Bacillus atrophaeus MFDV2 RHIZOBACTERIA ISOLATE INCREASES VEGETATIVE GROWTH, YIELD, AND FRUIT SIZE OF BANANA PLANT

Plant growth-promoting rhizobacteria have important roles in soil fertility and have been manipulated for ecologically friendly crop production. The study aimed to determine the effects of a biological agent (Bacillus atrophaeus MFDV2) on the growth, yield, and quality of the banana plant. The study was conducted in 2017–2018 using the banana Dwarf Cavendish clone. Bacterial isolate was cultured on nutrient agar and stored at 30 °C for 24 hours. The culture was then suspended in 0.1 M phosphate buffer to 109 CFU ml-1. The bacterial suspension was applied as foliar, soil and foliar + soil once every two months starting from February. One liter of suspension was applied on the soil and over the leaves per plant. The results showed that the effect of bacterial treatment did not increase stem circumference and leaf length, but it was a significant increase in plant height, leaf number, and leaf width. The effects of bacterial application on banana bunch weight and fruit growth were statistically significant. All applications significantly increased the weight, with the highest increase occurring in foliar finger + soil application. While the effects on the finger diameter were statistically similar. Application on leaf + root has been the biggest increase in finger length. The effects of bacterial applications increased N, P, and K contents in the leaves. The study concluded that the application of Bacillus atrophaeus MFDV2 increased the vegetative growth and fruit quality of the banana plant, so it is advisable to be used in organic banana farming

Single-step approach for synthesis of a novel tetracyclic skeleton: Investigation of X-ray analysis, fluorescence spectra, TD-DFT calculations and biological activities

MakaleWOS:000904967000006Tetracyclic molecules show important properties such as biological activities and fluorescence sensors. Hence, in this study, we have reported unknown tetracyclic skeleton. N -propargylated C-2 and C-4 disubstituted imidazole derivatives were reacted by 2-aminomethyl piperidine without using any transition metal. The reaction unveiled 16 different imidazo[1,2-a]pyrido[1 ' ,2 ' :3,4]imidazo[2,1-c]pyrazine skeletons. Cyclization reaction tolerated phenyl, naphthyl, bi-phenyl, 2-thienyl, and many substituents on the benzene ring such as OMe, CF 3 , and halogens. We have uncovered the exact structure of the tetracyclic skeleton using single-crystal X-ray analysis. Significant fluorescence emission of tetracyclic molecules was investigated, and derivatives bearing electron-withdrawing groups, CF 3 , and the 2-thienyl group possessed a bathochromic effect (shift to longer wavelength) which was confirmed by not only steady-state experiments but HOMOs and LUMOs calculations. The antimicrobial effects of the synthesized molecules on six different or ganisms were tested. Considerable activities of molecules 5p and 5n on many organisms were determined. Docking modeling and Lipinski, Ghose, and Veber compatibility studies of two different molecules were performed.Scientific and Technological Research Council of Türkiye (TUBITAK

Influence of bacterial inoculation on growth and plant nutrition of peach grafted in different rootstocks in calcareous soil

The highly calcareous soil limits plant growth parameters due to inadequate uptake of plant nutrients. Calcareous soil conditions affect plant growth through impaired chlorophyll synthesis, root growth, enzyme synthesis, and nutrient uptake. To overcome the negative effect of calcareous soil, six bacterial strains namely Alcaligenes 637Ca, Agrobacterium A18, Staphylococcus MFDCa1, Staphylococcus MFDCa2, Bacillus M3, and Pantoea FF1 were inoculated in one-year-old plants of peach cultivar ‘Elegant Lady’ grafted onto GF677 and Nemaguard rootstocks. The bacterial treatments were observed to improve plant growth and nutrient content compared to the control. Moreover, the GF677 rootstock was observed to be more tolerant to high calcareous soil conditions than Nemaguard, showing better plant growth and nutrient content. At the Nemaguard rootstocks, the largest leaf area was observed to be upon inoculation with MFDCa2 (29.1 cm2), FF1 (28.8 cm2), and M3 (28.1 cm2), whereas at the GF677 rootstock, the highest leaf area was observed upon inoculation with MFDCa1 (34.7 cm2), FF1 (32.6 cm2), and 637Ca (31.5 cm2). The leaf iron content was higher in bacterial treatments than the control. In the Nemaguard rootstock, the highest iron content was measured in plants inoculated with 637Ca (133.49 mg kg–1) and M3 (127.64 mg kg–1), whereas in the GF677 rootstock, the treatments MFDCa1 (131.51 mg kg–1), 637Ca (131.21 mg kg–1), FF1 (127.72 mg kg–1), and M3 (127.68 mg kg–1) resulted in high iron content. The results indicate that bacterial inoculations have a significant potential to improve plant growth and can be used as biofertilizers for peach grafted onto Nemaguard and GF677 in high calcareous soil conditions

