Effects of an Interview Guide on the Accuracy of Ratings for Applicants with Disabilities

The problem of bias in the employment interview for applicants with disabilities was addressed with research to identify if a decision aid can increase the decision making accuracy of interviewers. A survey designed to allow participants to rate applicants with five disabilities for three jobs (with three essential functions listed for each job) was used to assess rating accuracy of two groups. Participants who received the decision aid in the form of a Guide to Interviewing People with Selected Disabilities were expected to have more rating accuracy than those participants without access to the Guide. Accuracy was assessed by comparing participant ratings to target scores generated by an expert panel. Participants who received the Guide did not make more accurate ratings than the participants who completed the survey without access to the Guide, but it is likely that the results are a function of the limitations of the training rather than the Guide. Raters were significantly less accurate when rating the applicant with multiple sclerosis, as hypothesized. However, raters were also significantly less accurate for the applicant with a hearing impairment, despite their familiarity with the disability. The significantly lenient rating may be a function of the raters not considering the intense hearing requirements of the job tasks as seriously as did the experts. The practical implications for these findings are discussed with respect to interviewing applicants with disabilities

The effect of membrane thickness on the membrane permeabilizing activity of the cyclic lipopeptide tolaasin II

Tolaasin II is an amphiphilic, membrane-active, cyclic lipopeptide produced by Pseudomonas tolaasii and is responsible for brown blotch disease in mushroom. To better understand the mode of action and membrane selectivity of tolaasin II and related lipopeptides, its permeabilizing effect on liposomes of different membrane thickness was characterized. An equi-activity analysis served to distinguish between the effects of membrane partitioning and the intrinsic activity of the membrane-bound peptide. It was found that thicker membranes require higher local peptide concentrations to become leaky. More specifically, the mole ratio of membrane-bound peptide per lipid needed to induce 50% leakage of calcein within 1 h, R-e (50), increased monotonically with membrane thickness from 0.0016 for the 14:1 to 0.0070 for the 20:1 lipid-chains. Moreover, fast but limited leakage kinetics in the low-lipid regime were observed implying a mode of action based on membrane asymmetry stress in this time and concentration window. While the assembly of the peptide to oligomeric pores of defined length along the bilayer z-axis can in principle explain inhibition by increasing membrane thickness, it cannot account for the observed limited leakage. Therefore, reduced intrinsic membrane-permeabilizing activity with increasing membrane thickness is attributed here to the increased mechanical strength and order of thicker membranes