Сравнительная оценка влияния сочетанного применения препаратов Фелиферон и Азоксивет и препарата Глобфел-4 при ринотрахеите кошек на гематологические показатели белой крови

The comparative effect of the combined drugs Feliferon and Azoksivet and the drug Globfel-4 for rhinotracheitis in felines on hematological parameters of white blood was studied. In our study on the treatment of viral rhinotracheitis, 16 cats were examined. During the experiment, all cats showed the following clinical signs: lethargy, fever, leakage from the eyes and nasal passages. The animals were kept in separate cages in accordance with the European Convention for the Protection of Vertebrates. The control group was given 1 ml subcutaneously, once a day every other day, with a course of 7 injections, immunoglobulin against rhinotracheitis, calicivirus and chlamydia - Globfel-4. The experimental group was treated with drugs Azoxivet intramuscularly in a course of 7 injections, once a day every other day, and Feliferon at a dose of 400,000 IU intramuscularly, once a day. The course of treatment was 7 days. Blood sampling was performed from the anterior saphenous vein of the forearm before and after therapy. The concentration of erythrocytes and hemoglobin was determined on an automatic hematology analyzer Mindray BC-2800. When treating cats with rhinotracheitis with Globfel-4, pronounced leukocytosis, absolute and relative lymphocytosis were noted. With the combined use of Azoxivet and Feliferon, these blood changes do not occur. When treating cats with rhinotracheitis with Globfel-4 or the combined use of Azoxivet and Feliferon, the absolute concentrations of monocytes and granulocytes increase within the physiological norm. The relative concentrations of these white blood phrases increase within the physiological norm only with the combined use of Azoxivet and Feliferon.Изучалось сравнительное влияние сочетанного препаратов Фелиферон и Азоксивет и препарата Глобфел-4 при ринотрахеите кошек на гематологические показатели белой крови. В нашем исследовании по изучению методов лечения вирусного ринотрахеита было обследовано 16 кошек. У всех кошек в период проведения опыта выявлены следующие клинические признаки: вялость, повышение температуры, истечение из глаз и носовых путей. Животные содержались в отдельных клетках в соответствии с Европейской конвенцией по защите позвоночных. Контрольной группе поставили подкожно по 1 мл, 1 раз в сутки через день, курсом 7 введений, иммуноглобулин против ринотрахеита, калицивироза и хламидиоза – Глобфел-4. Опытную группу лечили препаратами Азоксивет – внутримышечно курсом 7 введений, 1 раз в сутки через день, и Фелиферон в дозе 400 000 МЕ внутримышечно, 1 раз в день, курс лечения 7 дней. Забор крови проводили из передней подкожной вены предплечья до начала терапии и после. Концентрацию эритроцитов и гемоглобина определяли на гематологическом автоматическом анализаторе Mindray BC-2800. При терапии кошек с ринотрахеитом препаратом Глобфел-4 отмечается выраженный лейкоцитоз, абсолютный и относительный лимфоцитоз. При сочетанном применении Азоксивета и Фелиферона данных изменений крови не происходит. При терапии кошек с ринотрахеитом препаратом Глобфел-4 или сочетанным применением препаратов Азоксивет и Фелиферон повышаются в пределах физиологической нормы абсолютные концентрации моноцитов и гранулоцитов. Относительные концентрации этих фраций белой крови возрастают в пределах физиологической нормы только при сочетанном применении препаратов Азоксивет и Фелиферон

A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale

In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is however critical both for basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brain-wide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brain-wide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open access data repository; compatibility with existing resources, and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.Comment: 41 page

Fine-Tuning and the Stability of Recurrent Neural Networks

A central criticism of standard theoretical approaches to constructing stable, recurrent model networks is that the synaptic connection weights need to be finely-tuned. This criticism is severe because proposed rules for learning these weights have been shown to have various limitations to their biological plausibility. Hence it is unlikely that such rules are used to continuously fine-tune the network in vivo. We describe a learning rule that is able to tune synaptic weights in a biologically plausible manner. We demonstrate and test this rule in the context of the oculomotor integrator, showing that only known neural signals are needed to tune the weights. We demonstrate that the rule appropriately accounts for a wide variety of experimental results, and is robust under several kinds of perturbation. Furthermore, we show that the rule is able to achieve stability as good as or better than that provided by the linearly optimal weights often used in recurrent models of the integrator. Finally, we discuss how this rule can be generalized to tune a wide variety of recurrent attractor networks, such as those found in head direction and path integration systems, suggesting that it may be used to tune a wide variety of stable neural systems

To the Cloud! A Grassroots Proposal to Accelerate Brain Science Discovery

The revolution in neuroscientific data acquisition is creating an analysis challenge. We propose leveraging cloud-computing technologies to enable large-scale neurodata storing, exploring, analyzing, and modeling. This utility will empower scientists globally to generate and test theories of brain function and dysfunctio

Young man with destructive perioral lesion and oral incontinence.

<p>Photo courtesy of Brian L. Fisher, California Academy of Sciences.</p

Spontaneous eye movements in goldfish: oculomotor

integrator performance, plasticity, and dependence on visual feedbac