Effect of bicarbonate on uptake and translocation of 59Fe in two grapevine rootstocks differing in their resistance to Fe deficiency chlorosis

In order to study the effect of high bicarbonate concentration in the root medium on root FeIII reduction, Fe uptake and its translocation to the leaves, two rootstocks (Vitis riparia Michx., susceptible, and 41 B (Vitis vinifera L. cv. Chasselas x Vitis berlandieri Planch.), resistant to Fe deficiency chlorosis) were pre-cultivated in nutrient solutions with high and low Fe supply. After three weeks of preculture at low Fe, chlorosis symptoms occurred in both, Fe-resistant and Fe-susceptible genotypes. The FeIII reducing capacity by roots was enhanced at Fe deficiency in both genotypes, which was consistent with the increase of subsequent root uptake and translocation rates of 59Fe. In the presence of bicarbonate in the solutions the FeIII reducing capacity, 59Fe uptake and translocation rate decreased in both genotypes precultured with low re supply. The 59Fe uptake and translocation rate, however, were significantly higher in the Fe chlorosis-resistant rootstock 41 B. These results clearly indicate that bicarbonate-induced Fe chlorosis in grapevine rootstocks is obviously caused by an inhibition of Fe uptake and translocation due to an inhibition of FeIII reduction by root cells. The fact that these processes were less inhibited in the chlorosis-resistant rootstock hints to genotypical differences in Fe acquisition by roots at high bicarbonate levels. These differences might be used in breeding programs to identify Fe chlorosis-resistant rootstocks

A tripartite aseptic culture system for grape (Vitis spp), phylloxera (Daktulosphaera vitifoliae) and mites (Tarsanemus sp.)

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Aseptic dual culture of grape (Vitis spp.) and grape phylloxera (Daktulosphaira vitifoliae FITCH)

An aseptic dual culture of grape phylloxera (Daktulosphaira vitifoliae FITCH) and grape vine (Vitis spp.) was developed. This method permits continuous observation of phylloxera feeding and the whole plant response on a dynamic basis. The plant/parasite interaction of three testplants (V. vinifera L., var. Riesling, SO 4 (V. berlandieri PLANCH. X V. riparia L.) and V. riparia, var. Gloire de Montpellier) are demonstrated by observing post-infectious reactions of the host- and population dynamics of the parasite. Different stages of phylloxera could be observed including nymphs, winged phylloxera (alatae) and sexual male phylloxera. Several potential applications for this aseptic dual culture are demonstrated

Der Stickstoffexport der Wurzel und die Zusammensetzung des Xylemexsudats Teil 1: Der Einfluß einer zunehmenden Stickstoffdüngung

The nitrogen output of the root and the nitrogenous compounds in the xylem exudate.Part 1: The influence of increasing nitrogen fertilizationTwo-year-old two-bud cuttings in pots supplied with two levels of nitrogen (0.5 and 3.0 g N/vine) were tested for dry mass production, N uptake and the N composition in the xylem exudate. The rootstock 125 AA showed the highest dry mass production, Silvaner the lowest one. Both N output of the root (calculated from the N content of the xylem sap) and the N uptake/vine are analogous to dry mass production, the N uptake being 595 resp. 2003 mg N for 125 AA, 245 resp. 1403 mg N for Silvaner and 423 resp. 1363 mg N for C 3309 (N fertilization 0.5 and 3.0 g N/vine, resp.). N is transported within the xylem as NO3-N and NH2-N. The proportion of NO3-N depends much on the N fertilization (4-25 % NO3-N at low and 16-42 % at high N fertilization). The N composition in the xylem exudate does not depend on grape variety. With 66 % glutamine is the main transport form

Effect of soil fertilization on the incidence of berry shrivel and the quality of resulting wine

