Классицизм – основа формирования архитектуры Красноярска 1800–1850-х годов

The article tells how Classicism played the leading role in formation of Krasnoyarsk architecture of the 1800-1850s. The stylistic standards of Classicism were mostly demonstrated in stone and wooden houses built according to the model projects in downtown neighborhoods. The author concludes that the civil architecture of Krasnoyarsk developed until the 1850s within the frameworks of the Classicism stylistic system, while new tendencies occurred very rarely.Показано, что ведущее значение в формировании архитектуры Красноярска 1800–1850-х годов принадлежит классицизму. В наибольшей степени стилевые нормы классицизма проявились в облике каменных и деревянных жилых зданий, возводившихся в соответствии с образцовыми проектами, в центральных городских кварталах. Сделан вывод о том, что гражданская архитектура Красноярска до конца 1850-х годов продолжала развиваться в рамках стилевой системы классицизма, проявление новых тенденций было незначительным

Колебательно-вращательный анализ спектров высокого разрешения фундаментальной полосы v3 диоксида хлора 16O 35Cl16O

С использованием Фурье-спектрометра Bruker IFS 125 HR была зарегистрирована колебательно-вращательная структура полосы ν3 молекулы ClO2 с разрешением 0,0015 см-1. Данная полоса была проанализирована с использованием улучшенной модели эффективного Гамильтониана и разработанной новой компьютерной программой для анализа колебательно-вращательных спектров молекул свободных радикалов с открытой оболочкой, учитывающей спин-вращательные взаимодействия. Более 4200 переходов, принадлежащих данной полосе, были проинтерпретированы с максимальными значениями Nmax=68 и Ka max=21, что впоследствии было использовано для определения набора из 13 спектроскопических параметров исследуемого колебательного состояния. Среднеквадратичное отклонение составило drms=2,4·10-4 см -1

Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO

AbstractWe have previously shown that the V-ATPase a2-subunit isoform interacts specifically, and in an intra-endosomal acidification-dependent manner, with the Arf-GEF ARNO. In the present study, we examined the molecular mechanism of this interaction using synthetic peptides and purified recombinant proteins in protein-association assays. In these experiments, we revealed the involvement of multiple sites on the N-terminus of the V-ATPase a2-subunit (a2N) in the association with ARNO. While six a2N-derived peptides interact with wild-type ARNO, only two of them (named a2N-01 and a2N-03) bind to its catalytic Sec7-domain. However, of these, only the a2N-01 peptide (MGSLFRSESMCLAQLFL) showed specificity towards the Sec7-domain compared to other domains of the ARNO protein. Surface plasmon resonance kinetic analysis revealed a very strong binding affinity between this a2N-01 peptide and the Sec7-domain of ARNO, with dissociation constant KD=3.44×10−7M, similar to the KD=3.13×10−7M binding affinity between wild-type a2N and the full-length ARNO protein. In further pull-down experiments, we also revealed the involvement of multiple sites on ARNO itself in the association with a2N. However, while its catalytic Sec7-domain has the strongest interaction, the PH-, and PB-domains show much weaker binding to a2N. Interestingly, an interaction of the a2N to a peptide corresponding to ARNO's PB-domain was abolished by phosphorylation of ARNO residue Ser392. The 3D-structures of the non-phosphorylated and phosphorylated peptides were resolved by NMR spectroscopy, and we have identified rearrangements resulting from Ser392 phosphorylation. Homology modeling suggests that these alterations may modulate the access of the a2N to its interaction pocket on ARNO that is formed by the Sec7 and PB-domains. Overall, our data indicate that the interaction between the a2-subunit of V-ATPase and ARNO is a complex process involving various binding sites on both proteins. Importantly, the binding affinity between the a2-subunit and ARNO is in the same range as those previously reported for the intramolecular association of subunits within V-ATPase complex itself, indicating an important cell biological role for the interaction between the V-ATPase and small GTPase regulatory proteins

Mapping the H+ (V)-ATPase interactome: identification of proteins involved in trafficking, folding, assembly and phosphorylation

V-ATPases (H+ ATPases) are multisubunit, ATP-dependent proton pumps that regulate pH homeostasis in virtually all eukaryotes. They are involved in key cell biological processes including vesicle trafficking, endosomal pH sensing, membrane fusion and intracellular signaling. They also have critical systemic roles in renal acid excretion and blood pH balance, male fertility, bone remodeling, synaptic transmission, olfaction and hearing. Furthermore, V-ATPase dysfunction either results in or aggravates various other diseases, but little is known about the complex protein interactions that regulate these varied V-ATPase functions. Therefore, we performed a proteomic analysis to identify V-ATPase associated proteins and construct a V-ATPase interactome. Our analysis using kidney tissue revealed V-ATPase-associated protein clusters involved in protein quality control, complex assembly and intracellular trafficking. ARHGEF7, DMXL1, EZR, NCOA7, OXR1, RPS6KA3, SNX27 and 9 subunits of the chaperonin containing TCP1 complex (CCT) were found to interact with V-ATPase for the first time in this study. Knockdown of two interacting proteins, DMXL1 and WDR7, inhibited V-ATPase-mediated intracellular vesicle acidification in a kidney cell line, providing validation for the utility of our interactome as a screen for functionally important novel V-ATPase-regulating proteins. Our data, therefore, provide new insights and directions for the analysis of V-ATPase cell biology and (patho)physiology

Structural model of a2-subunit N-terminus and its binding interface for Arf-GEF CTH2: Implication for regulation of V-ATPase, CTH2 function and rational drug design

