37 research outputs found

    Egernia stokesii MHC I and II genotypes and alleles 2012-13

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    Egernia stokesii were sampled across three populations in the Flinders Ranges of South Australia during the Austral summer of 2012-13. The data file contains genotypes for 66 lizards for MHC I (35 alleles) and 34 lizards for MHC II (5 alleles)

    Egernia stokesii microsatellite genotypes 2012-13

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    Egernia stokesii were sampled across three populations in the Flinders Ranges, South Australia during the Austral summer of 2012-13. The data file contains microsatellite genotypes (11 loci) for 66 lizards

    Exoneurella eremophila nest collections, Mildura Australia

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    Nest census data for Exoneurella eremophila. Colonies were collected from Mildura, Victoria, Australia during spring: 11-13 October 2013, summer: 21-23 January 2014 and autumn: 12-14 April 2014. These are represented as Trip 2-4 respectively in the data. The nest length (mm), number of adult females, males eggs, larvae and pupae present in the nest on collection are recorded. 'Broodyn' indicates whether the nest had brood (Y) or not (N). 'Socialyn' indicates whether the nest was social (Y) or not (N). Sex ratio was calculated as: sex.ratio = male.pupae/tot.pupae. Per capita brood production was calculated as: percap = tot.brood/female

    MHCsequences

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    Nucleotide sequences pertaining to 31 MHC I alleles and 5 MHC II alleles used to derive measures in E. stokesii mate choice tests

    MHCgenotypes

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    Contains MHC I genotypes for 47 adult E. stokesii and MHC II genotypes for 46 adult E. stokesii, utilised in mate choice tests

    MICSATgenotypes

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    Microsatellite genotype data (six loci) for adult E. stokesii, utilised in mate choice tests

    Novel RNA viruses within plant parasitic cyst nematodes

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    <div><p>The study of invertebrate–and particularly nematode–viruses is emerging with the advancement of transcriptome sequencing. Five single-stranded RNA viruses have now been confirmed within the economically important soybean cyst nematode (SCN; <i>Heterodera glycines</i>). From previous research, we know these viruses to be widespread in greenhouse and field populations of SCN. Several of the SCN viruses were also confirmed within clover (<i>H</i>. <i>trifolii</i>) and beet (<i>H</i>. <i>schachtii</i>) cyst nematodes. In the presented study, we sequenced the transcriptomes of several inbred SCN populations and identified two previously undiscovered viral-like genomes. Both of these proposed viruses are negative-sense RNA viruses and have been named SCN nyami-like virus (NLV) and SCN bunya-like virus (BLV). Finally, we analyzed publicly available transcriptome data of two potato cyst nematode (PCN) species, <i>Globodera pallida</i> and <i>G</i>. <i>rostochiensis</i>. From these data, a third potential virus was discovered and called PCN picorna-like virus (PLV). PCN PLV is a positive-sense RNA virus, and to the best of our knowledge, is the first virus described within PCN. The presence of these novel viruses was confirmed via qRT-PCR, endpoint PCR, and Sanger sequencing with the exception of PCN PLV due to quarantine restrictions on the nematode host. While much work needs to be done to understand the biological and evolutionary significance of these viruses, they offer insight into nematode ecology and the possibility of novel nematode management strategies.</p></div
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