Berry shrivel is becoming an increasing concern for winegrowers all over the world. Until today, no single factor causing this physiological disorder has been determined. Studies concerning berry shrivel conducted in Austria have shown that an unbalanced ratio of K and Mg in the soil is a likely factor contributing to the disorder. The aims of the present study were to establish a better understanding of the causes and consequences of berry shrivel and observe the effects of K and Mg fertilization via the soil on the incidence of berry shrivel, the mineral composition of affected berries and the resulting wine quality. A two-year fertilization trial was conducted on two sites located within southern Germany with the varieties 'Zweigelt' and 'Pinot Blanc'. Different amounts of K and Mg were applied each year at both locations in order to generate different ratios of K and Mg in the soil. Before harvest, the incidences of berry shrivel of the different treatments were determined. In addition, macronutrients including K, Mg and Ca that were translocated in healthy berries and berries affected by berry shrivel were determined at harvest. To compare the quality of wine influenced by berry shrivel, different wines were produced consisting of shrivelled berries, berries affected by bunch stem necrosis and healthy berries. In the soil fertilization trials, no significant differences in the incidences of berry shrivel were observed in relation to the soil fertilization. Major differences were found in the wine qualities of the different wines. Wines produced from healthy berries were always rated as the best wines, whereas wines produced from shrivelled berries were always rated as the lowest quality. The low quality parameters found in the must did not improve in the wine making process. Wines produced from berries affected by bunch stem necrosis were rated better than berry-shrivel-wines, however, rated less than the wine produced from healthy berries. The determinations of macronutrients’ level in the berries showed significant differences regarding the concentration of Ca. In the variety 'Zweigelt' in 2009, an average of 36 mg∙L-1 of Ca were found in healthy berries and 107 mg∙L-1 in berries affected by berry shrivel. In 'Pinot Blanc' in 2010, the average of Ca in healthy berries was 46 mg∙L-1 and 70 mg∙L-1 in berries affected by berry shrivel. No significant differences were found for K and Mg in the berries.

Critical regime of two dimensional Ando model: relation between critical conductance and fractal dimension of electronic eigenstates

The critical two-terminal conductance $g_c$ and the spatial fluctuations of critical eigenstates are investigated for a disordered two dimensional model of non-interacting electrons subject to spin-orbit scattering (Ando model). For square samples, we verify numerically the relation $\sigma_c=1/[2\pi(2-D(1))] e^2/h$ between critical conductivity $\sigma_c=g_c=(1.42\pm 0.005) e^2/h$ and the fractal information dimension of the electron wave function, $D(1)=1.889\pm 0.001$. Through a detailed numerical scaling analysis of the two-terminal conductance we also estimate the critical exponent $\nu=2.80\pm 0.04$ that governs the quantum phase transition.Comment: IOP Latex, 7 figure

Alternative pathways of dewetting for a thin two-layer film of soft matter

We consider two stacked ultra-thin layers of different liquids on a solid substrate. Using long-wave theory, we derive coupled evolution equations for the free liquid-liquid and liquid-gas interfaces. Linear and non-linear analyses show that depending on the long-range van-der-Waals forces and the ratio of the layer thicknesses, the system follows different pathways of dewetting. The instability may be driven by varicose or zigzag modes and leads to film rupture either at the liquid-gas interface or at the substrate

Dissociation energy of the hydrogen molecule at 10−9^{-9} accuracy

The ionization energy of ortho-H$_2$ has been determined to be $E^\mathrm{o}_\mathrm{I}(\mathrm{H}_2)/(hc)=124\,357.238\,062(25)$ cm$^{-1}$ from measurements of the GK(1,1)--X(0,1) interval by Doppler-free two-photon spectroscopy using a narrow band 179-nm laser source and the ionization energy of the GK(1,1) state by continuous-wave near-infrared laser spectroscopy. $E^\mathrm{o}_\mathrm{I}$(H$_2$) was used to derive the dissociation energy of H$_2$, $D^{N=1}_{0}$(H$_2$), at $35\,999.582\,894(25)$ cm$^{-1}$ with a precision that is more than one order of magnitude better than all previous results. The new result challenges calculations of this quantity and represents a benchmark value for future relativistic and QED calculations of molecular energies.Comment: 6 pages, 5 figure

Determination of the Interval between the Ground States of Para- and Ortho- H2

ISSN:0031-9007ISSN:1079-711