Introduction to Library Trends 56 (1) Summer 2007: Preserving Cultural Heritage

published or submitted for publicatio

Results and prospects on registration of reflected Cherenkov light of EAS from cosmic particles above 10^{15} eV

We give an overview of the SPHERE experiment based on detection of reflected Vavilov-Cherenkov radiation (Cherenkov light) from extensive air showers in the energy region E>10^{15} eV. A brief history of the reflected Cherenkov light technique is given; the observations carried out with the SPHERE-2 detector are summarized; the methods of the experimental datasample analysis are described. The first results on the primary cosmic ray all-nuclei energy spectrum and mass composition are presented. Finally, the prospects of the SPHERE experiment and the reflected Cherenkov light technique are given.Comment: 4 pages, 3 figures, Proc. PANIC-201

Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex

Viral genetic tools to target specific brain cell types in humans and non-genetic model organisms will transform basic neuroscience and targeted gene therapy. Here we used comparative epigenetics to identify thousands of human neuronal subclass-specific putative enhancers to regulate viral tools, and 34% of these were conserved in mouse. We established an AAV platform to evaluate cellular specificity of functional enhancers by multiplexed fluorescent in situ hybridization (FISH) and single cell RNA sequencing. Initial testing in mouse neocortex yields a functional enhancer discovery success rate of over 30%. We identify enhancers with specificity for excitatory and inhibitory classes and subclasses including PVALB, LAMP5, and VIP/LAMP5 cells, some of which maintain specificity in vivo or ex vivo in monkey and human neocortex. Finally, functional enhancers can be proximal or distal to cellular marker genes, conserved or divergent across species, and could yield brain-wide specificity greater than the most selective marker genes