Design of RNAi reagents for invertebrate model organisms and human disease vectors

RNAi has become an important tool to silence gene expression in a variety of organisms, in particular when classical genetic methods are missing. However, application of this method in functional studies has raised new challenges in the design of RNAi reagents in order to minimize false positive and false negative results. Since the performance of reagents can be rarely validated on a genome-wide scale, improved computational methods are required that consider experimentally derived design parameters. Here, we describe computational methods for the design of RNAi reagents for invertebrate model organisms and human disease vectors, such as Anopheles. We describe procedures on how to design short and long double-stranded RNAs for single genes, and evaluate their predicted specificity and efficiency. Using a bioinformatics pipeline we also describe how to design a genome-wide RNAi library for Anopheles gambiae

Leadership and capability development and deployment in the New Zealand State Service

This article describes the design during 2013 of a model and implementation principles of a leadership and capability development and deployment (LCDD) model for the state services system. In this process, an initial prototype model was developed to describe a desired future state. It was informed by the best traditions of state services leadership development, together with models used by the world’s best companies, such as Procter & Gamble, for leadership development (Filipkowski and Donlon, 2013). The model was then enhanced based on our research in other jurisdictions, including Australia, Singapore and the United Kingdom, and co-creation with stakeholders. This is further discussed in the article. Dr Mike Pratt is Professor of Leadership and Sustainability at the University of Waikato, and a professional director and business adviser. Dr Murray Horn is a former Secretary to the Treasury of New Zealand and bank chief executive, and a professional director and business adviser

Ecology of fishes in Upper Newport Bay, California: seasonal dynamics and community structure

A total of 366 bimonthly (January 1978-January 1979) samples taken with six types of gear (otter trawl, gill net, bag seine, small seine, drop net, square enclosure - all with replication except the gill net) at four stations in upper Newport Bay, California yielded 51,816 fishes belonging to 46 species and weighing over 353 kg. Atherinops affinis (topsmelt) was the most abundant species accounting for 76% of total individuals. Seven species, all of low trophic levels, made up over 97% of the total catch. Mugil cephalus (striped mullet) ranked first in biomass (= 36% of the total) with six species accounting for more than 80% of the total biomass. The largest number of individuals (71%) was collected with the bag seine, the greatest number of species (35) was captured with the otter trawl and the largest percentage of the biomass (56%)was obtained with the gill net. Species richness, number of individuals and biomass were lowest in January (1978 or 1979) or March and highest in July (numbers, biomass) or September (species). Bimonthly diversity (H') values ranged from 0.48 to 2.17 (overall value 1.05) and tended to be inversely related to abundance levels. Species richness was greatest at Station 4 (the lowermost station) and least at Station 1 (the uppermost station). Numbers of individuals and biomass peaked at Station 2 and reached lowest levels at Station 1. Length-frequency analysis of six of the most abundant species indicated utilization of the upper bay by two or more stages in the life history of these species. More than 92,000 eggs belonging to seven taxa and an unknown category and 426 larvae from 20 taxa were collected with a 0.5 m net mounted on an epibenthic sled during the same bimonthly periods and at the same stations as the juvenile/adult samples. Most of the eggs were collected at Station 2 in May with the numbers overwhelmingly dominated by those of Anchoa compressa (deepbody anchovy) (99.7% of total numbers). The most abundant larva was that of Clevelandia ios (arrow goby). Nearly 60% of the total larval catch was made up of members of the family Gobiidae. Larval taxa and individuals were fewest in January (1978). The number of taxa was highest in March, September and January (1979) whereas larval numbers peaked in May. The number of taxa and of individual larvae varied only slightly among the four stations. Asymptotic species accumulation curves indicated adequate sampling of juvenile/adult fishes. Cluster analysis produced eight species groups of resident and periodic species that variously utilize the three main habitats (channel, inshore, pannes) in the upper bay. Species richness and abundance were positively correlated with both temperature and salinity. Temperature, salinity and depth of capture were frequently correlated with individual species abundances and were used in combination to partially explain the spatial utilization of species and species groups. The upper bay fish community is important and worthy of preservation for at least three reasons: 1) it contains species assemblages not duplicated in any other coastal environment; 2) it contains life history stages of a variety of coastal fish species; and 3) it contains large populations of small, low-trophic level species and juveniles of other species which serve as forage for larger, predatory species that are frequently of economic importance. Members of the fish community respond noticeably to altered environmental conditions such as the heavy rainfall (and accompanying low salinity and high turbidity) that occurred during the early months of 1978. The short and long term, as yet often unpredictable, fluctuations in the populations emphasize the need for periodic monitoring and for the development of a mathematical model of the fish community if it is to be thoroughly understood and properly managed. (102pp.

River Run Off Measurement With SAR Along Track Interferometry

The paper summarizes the need for global space borne river run-off measurements. It reports about an airborne SAR experiment aimed to measure the surface velocity of the river Isar in Bavaria / Germany. The results from two different SAR techniques, including Along Track Interferometry (ATI) show good correspondence. Finally suggestions for further studies are given