Enhancing Cyber-Resiliency of DER-based SmartGrid: A Survey

The rapid development of information and communications technology has enabled the use of digital-controlled and software-driven distributed energy resources (DERs) to improve the flexibility and efficiency of power supply, and support grid operations. However, this evolution also exposes geographically-dispersed DERs to cyber threats, including hardware and software vulnerabilities, communication issues, and personnel errors, etc. Therefore, enhancing the cyber-resiliency of DER-based smart grid - the ability to survive successful cyber intrusions - is becoming increasingly vital and has garnered significant attention from both industry and academia. In this survey, we aim to provide a systematical and comprehensive review regarding the cyber-resiliency enhancement (CRE) of DER-based smart grid. Firstly, an integrated threat modeling method is tailored for the hierarchical DER-based smart grid with special emphasis on vulnerability identification and impact analysis. Then, the defense-in-depth strategies encompassing prevention, detection, mitigation, and recovery are comprehensively surveyed, systematically classified, and rigorously compared. A CRE framework is subsequently proposed to incorporate the five key resiliency enablers. Finally, challenges and future directions are discussed in details. The overall aim of this survey is to demonstrate the development trend of CRE methods and motivate further efforts to improve the cyber-resiliency of DER-based smart grid.Comment: Submitted to IEEE Transactions on Smart Grid for Publication Consideratio

Dual Supramolecular Nanoparticle Vectors Enable CRISPR/Cas9-Mediated Knockin of Retinoschisin 1 Gene-A Potential Nonviral Therapeutic Solution for X-Linked Juvenile Retinoschisis.

The homology-independent targeted integration (HITI) strategy enables effective CRISPR/Cas9-mediated knockin of therapeutic genes in nondividing cells in vivo, promising general therapeutic solutions for treating genetic diseases like X-linked juvenile retinoschisis. Herein, supramolecular nanoparticle (SMNP) vectors are used for codelivery of two DNA plasmids-CRISPR-Cas9 genome-editing system and a therapeutic gene, Retinoschisin 1 (RS1)-enabling clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) knockin of the RS1 gene with HITI. Through small-scale combinatorial screenings, two SMNP vectors, with Cas9 and single guide RNA (sgRNA)-plasmid in one and Donor-RS1 and green fluorescent protein (GFP)-plasmid in the other, with optimal delivery performances are identified. These SMNP vectors are then employed for CRISPR/Cas9 knockin of RS1/GFP genes into the mouse Rosa26 safe-harbor site in vitro and in vivo. The in vivo study involves intravitreally injecting the two SMNP vectors into the mouse eyes, followed by repeated ocular imaging by fundus camera and optical coherence tomography, and pathological and molecular analyses of the harvested retina tissues. Mice ocular organs retain their anatomical integrity, a single-copy 3.0-kb RS1/GFP gene is precisely integrated into the Rosa26 site in the retinas, and the integrated RS1/GFP gene is expressed in the retinas, demonstrating CRISPR/Cas9 knockin of RS1/GFP gene

NtGNL1 Plays an Essential Role in Pollen Tube Tip Growth and Orientation Likely via Regulation of Post-Golgi Trafficking

Background: Tobacco GNOM LIKE 1 (NtGNL1), a new member of the Big/GBF family, is characterized by a sec 7 domain. Thus, we proposed that NtGNL1 may function in regulating pollen tube growth for vesicle trafficking. Methodology/Principal Findings: To test this hypothesis, we used an RNAi technique to down-regulate NtGNL1 expression and found that pollen tube growth and orientation were clearly inhibited. Cytological observations revealed that both timing and behavior of endocytosis was disrupted, and endosome trafficking to prevacuolar compartments (PVC) or multivesicular bodies (MVB) was altered in pollen tube tips. Moreover, NtGNL1 seemed to partially overlap with Golgi bodies, but clearly colocalized with putative late endosome compartments. We also observed that in such pollen tubes, the Golgi apparatus disassembled and fused with the endoplasmic reticulum, indicating abnormal post-Golgi trafficking. During this process, actin organization was also remodeled. Conclusions/Significance: Thus, we revealed that NtGNL1 is essential for pollen tube growth and orientation and it likel