The Effects of PGPR Treatments on Plant Growth of Deveci Pear Cultivar in Calcareous Soil Condition

Bu çalışma 2012-2015 yılları arasında Selçuk üniversitesi Ziraat Fakültesi Bahçe Bitkileri Bölümü araştırma veuygulama arazisinde yürütülmüştür. Araştırmada 6 farklı bakteri ırkının (Alcaligenes 637Ca, Agrobacterium A18,Staphylococcus MFDCa1, MFDCa2, Bacillus M3 ve Pantoea FF1) OHF333 ve BA 29 anacı üzerine aşılı Deveci armutçeşidinde bitki gelişimi üzerine etkisi araştırılmıştır. Bakteri ırklarının yaprak alanı üzerine etkisi incelendiğinde en genişyaprak alanı OHF333 anacı üzerinde 16.1cm2 ile FF1 bakteri ırkından elde edilirken, BA 29 anacı üzerinde ise 15.8cm2637Ca bakteri ırkından elde edilmiştir. Bakterilerin anaç çapı üzerine etkisi ele alındığında en iyi sonuçlar OHF333anacında MFDCa1 bakteri ırkından (30.1mm), BA29 anacında ise 637Ca ve A18 bakteri ırklarından (28.3mm ve28.1mm) elde edilmiştir. Bakteri uygulamalarının sürgün uzunluğuna etkisi her iki anaçta da oldukça farklılık yaratmışolup OHF333 anacında en iyi sonuç 6.68cm ve 5.95cm ile MFDCa1 ile 637Ca olurken, BA29 anacında ise 637Ca(26.7cm) en iyi sonuca sahip olmuştur. Yapraklardaki klorofil miktarı bakımından OHF333 anacında en yüksek değerMFDCa2 bakteri ırkında (48.8 SPAD değeri) ölçülürken, BA29 anacında ise MFDCa2 (45.7 SPAD değeri) ile M3 (43.7SPAD değeri) bakteri ırklarından ölçülmüştür.This study was conducted in Research field of University of Selçuk, Faculty of Agriculture and HorticultureDepartment in 2012-2015 years. The effect of six bacteria strain (Alcaligenes 637Ca, Agrobacterium A18,Staphylococcus MFDCa1, MFDCa2, Bacillus M3 and Pantoea FF1) were investigated on plant growth of “Deveci”cultivar grafted on OHF333 and BA29 rootstocks. The FF1 bacteria strain increased leaf area 16.1cm2 on OHF333rootstock while leaf area was increased 15.8 cm2 by 637Caon BA29 rootstock. The rootstock diameter was increased byMFDCa1 strain with 30.1mm on OHF333 while 637Ca and A18 increased rootstock diameter on BA29 28.3mm and28.1mm respectively. Lateral branch length was found 6.68 cm and 5.95 cm with MFDCa1 and 637Ca on OHF333. InBA29 rootstock, 637Ca increased lateral branch length at 26.7cm. The chlorophyll content was measured 48.8 SPADUnits by MFDCa2 strain on OHF333 while MFDCa2 and M3 had the good result in BA29 45.7 and 43.7 SPAD Unitsrespectively