We have previously identified the interaction between mammalian V-ATPase a2-subunit isoform and cytohesin-2 (CTH2) and studied molecular details of binding between these proteins. In particular, we found that six peptides derived from the N-terminal cytosolic domain of a2 subunit (a2N1–402) are involved in interaction with CTH2 (Merkulova, Bakulina, Thaker, Grüber, & Marshansky, 2010). However, the actual 3D binding interface was not determined in that study due to the lack of high-resolution structural information about a-subunits of V-ATPase. Here, using a combination of homology modeling and NMR analysis, we generated the structural model of complete a2N1–402 and uncovered the CTH2-binding interface. First, using the crystal-structure of the bacterial M. rubber Icyt-subunit of A-ATPase as a template (Srinivasan, Vyas, Baker, & Quiocho, 2011), we built a homology model of mammalian a2N1–352 fragment. Next, we combined it with the determined NMR structures of peptides a2N368–395 and a2N386–402 of the C-terminal section of a2N1–402. The complete molecular model of a2N1–402 revealed that six CTH2 interacting peptides are clustered in the distal and proximal lobe sub-domains of a2N1–402. Our data indicate that the proximal lobe sub-domain is the major interacting site with the Sec7 domain of first CTH2 protein, while the distal lobe sub-domain of a2N1–402 interacts with the PH-domain of second CTH2. Indeed, using Sec7/Arf-GEF activity assay we experimentally confirmed our model. The interface formed by peptides a2N1–17 and a2N35–49 is involved in specific interaction with Sec7 domain and regulation of GEF activity. These data are critical for understanding of the cross-talk between V-ATPase and CTH2 as well as for the rational drug design to regulate their function

Features of Window Decorations of Wooden Houses in Krasnoyarsk at the end of XIX - the Beginning of XX Century

The article is devoted to the window casings which are one of the main elements in decoration of timber facades in residential buildings in Krasnoyarsk. The window casing is an important functional and structural element of the facade; it peculiarized and gave an individual shape to every house with its variety of artistic treatments. The study is based on a long-term experience of studying Krasnoyarsk wooden architecture, including on-site investigations such as photofixation and measurements. The article dwells upon specific examples of component parts and ways of decorating window casings. A classification of window castings has been worked out and shown, the main attention is paid to the upper crowning part. The sources of decorative sculpture forms have also been identified. Considerable attention is paid to the process of interaction between national and professional style of architecture, in which architecture styles like baroque, classicism, art nouveau (modernism) found their reflection in the forms and decoration of the window casings.Статья посвящена наличникам окон - одному из главных элементов в системе декора фасадов деревянных жилых домов Красноярска. Оконный наличник - важный функ-циональный и конструктивный элемент фасада, благодаря разнообразной художествен-ной трактовке придавал индивидуальность облику каждого дома. Исследование основы-вается на многолетнем опыте изучения деревянной архитектуры г. Красноярска, в том числе на натурных обследованиях: фотофиксации, обмерах. В статье на конкретных примерах рассмотрены составные части и способы декорирования наличников, разрабо-тана классификация наличников в зависимости от композиции основной - венчающей части. Обозначены источники форм декоративной пластики. Значительное внимание уделено процессу взаимодействия народной и профессиональной стилевой архитектуры, в ходе которого в формах и декоре оконных наличников нашли отражение такие архитектурные стили, как барокко, классицизм, модерн

Features of Window Decorations of Wooden Houses in Krasnoyarsk at the end of XIX - the Beginning of XX Century

The article is devoted to the window casings which are one of the main elements in decoration of timber facades in residential buildings in Krasnoyarsk. The window casing is an important functional and structural element of the facade; it peculiarized and gave an individual shape to every house with its variety of artistic treatments. The study is based on a long-term experience of studying Krasnoyarsk wooden architecture, including on-site investigations such as photofixation and measurements. The article dwells upon specific examples of component parts and ways of decorating window casings. A classification of window castings has been worked out and shown, the main attention is paid to the upper crowning part. The sources of decorative sculpture forms have also been identified. Considerable attention is paid to the process of interaction between national and professional style of architecture, in which architecture styles like baroque, classicism, art nouveau (modernism) found their reflection in the forms and decoration of the window casings.Статья посвящена наличникам окон - одному из главных элементов в системе декора фасадов деревянных жилых домов Красноярска. Оконный наличник - важный функ-циональный и конструктивный элемент фасада, благодаря разнообразной художествен-ной трактовке придавал индивидуальность облику каждого дома. Исследование основы-вается на многолетнем опыте изучения деревянной архитектуры г. Красноярска, в том числе на натурных обследованиях: фотофиксации, обмерах. В статье на конкретных примерах рассмотрены составные части и способы декорирования наличников, разрабо-тана классификация наличников в зависимости от композиции основной - венчающей части. Обозначены источники форм декоративной пластики. Значительное внимание уделено процессу взаимодействия народной и профессиональной стилевой архитектуры, в ходе которого в формах и декоре оконных наличников нашли отражение такие архитектурные стили, как барокко, классицизм, модерн

Formation of properties of meat-containing canned food with the use of rabbit meat for children’s and dietary food

The modern level of knowledge about food products allows them to be developed with specified properties that correspond to a specific purpose. The creation of such products is always a search for a reasonable compromise between the numerous requirements for food products. The article reflects the results of a scientific study devoted to the development of a recipe for canned meat for baby food with a different percentage of rabbit meat and vegetables. Comprehensive studies of the consumer properties of canned food obtained according to one of the author’s recipes were carried out. The obtained data and their analysis made it possible to choose the most optimal option for canned food, which will be in demand and competitive in relation to other types of products in the assortment group