Characterization of a Double Mesospheric Bore Over Europe

Observations of a pair of mesospheric bore disturbances that propagated through the nighttime mesosphere over Europe are presented. The observations were made at the Padua Observatory, Asiago (45.9\ub0N, 11.5\ub0E), by the Boston University all-sky imager on 11 March 2013. The bores appeared over the northwest horizon, approximately 30 min apart, and propagated toward the southeast. Using additional satellite and radar data, we present evidence indicating the bores originated in the mesosphere from a single, larger-scale mesospheric disturbance propagating through the mesopause region. Furthermore, the large-scale mesospheric disturbance appeared to be associated with an intense weather disturbance that moved southeastward over the United Kingdom and western Europe during 10 and 11 March

Airglow imaging observations in Germany and Norway

第2回極域科学シンポジウム/第35回極域宙空圏シンポジウム 11月15日（火） 国立極地研究所 2階大会議室前フロ

Temporal variability of tidal and gravity waves during a record long 10-day continuous lidar sounding

Gravity waves (GWs) as well as solar tides are a key driving mechanism for the circulation in the Earth's atmosphere. The propagation of gravity waves is strongly affected by tidal waves as they modulate the mean background wind field and vice versa, which is not yet fully understood and not adequately implemented in many circulation models. The daylight-capable Rayleigh–Mie–Raman (RMR) lidar at Kühlungsborn (54°N, 12°E) typically provides temperature data to investigate both wave phenomena during one full day or several consecutive days in the middle atmosphere between 30 and 75km altitude. Outstanding weather conditions in May 2016 allowed for an unprecedented 10-day continuous lidar measurement, which shows a large variability of gravity waves and tides on timescales of days. Using a one-dimensional spectral filtering technique, gravity and tidal waves are separated according to their specific periods or vertical wavelengths, and their temporal evolution is studied. During the measurement period a strong 24h wave occurs only between 40 and 60km and vanishes after a few days. The disappearance is related to an enhancement of gravity waves with periods of 4–8h. Wind data provided by ECMWF are used to analyze the meteorological situation at our site. The local wind structure changes during the observation period, which leads to different propagation conditions for gravity waves in the last days of the measurement period and therefore a strong GW activity. The analysis indicates a further change in wave–wave interaction resulting in a minimum of the 24h tide. The observed variability of tides and gravity waves on timescales of a few days clearly demonstrates the importance of continuous measurements with high temporal and spatial resolution to detect interaction phenomena, which can help to improve parametrization schemes of GWs in general circulation models

Lidar Soundings Between 30 and 100 km Altitude During Day and Night for Observation of Temperatures, Gravity Waves and Tides

Ground-based temperature measurements by lidar are an important tool for the understanding of long-term temperature changes as well as the propagation of gravity waves and tides. Though, mesospheric soundings are often limited to nighttime conditions due to the low signal-tonoise ratio during the day. We developed a daylight-capable RMR lidar for temperature soundings in the middle atmosphere. The influences of the narrowband detector on the calculated hydrostatic temperatures as well as their correction are described. The RMR lidar is complemented by a co-located resonance lidar. We present an example for tidal analyses and short-term variability of tidal amplitude

Local stratopause temperature variabilities and their embedding in the global context

The stratopause is by definition the transition between the stratosphere and mesosphere. During winter the circulation at mid-latitudes and high latitudes in the stratosphere is mainly driven by quasi-stationary planetary waves (PWs), while the circulation in the mesosphere is mainly driven by gravity waves (GWs). The question arises of whether PWs or GWs dominate the variability of the stratopause. The most famous and dramatic variability of the middle atmosphere is a sudden stratospheric warming (SSW) generated by PWs interacting with the polar vortex. A similar phenomenon but smaller in magnitude and more regional is stratopause temperature enhancements (STEs) initially observed by local measurements and generated by breaking PWs. Thus it seems that PWs dominate the variability of the stratopause. In this study we want to quantify to which extent quasi-stationary PWs contribute to the stratopause variability. To do that we combine local lidar observations at Kühlungsborn (54∘ N, 11∘ E) and Andenes (69∘ N, 16∘ E) with global MERRA-2 reanalysis data bringing the local variability of the stratopause into the global context. Therefore we compare the temperature time series at Kühlungsborn and Andenes at 2 hPa, the altitude where STEs maximize, with characteristics (amplitude and phase) of PWs with wave numbers 1, 2 and 3. We found that for Kühlungsborn and Andenes 98 % of the local day-to-day variability of the stratopause can be explained by the variability of PWs with wave number 1, 2 and 3. Thus, the winter stratopause day-to-day variability is highly dominated by the variability of PWs

High-throughput, quantitative analyses of genetic interactions in E. coli.

Large-scale genetic interaction studies provide the basis for defining gene function and pathway architecture. Recent advances in the ability to generate double mutants en masse in Saccharomyces cerevisiae have dramatically accelerated the acquisition of genetic interaction information and the biological inferences that follow. Here we describe a method based on F factor-driven conjugation, which allows for high-throughput generation of double mutants in Escherichia coli. This method, termed genetic interaction analysis technology for E. coli (GIANT-coli), permits us to systematically generate and array double-mutant cells on solid media in high-density arrays. We show that colony size provides a robust and quantitative output of cellular fitness and that GIANT-coli can recapitulate known synthetic interactions and identify previously unidentified negative (synthetic sickness or lethality) and positive (suppressive or epistatic) relationships. Finally, we describe a complementary strategy for genome-wide suppressor-mutant identification. Together, these methods permit rapid, large-scale genetic interaction studies in E. coli