final_dataset_and_input_files

This archive contains several output files from Stacks for the final dataset of 723 SNPs across 23 samples of Labeobarbus natalensis. We also include input files for each of the downstream analysis programs used in this study for the final dataset. The formats given include .arp, .genepop, .gtx, .structure, .vcf and .geste. For a more detailed breakdown of each file, please refer to the ReadMe

raw_RAD_data_replicated_samples

This archive contains the raw RAD sequencing data files in paired-end FastQ format for the two replicated Labeobarbus natalensis samples used in the study, BLU004 (BLA004) and KNU017 (KNB017). Sequence data was generated by an Illumina HiSeq 2000. This data has not undergone initial filtering by the sequencing company. FastQ files are in the standard format, with all barcodes and adapters trimmed (apart from any pollution due to overlap). The two samples are presented differently - for more information please refer to the ReadMe and the publication

2‑(Diethyl)aminoethyl Methacrylate as a CO₂‑Switchable Comonomer for the Preparation of Readily Coagulated and Redispersed Polymer Latexes

CO₂ stimuli-responsive polystyrene latexes having a solids content of 27% were prepared in a surfactant-free emulsion polymerization (SFEP) under a CO₂ atmosphere, employing only commercially available chemical compounds: styrene, the initiator VA-061, and 0.54 mol % of the CO₂-switchable comonomer DEAEMA. The resulting polymer particles are 230–300 nm in diameter and are monodisperse (PDI ≤ 0.054), as confirmed by DLS, TEM, and SEM. Although they are stable under a CO₂ atmosphere, the latexes can be easily destabilized by the bubbling of air through the sample at 40 °C, allowing for recovery of the particles by filtration. Recovered polymer particles can be dried to powder and readily redispersed in carbonated water, yielding latexes with very similar zeta-potential and particle size as the original latexes. In addition, the bicarbonate salt of poly­(DEAEMA) formed during the polymerization has been found to act as a CO₂-switchable flocculant, thus, facilitating the coagulation of the latex without altering the properties of the latex after redispersion

Ldayi_39-40

Alignment for Litoria dayi sequences less than 200 base pairs long at locus MVZ 39/40 X. laevis MGC82783 protein (Intron2)

Ldayi_CRYBA

Alignment for Litoria dayi sequences less than 200 base pairs long at locus Cryb1LS/Cryb2LS Beta-crystallin

Lnannotis_19-20

Alignment of Litoria nannotis sequences less than 200 base pairs long at locus MVZ19/MVZ20 Myosin Light Chain (Intron 6)

Lrheocola_39-40

Alignment of Litoria rheocola sequences less than 200 base pairs long at locus MVZ39/MVZ40 X. laevis MGC82783 protein (Intron2)

Lserrata_29-30

Alignment of Litoria serrata sequences less than 200 base pairs long at locus MVZ29/MVZ30 Y Box Binding (Intron 1)

Lnannotis_29

Alignment of Litoria nannotis sequences less than 200 base pairs long at locus MVZ29/MVZ30 Y Box Binding (Intron 1)

Lserrata_39-40

Alignment of Litoria serrata sequences less than 200 base pairs long at locus MVZ39/MVZ40 X. laevis MGC82783 protein (Intron2)