62 research outputs found
The State and the Sacred: Memory, Theology, and Identity in Kyrgyzstan
Since becoming independent in 1991, Kyrgyzstan has endured two revolutions, ethnic violence, and economic decay. Against this tumultuous background have been ongoing attempts to construct a viable Kyrgyz nationalism and the “resurgence” of religion in the public sphere. In many respects, however, these phenomena have been conceptualized as being largely independent of one another. Similarly, religion – and Islam in particular – has sometimes been depicted as an ideological “alternative” to a congenitally weak and fractured Kyrgyz national identity, and as an autonomous force to be confronted, tamed, and instrumentalized by the state. This dissertation seeks to reassess the seemingly fraught nature of the relationship between religion, politics, and identity. Drawing on fieldwork conducted in Kyrgyzstan in 2014, it argues that religious and political geographies should not be viewed as fundamentally alienated from one another. Rather, the territorial logic of the nation-state inevitably exerts a powerful influence on the religious imaginary, while religion in turn constitutes a crucial site for the formation of national identity and the legitimation of state power. This dynamic points to the enduring centrality of the nation-state during an era of continuing globalization, as well as the need for further attention devoted to understanding and acknowledging the critical importance of religious discourses in the constructing political identities and geographies
The tuberculosis necrotizing toxin kills macrophages by hydrolyzing NAD.
Mycobacterium tuberculosis (Mtb) induces necrosis of infected cells to evade immune responses. Recently, we found that Mtb uses the protein CpnT to kill human macrophages by secreting its C-terminal domain, named tuberculosis necrotizing toxin (TNT), which induces necrosis by an unknown mechanism. Here we show that TNT gains access to the cytosol of Mtb-infected macrophages, where it hydrolyzes the essential coenzyme NAD(+). Expression or injection of a noncatalytic TNT mutant showed no cytotoxicity in macrophages or in zebrafish zygotes, respectively, thus demonstrating that the NAD(+) glycohydrolase activity is required for TNT-induced cell death. To prevent self-poisoning, Mtb produces an immunity factor for TNT (IFT) that binds TNT and inhibits its activity. The crystal structure of the TNT-IFT complex revealed a new NAD(+) glycohydrolase fold of TNT, the founding member of a toxin family widespread in pathogenic microorganisms
Nanomolar oxytocin synergizes with weak electrical afferent stimulation to activate the locomotor CPG of the rat spinal cord in vitro.
Synergizing the effect of afferent fibre stimulation with pharmacological interventions is a desirable goal to trigger spinal locomotor activity, especially after injury. Thus, to better understand the mechanisms to optimize this process, we studied the role of the neuropeptide oxytocin (previously shown to stimulate locomotor networks) on network and motoneuron properties using the isolated neonatal rat spinal cord. On motoneurons oxytocin (1 nM-1 \u3bcM) generated sporadic bursts with superimposed firing and dose-dependent depolarization. No desensitization was observed despite repeated applications. Tetrodotoxin completely blocked the effects of oxytocin, demonstrating the network origin of the responses. Recording motoneuron pool activity from lumbar ventral roots showed oxytocin mediated depolarization with synchronous bursts, and depression of reflex responses in a stimulus and peptide-concentration dependent fashion. Disinhibited bursting caused by strychnine and bicuculline was accelerated by oxytocin whose action was blocked by the oxytocin antagonist atosiban. Fictive locomotion appeared when subthreshold concentrations of NMDA plus 5HT were coapplied with oxytocin, an effect prevented after 24 h incubation with the inhibitor of 5HT synthesis, PCPA. When fictive locomotion was fully manifested, oxytocin did not change periodicity, although cycle amplitude became smaller. A novel protocol of electrical stimulation based on noisy waveforms and applied to one dorsal root evoked stereotypic fictive locomotion. Whenever the stimulus intensity was subthreshold, low doses of oxytocin triggered fictive locomotion although oxytocin per se did not affect primary afferent depolarization evoked by dorsal root pulses. Among the several functional targets for the action of oxytocin at lumbar spinal cord level, the present results highlight how small concentrations of this peptide could bring spinal networks to threshold for fictive locomotion in combination with other protocols, and delineate the use of oxytocin to strengthen the efficiency of electrical stimulation to activate locomotor circuits
Reducing cost in a teaching hospital by requiring justification for the use of vecuronium bromide
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