Phantoms of Old Forms: The Gothic Mode in the Dramatic Verse of Tennyson and Browning

My dissertation, “Phantoms of Old Forms: The Gothic Mode in the Dramatic Verse of Tennyson and Browning” situates Alfred Lord Tennyson and Robert Browning within a lineage of writers who experimented with the Gothic mode in dramatic and verse forms. This study is, in the first instance, an experiment in applying a specific strain of Gothic critical theory, one that addresses issues of gender, to canonical literary materials not ordinarily approached in that light. Definitions of the Gothic are notoriously elusive, and this project is not an assertion that the Gothic is always, in all of its manifestations, about gender. Rather, my project draws on a substantial critical history of Gothic literature that identifies gender dynamics within familial relationships as one of many themes within a much broader constellation of Gothic signifiers, though, I would argue, a prominent and important one. In particular this project seeks to illustrate how those critical strategies utilized with Gothic fiction can be adapted and extended to the interpretation of poetry. In fact, the shape and focus of this topic were formed out of a need to redress Gothic scholarship\u27s conspicuous, almost exclusive, emphasis on the genre of novelistic fiction with its roots in prose Romance. While new work has appeared on Gothic tales, Gothic serial writing, and Gothic drama, poetry remains the neglected genre in the critical rehistoricizing of that literary mode. With this in mind, the project proposes as a test case the poetry of Tennyson and Browning, chiefly major dramatic works that were rather unfavorably received in their day and are still somewhat problematic today. In doing so, it proposes that a “Gothic” critical framework draws out implicit features of such texts that represent a critique of patriarchal stabilities in Victorian social ideology. In general terms, Tennyson and Browning, though otherwise more different than alike, shared at least four common points of interest, all of which fell under the purview of early Gothic fiction. Both poets are curious about abnormal emotional and psychological states, though Tennyson is more often associated with the former and Browning more often with the latter. Both poets investigate the troubled relationship between individual identity and larger social structures. Both also relate this trouble to dissonance between and among genders, and lastly, both poets experiment with poetic forms that could give critical expression to this nexus of interrelated concerns. More particularly, this dissertation investigates how these concerns, especially as they pertain to a crisis of masculinity, are expressed through the language and themes of the Gothic with an emphasis on characters who are caught between the dissolving matrices of old power structures and newer, more mobile forms of signification. By utilizing dramatic forms and a Gothic mode to depict these sets of relationships, Tennyson and Browning obliquely critique the evolving social codes through which masculinity was established, recognized, asserted and maintained. The poems that interest me most are the ones that investigate the unraveling of this social “text” within a moment of broader cultural crisis

Biologic activity in a fragment of recombinant human interferon α

To attempt to locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-α2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced two major complementary fragments, HuIFN-α2-(1-110) and HuIFN-α2-(111-153). After reduction with 2-mercaptoethanol and separation of the two major fragments on NaDodSO4/polyacrylamide gel electrophoresis, antiviral activity persisted in the larger, Mr 12,000, fragment consisting of the amino-terminal 110 amino acids

SLIM: an alternative Web interface for MEDLINE/PubMed searches – a preliminary study

BACKGROUND: With the rapid growth of medical information and the pervasiveness of the Internet, online search and retrieval systems have become indispensable tools in medicine. The progress of Web technologies can provide expert searching capabilities to non-expert information seekers. The objective of the project is to create an alternative search interface for MEDLINE/PubMed searches using JavaScript slider bars. SLIM, or Slider Interface for MEDLINE/PubMed searches, was developed with PHP and JavaScript. Interactive slider bars in the search form controlled search parameters such as limits, filters and MeSH terminologies. Connections to PubMed were done using the Entrez Programming Utilities (E-Utilities). Custom scripts were created to mimic the automatic term mapping process of Entrez. Page generation times for both local and remote connections were recorded. RESULTS: Alpha testing by developers showed SLIM to be functionally stable. Page generation times to simulate loading times were recorded the first week of alpha and beta testing. Average page generation times for the index page, previews and searches were 2.94 milliseconds, 0.63 seconds and 3.84 seconds, respectively. Eighteen physicians from the US, Australia and the Philippines participated in the beta testing and provided feedback through an online survey. Most users found the search interface user-friendly and easy to use. Information on MeSH terms and the ability to instantly hide and display abstracts were identified as distinctive features. CONCLUSION: SLIM can be an interactive time-saving tool for online medical literature research that improves user control and capability to instantly refine and refocus search strategies. With continued development and by integrating search limits, methodology filters, MeSH terms and levels of evidence, SLIM may be useful in the practice of evidence-based medicine

