Cost-benefit Analysis of a Genetic Marker on Cow-calf Operations Differentiated by Pasture and Breed

Genetic sequencing in beef cattle (Bos taurus L.) is expected to aid producers with selecting breeding stock. Using data from experimental trials conducted with Angus, Brahman, and their reciprocal cross, the single nucleotide polymorphism (SNP) P450 C994G marker expression was investigated for use in selecting genetics suited to grazing endophyte-infected tall fescue (Festuca arundinacea Schreb. L.) compared to bermudagrass (Cynodon dactylon L.) pasture. The study is unique in the sense that actual cow-calf breeding failure rates (open cows were not culled) were tracked from 1991 to 1997 on herds that were bred to calf in spring and were either exposed to fungal endophyte-infected (Acremonium coenophialum L.) tall fescue grazing and hay or not. The study used the Forage and Cattle Analysis and Planning (FORCAP) decision support software to assess economic performance driven by birth weight, weaning weight, and breeding failure rate differences across treatment. Results suggest that for reciprocal cross herds primarily grazing bermudagrass pastures, the P450 C994C genotype (CC) was most favorable; whereas, the P450 G994C genotype (GC) was more profitable with tall fescue. Adding genetic market information when selecting a production strategy led to approximately $15/head in added profitability. In comparison to the prorated cost of$2.40/head over the life of a dam, the collection, interpretation, and management of genetic information under the conditions observed in this study may be worthwhile

Warassa

1. One of three rulers of Eshnunna between the better attested Ipiq-Adad* I and Ibal-pi-EL I. 2.King of Der, contemporary of Hammurapi of Babylon

Segregation of cortical head direction cell assemblies on alternating theta cycles

High-level cortical systems for spatial navigation, including entorhinal grid cells, critically depend on input from the head direction system. We examined spiking rhythms and modes of synchrony between neurons participating in head direction networks for evidence of internal processing, independent of direct sensory drive, which may be important for grid cell function. We found that head direction networks of rats were segregated into at least two populations of neurons firing on alternate theta cycles (theta cycle skipping) with fixed synchronous or anti-synchronous relationships. Pairs of anti-synchronous theta cycle skipping neurons exhibited larger differences in head direction tuning, with a minimum difference of 40 degrees of head direction. Septal inactivation preserved the head direction signal, but eliminated theta cycle skipping of head direction cells and grid cell spatial periodicity. We propose that internal mechanisms underlying cycle skipping in head direction networks may be critical for downstream spatial computation by grid cells.We kindly thank S. Gillet, J. Hinman, E. Newman and L. Ewell for their invaluable consultations and comments on previous versions of this manuscript, as well as M. Connerney, S. Eriksson, C. Libby and T. Ware for technical assistance and behavioral training. This work was supported by grants from the National Institute of Mental Health (R01 MH60013 and MH61492) and the Office of Naval Research Multidisciplinary University Research Initiative (N00014-10-1-0936). (R01 MH60013 - National Institute of Mental Health; MH61492 - National Institute of Mental Health; N00014-10-1-0936 - Office of Naval Research Multidisciplinary University Research Initiative)Accepted manuscrip

Thermodynamic Studies of High Temperature Materials Via Knudsen Cell Mass Spectrometry

The Knudsen Cell technique is a classic technique from high temperature chemistry for studying condensed phase/vapor equilibria. It is based on a small enclosure, usually about 1 cm in diameter by 1 cm high, with an orifice of well-defined geometry. This forms a molecular beam which is analyzed with mass spectrometry. There are many applications to both fundamental and applied problems with high temperature materials. Specific measurements include vapor pressures and vapor compositions above solids, activities of alloy components, and fundamental gas/solid reactions. The basic system is shown. Our system can accommodate a wide range of samples, temperatures, and attachments, such as gas inlets. It is one of only about ten such systems world-wide

Acute responses to estradiol replacement in the olfactory system of apoE-deficient and wild-type mice

Epidemiological studies suggest that estrogen therapy protects against clinical expression of chronic neurological diseases. These beneficial effects of estrogen therapy are highly modified by apolipoprotein E (apoE) through an unknown mechanism. We examined the short-term effects of estradiol replacement in ovariectomized mice on apoE expression and markers for cell proliferation, reactive gliosis, neuronal maturation, and synaptogenesis in the primary olfactory pathway of wild-type (WT) and apoE knockout (KO) mice. Three days of estradiol replacement increased apoE expression in the olfactory nerve and in the glomerular layer. Estradiol treatment also increased cell proliferation, total cell numbers, number of mature neurons in the olfactory epithelium, and reactive astrocyte numbers in the olfactory bulb (OB) in both WT and KO mice. We also found that estradiol increased glomerular synaptophysin (Syn), but the magnitude of increase was potentiated by the presence of apoE. This data suggest that apoE may be necessary to elicit the complete effect of estradiol on Syn upregualtion

Acute responses to estradiol replacement in the olfactory system of apoE-deficient and wild-type mice

Epidemiological studies suggest that estrogen therapy protects against clinical expression of chronic neurological diseases. These beneficial effects of estrogen therapy are highly modified by apolipoprotein E (apoE) through an unknown mechanism. We examined the short-term effects of estradiol replacement in ovariectomized mice on apoE expression and markers for cell proliferation, reactive gliosis, neuronal maturation, and synaptogenesis in the primary olfactory pathway of wild-type (WT) and apoE knockout (KO) mice. Three days of estradiol replacement increased apoE expression in the olfactory nerve and in the glomerular layer. Estradiol treatment also increased cell proliferation, total cell numbers, number of mature neurons in the olfactory epithelium, and reactive astrocyte numbers in the olfactory bulb (OB) in both WT and KO mice. We also found that estradiol increased glomerular synaptophysin (Syn), but the magnitude of increase was potentiated by the presence of apoE. This data suggest that apoE may be necessary to elicit the complete effect of estradiol on Syn upregualtion