14 research outputs found

    Uso de indicadores de sustentabilidade na gestão de projetos de tecnologia da informação

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    Purpose - To understand the relationship between sustainability indicators and information technology projects.Design/methodology/approach – This is an exploratory study with a qualitative approach of multiple cases involving service providers in the information technology sector.Findings – It was found that companies use sustainability indicators at an organizational level for management of information technology projects, but no specific sustainability indicator was present in none of the cases. Thus, the use of sustainability indicators depends on the nature of the information technology project.Research limitations/implications - This study is limited to the identification of sustainability indicators in information technology companies.Originality/value - Although this study points to the effectiveness of the use of sustainability indicators in projects, there is still a gap for performing new studies on information technology projects with the objective of increasing the companies’ economic performance.Objetivo – O objetivo deste estudo é compreender a relação entre indicadores de sustentabilidade e projetos do segmento de Tecnologia da Informação.Metodologia - A pesquisa caracteriza-se como exploratória com abordagem qualitativa realizada por meio de um estudo de caso múltiplo realizado em prestadores de serviços da área de Tecnologia da Informação.Resultados – A pesquisa concluiu que as organizações não possuem a aplicação de indicadores de sustentabilidade em nível organizacional na gestão de projetos de Tecnologia da Informação, porém, nenhum dos casos abordados apresenta a presença de indicadores específicos para projetos de Tecnologia da Informação. Assim, a utilização de indicadores de sustentabilidade está sujeita à natureza envolvida no projeto de tecnologia da informação.Limitações/implicações da pesquisa - O estudo se limita à identificação de indicadores de sustentabilidade em empresas de tecnologia da informação.Originalidade - Embora pesquisas apontem para a eficácia da utilização de indicadores de sustentabilidade em projetos, ainda existe uma lacuna para a realização de novos estudos em projetos de tecnologia da informação, com o objetivo de aumentar seu desempenho econômico

    Infection with <i>T. rangeli</i> reduces the NOS activity and the levels of NOS protein in the salivary glands of <i>R. prolixus</i>.

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    <p>A. <i>Rhodnius</i> were dissected 7 days after control injection of water or <i>T. rangeli</i> and assayed for NADPH-diaphorase activity. Results from three experiments were evaluated statistically using the Student t test (* p<0.05). B. Salivary gland extracts from control or <i>T. rangeli-</i>injected insects were fractionated by SDS-PAGE and transferred to a nitrocellulose membrane. The membranes were incubated with primary antibody anti-NOS and then with an anti-rabbit antibody conjugated to alkaline phosphatase. This experiment was performed three times. Tr, <i>Trypanosoma rangeli</i> cells evalutated for NOS blotting. N, salivary glands from non-injected insects. C, control salivary glands from insects injected with water. I, Salivary glands from <i>T. rangeli</i>-injected insects. C. NADPH-diaphorase activity was measured in salivary gland extracts of salivary glands three days after injection with 100 ng of glycolipids from either <i>T. rangeli</i> (Tr GIPL), <i>P. serpens (</i>Ps GIPL<i>)</i> or <i>T. cruzi</i> eGPI-mucin (Tc Mucin). The experiment was performed three times and analyzed by ANOVA (* p<0.05).</p

    Glycolipid-mediated suppression of NO synthesis occurs through the manipulation of intracellular phosphorylation-dephosphorylation circuits.

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    <p>Intracellular circuits of protein-phosphorylation and dephosphorylation were evaluated through different assays. A. Salivary glands obtained from either control or <i>T. rangeli</i>-infected insects were dissected three days after the injection, homogeneized and phosphorylated in the presence of <sup>32</sup>P-ATP followed by SDS-gel electrophoresis and autoradiograph. B. A similar experiment was conducted with salivary glands isolated from insects injected with Tr GIPL or Tc GIPL. C. Following a blood meal on rabbit ear salivary glands were dissected at different points in time. Total protein phosphatase activity was followed during the refilling cycle of salivary glands using pNPP as substrate. Data is the mean ± S.E. of three different experiments. D. Insects were injected with Tr GIPL and evaluated for protein phosphatase activity in the presence and in the absence of SO. The fraction of enzyme activity inhibited by SO in control and Tr GIPL-injected insects is shown. Data is the mean ± S.E. of three different experiments.</p

    <i>T. rangeli</i> infection downregulates NOS synthesis.

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    <p><i>Rhodnius</i> were infected with <i>T. rangeli</i> and three days later salivary glands were dissected and incubated in the presence of the NO fluorescent probe DAF-FM. A, C are contrast-phase imaging of B and D, respectively. A. Control salivary gland. B. DAF-FM fluorescent image of a control salivary gland shown on A. C. Infected salivary gland. D. DAF-FM fluorescence image of an infected salivary gland shown on C.</p

    NADPH-diaphorase activity of NOS in <i>Rhodnius prolixus</i> salivary glands after a blood meal and the expression of NOS.

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    <p>A. Salivary glands were dissected in different days after blood feeding and evaluated for NOS NAPDH-diaphorase activity. Salivary glands were assayed in 10 mM Tris-HCl pH 8,0, 0,05 M NaCl, 0,1%, Triton X-100, 1 mM CaCl<sub>2</sub>, 5 µM FAD, 1 mM NADPH and 0,5 mg/mL MTT. MTT reduction was followed at 540 nm for 30 min at 37°C. Also samples were obtained and NOS content evaluated by Western blotting. Each point is the average and SE of 05 different experiments. B. Immunoblotting using an anti-NOS antibody. Blottings were developed with the use of a secondary antibody conjugated to alkaline phosphatase in the presence of the substrate Western Blue. Molecular mass markers are indicated at the left. C. Upper panel<b>,</b> total RNA from the salivary glands at different days after feeding was isolated and cDNA was synthesized. Samples were then analyzed by semi-quantitative PCR with temperatures of 55, 72 and 94°C for 27 cycles with primers for NOS. Lower panel, analysis of 18 S RNA levels. In this case reaction occurred for 25 cycles. The products of reactions shown on panels C were separated on agarose gel 1.4% stained with ethidium bromide and photographed under ultraviolet light. Molecular mass standards are indicated at the left.</p

    Tc GIPL does not affect regular blood feeding, anti-clotting and apyrase activity.

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    <p><i>Rhodnius</i> injected or not with Tc GIPLs were evaluated for their ability to feed on blood. Parallel controls in each panel were obtained in insects inject with GIPL solvent. Three days after the injection insects were either allowed to feed on a rabbit ear or their salivary glands were dissected and evaluated for anti-hemostatic activities. A. Weight gain after blood feeding. B. Apyrase activity. C. aPTT activity. Data is the mean ± S.E. of three different experiments.</p
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