Standards for Medical Identifiers, Codes, and Messages Needed to Create an Efficient Computer-Stored Medical Record

journal articleBiomedical Informatic

The Effect of Natural Dissolved Organic Carbon on the Acute Toxicity of Copper to Larval Freshwater Mussels (\u3cem\u3eGlochidia\u3c/em\u3e)

The present study examined the effect of dissolved organic carbon (DOC), both added and inherent, on Cu toxicity in glochidia, the larvae of freshwater mussels. Using incremental additions of natural DOC concentrate and reconstituted water, a series of acute copper toxicity tests were conducted. An increase in DOC from 0.7 to 4.4 mg C/L resulted in a fourfold increase (36–150 μg Cu/L) in the 24-h median effective concentration (EC50) and a significant linear relationship (r2=0.98, p=0.0008) between the DOC concentration and the Cu EC50 of Lampsilis siliquoidea glochidia. The ameliorating effect of added DOC on Cu toxicity was confirmed using a second mussel species, the endangered (in Canada) Lampsilis fasciola. The effect of inherent (i.e., not added) DOC on Cu toxicity was also assessed in eight natural waters (DOC 5–15 mg C/L). These experiments revealed a significant relationship between the EC50 and the concentration of inherent DOC (r2=0.79, p=0.0031) with EC50s ranging from 27 to 111 μg Cu/L. These laboratory tests have demonstrated that DOC provides glochidia with significant protection from acute Cu toxicity. The potential risk that Cu poses to mussel populations was assessed by comparing Cu and DOC concentrations from significant mussel habitats in Ontario to the EC50s. Although overall mean Cu concentration in the mussel’s habitat was well below the acutely toxic level given the concentration of DOC, episodic Cu releases in low DOC waters may be a concern for the recovery of endangered freshwater mussels. The results are examined in the context of current Cu water quality regulations including the U.S. Environmental Protection Agency’s (U.S. EPA) biotic ligand model

Genomic risk factors in sudden infant death syndrome

Sudden infant death syndrome (SIDS) is a major contributor to postneonatal infant death, and is the third leading cause of infant mortality in the USA. While public health efforts have reduced these deaths in recent years, the pathogenesis of SIDS remains unclear. Epidemiological data on SIDS-related deaths have suggested genetic factors, and many studies have attempted to identify SIDS-associated genes. This has resulted in a large body of literature implicating various genes and their encoded proteins and signaling pathways in numerous cohorts of various sizes and ethnicities. This review has undertaken a systematic evaluation of these studies, identifying the pathways that have been implicated in these studies, including central nervous system pathways, cardiac channelopathies, immune dysfunction, metabolism/energy pathways, and nicotine response. This review also explores how new genomic techniques will aid in advancing our knowledge of the genomic risk factors associated with SIDS, including SNPs and copy number variation. Last, this review explores how the current information can be applied to aid in our assessment of the at risk infant population

Optimization of DNA extraction from human urinary samples for mycobiome community profiling.

IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an expansive look at these communities, identifying both common commensals and fastidious organisms. A fundamental challenge has been the isolation of DNA representative of the entire resident microbial community, including fungi.Materials and methodsWe evaluated multiple modifications of commonly-used DNA extraction procedures using standardized male and female urine samples, comparing resulting overall, fungal and bacterial DNA yields by quantitative PCR. After identifying protocol modifications that increased DNA yields (lyticase/lysozyme digestion, bead beating, boil/freeze cycles, proteinase K treatment, and carrier DNA use), all modifications were combined for systematic confirmation of optimal protocol conditions. This optimized protocol was tested against commercially available methodologies to compare overall and microbial DNA yields, community representation and diversity by next-generation sequencing (NGS).ResultsOverall and fungal-specific DNA yields from standardized urine samples demonstrated that microbial abundances differed significantly among the eight methods used. Methodologies that included multiple disruption steps, including enzymatic, mechanical, and thermal disruption and proteinase digestion, particularly in combination with small volume processing and pooling steps, provided more comprehensive representation of the range of bacterial and fungal species. Concentration of larger volume urine specimens at low speed centrifugation proved highly effective, increasing resulting DNA levels and providing greater microbial representation and diversity.ConclusionsAlterations in the methodology of urine storage, preparation, and DNA processing improve microbial community profiling using culture-independent sequencing methods. Our optimized protocol for DNA extraction from urine samples provided improved fungal community representation. Use of this technique resulted in equivalent representation of the bacterial populations as well, making this a useful technique for the concurrent evaluation of bacterial and fungal populations by NGS