb. nov. Pachyschelus capitatus Kerremans, 1903: 318. This species belongs to Hylaeogena due to all morphological aspects in the original genus diagnosis by Obenberger (1923). Species examined: BMNH: 1 ex.: Holo-/ type [round label, red margin, p] | Jatahy/ Donckien [h] | P. capitatus / Kerremans Goyaz [h] | Not Pachyschelus,/ Hylaeogena [h]/ B. Levey det. 1972 [p]. MNHN: 1 ex.: Jahaty/ P. de Goyaz/ Brésil [p] | capitatus / Kerr. Type [h]; 3 ex.: Jahaty/ P. de Goyaz/ Brésil [p] | capitatus / Kerr. [h]/ Paratype [p].Published as part of Hornburg, Michael, 2012, Nomenclatural changes in Neotropical Tracheini with the description of Hylaeogena aeneonitens sp. nov. (Coleoptera: Buprestidae) from the Orinoco basin in Venezuela, pp. 53-61 in Zootaxa 3242 on page 55, DOI: 10.5281/zenodo.28049

Hylaeogena carbo Kirsch 1866

Hylaeogena carbo (Kirsch, 1866) Brachys carbo Kirsch, 1866: 179. Hylaeogena carbo: Obenberger, 1925: 24, 131, 139. The holotype should be in the collection of the SMTD, but it could neither be found by me nor by the collection manager Mr. O. Jäger and is assumed to be lost.Published as part of Hornburg, Michael, 2012, Nomenclatural changes in Neotropical Tracheini with the description of Hylaeogena aeneonitens sp. nov. (Coleoptera: Buprestidae) from the Orinoco basin in Venezuela, pp. 53-61 in Zootaxa 3242 on page 57, DOI: 10.5281/zenodo.28049

Pachyschelus lunifer Waterhouse 1889

Pachyschelus lunifer Waterhouse, 1889 original combination reinstated Pachyschelus lunifer Waterhouse, 1889: 148. Hylaeogena lunifer: Hespenheide, 1974: 106. This species was inadvertently transferred to Hylaeogena (Hespenheide 1974). After examination, based on the genus diagnosis, it belongs to Pachyschelus and is herewith returned to the original combination. Specimen examined: BMNH: holotype: Guatemala, Volcan de Atitlan 3000 feet (Champion).Published as part of Hornburg, Michael, 2012, Nomenclatural changes in Neotropical Tracheini with the description of Hylaeogena aeneonitens sp. nov. (Coleoptera: Buprestidae) from the Orinoco basin in Venezuela, pp. 53-61 in Zootaxa 3242 on page 57, DOI: 10.5281/zenodo.28049

Hylaeogena (Hylaeogena) aeneonitens Hornburg, 2012, sp. nov.

Hylaeogena (Hylaeogena) aeneonitens sp. nov. (Figs. 1, 2, 4) Type specimens. Holotype (3 Fig. 1, 2): Venezuela, Edo. Anzoátegui,/ Soledad, nr. Ciudad Bolivar,/ 08°09’ 18 ’’N, 63 ° 33 ’ 28 ’’W, 30 m / 6.VIII. 2001, leg. M. Hornburg [p, green label]. Paratypes: 20 ex. (113, 9 ƤƤ): same data as holotype; 8 ex. (2 3, 6 ƤƤ): Venezuela (Anzoátegui):/ Soledad, 30 m,/ nr. Ciudad Bolivar/ 08°09’N, 63 ° 33 ’W / 6.VIII. 2001 / S. Gottwald leg. [p, green label]; 1 ex.: Venezuela-GU [Guarico]/ Calabozo [p]/ 14 - 4 - [h] 196 [p] 5 [h]/ J. & B. Bechyne [p] | Venezuela-Inst./ Zool. Agricola/ Fac. Agronomia/ Univ. Central [p, green label] General diagnosis. length: 2.3–2.5 mm, width: 1.5–1.6 mm, dorsal surface dark bronze, shiny. Body egg-shaped, eyes flat. Females with dentate anal ventrite. Description of Holotype. Measurements: total length: 2.40 mm; width: 1.52 mm. Head bronze; eyes flat, nearly concave; inner margins of eyes parallel; widely spaced punctation on very fine, shiny microsculptured frons; glabrous. Antennae 11 -jointed; antennomeres 1, 2 enlarged; broadened from the antennomere 7. Pronotum bronze; base three times broader than length, in body axis; lateral margins regularly arcuate, converging anteriorly; anterior margin simply rounded; very fine, shiny microsculptured; without punctation, only some irregularly arranged smooth spots; glabrous. Scutellum like head and pronotum, very fine, shiny, but regularly microsculptured; triangular; two times as broad as long; glabrous. Elytra bronze-coloured; surface smooth, without any microsculpture; broadest in anterior third, longitudinal puncture rows clearly visible; hardly noticeable single setae arise from these pores; between puncture-rows slightly wrinkled; apicolateral half more rugose; humeral bulb and posthumeral depression distinct; lateral margin feebly serrate in apical third. Ventral side blackish; very fine microsculptured, like pronotum; anal ventrite simple, only with transverse apical furrow. Legs with typical strongly flattened tibiae, same colour as ventral surface. Male genitalia claviform (Fig 4). Etymology: The species is named in reference to its bronze, shiny colouring. Distribution: Venezuela (Anzoátegui, Guarico). Differential diagnosis Hylaeogena aeneonitens in general shape, and especially the flattened eyes, is closely related to H. lecerfi Obenberger, 1932 from Panama and H. nana (Kirsch, 1866) from Peru. However, the scutellum of H. nana is nearly equilateral and the general surface more shiny, with a slight greenish tinge on the pronotum, while H. aeneonitens is unicoloured. H. aeneonitens can be distinguished from the blackish H. lecerfi by the colour, the elytral pilosity and the distinct pronounced posthumeral depression. The holotype and paratypes will be deposited in the ZMHB. Paratypes will be deposited in the MIZA, NMPC, BMNH, SGCB and MHCB.Published as part of Hornburg, Michael, 2012, Nomenclatural changes in Neotropical Tracheini with the description of Hylaeogena aeneonitens sp. nov. (Coleoptera: Buprestidae) from the Orinoco basin in Venezuela, pp. 53-61 in Zootaxa 3242 on page 59, DOI: 10.5281/zenodo.28049