Transcription Profile Analysis Reveals That Zygotic Division Results in Uneven Distribution of Specific Transcripts in Apical/Basal Cells of Tobacco

BACKGROUND: Asymmetric zygotic division in higher plants results in the formation of an apical cell and a basal cell. These two embryonic cells possess distinct morphologies and cell developmental fates. It has been proposed that unevenly distributed cell fate determinants and/or distinct cell transcript profiles may be the underlying reason for their distinct fates. However, neither of these hypotheses has convincing support due to technical limitations. METHODOLOGY/PRINCIPAL FINDINGS: Using laser-controlled microdissection, we isolated apical and basal cells and constructed cell type-specific cDNA libraries. Transcript profile analysis revealed difference in transcript composition. PCR and qPCR analysis confirmed that transcripts of selected embryogenesis-related genes were cell-type preferentially distributed. Some of the transcripts that existed in zygotes were found distinctly existed in apical or basal cells. The cell type specific de novo transcription was also found after zygotic cell division. CONCLUSIONS/SIGNIFICANCE: Thus, we found that the transcript diversity occurs between apical and basal cells. Asymmetric zygotic division results in the uneven distribution of some embryogenesis related transcripts in the two-celled proembryos, suggesting that a differential distribution of some specific transcripts in the apical or basal cells may involve in guiding the two cell types to different developmental destinies

Microanalysis Characterization and Immunomodulatory Effect for Selenium-Enriched Polysaccharide from <i>Morchella esculenta</i> (L.) Pers.

Morchella esculenta (L.) Pers., referred to as Morel, is a medicinal and edible homologous fungus, which contains many bioactive substances. In Morel, polysaccharides are the most abundant and have various bioactivities. In the present work, two novel polysaccharides, Se-MPS and MPS, were prepared and purified from selenium-enriched (Se-enriched) and common Morel mycelia, respectively, and their structural and immunomodulatory properties were evaluated. The results show that Se-enriched treatment significantly changed the polysaccharides’ chemical composition, molecular weight, and sugar chain configuration. In addition, the Se-enriched treatment also improved the polysaccharides’ fragmentation and thermal stability. Importantly, Se-enriched Morel polysaccharide (Se-MPS) could significantly enhance phagocytosis of RAW 264.7 macrophage cells and, remarkably, activate their immune response via activating the TLR4-TRAF6-MAPKs-NF-κB cascade signaling pathway, finally exerting an immunomodulatory function. Based on these findings, selenium-enriched Morel polysaccharide appears to have more potential for development and utilization in functional foods or medicines than ordinary Morel polysaccharide

Establishment of a Dual-Antigen Indirect ELISA Based on p30 and pB602L to Detect Antibodies against African Swine Fever Virus

African swine fever (ASF) is an acute, virulent, and highly fatal infectious disease caused by the African swine fever virus (ASFV). There is no effective vaccine or diagnostic method to prevent and control this disease currently, which highlights the significance of ASF early detection. In this study, we chose an early antigen and a late-expressed antigen to co-detect the target antibody, which not only helps in early detection but also improves accuracy and sensitivity. CP204L and B602L were successfully expressed as soluble proteins in an Escherichia coli vector system. By optimizing various conditions, a dual-antigen indirect ELISA for ASFV antibodies was established. The assay was non-cross-reactive with antibodies against the porcine reproductive and respiratory syndrome virus, classical swine fever virus, porcine circovirus type 2, and pseudorabies virus. The maximum serum dilution for detection of ASFV-positive sera was 1:1600. The intra-batch reproducibility coefficient of variation was <5% and the inter-batch reproducibility coefficient of variation was <10%. Compared with commercial kits, the dual-antigen indirect ELISA had good detection performance. In conclusion, we established a detection method with low cost, streamlined production process, and fewer instruments. It provides a new method for the serological diagnosis of ASF

Construction of a Near-Natural Estuarine Wetland Evaluation Index System Based on Analytical Hierarchy Process and Its Application

Nutrients carried in upstream rivers to lakes are the main cause of eutrophication. Building near-natural estuarine wetlands between rivers and lakes is an effective way to remove pollutants and restore the ecology of estuarine areas. However, for the existing estuarine wetland ecological restoration projects, there is a lack of corresponding evaluation methods and index systems to make a comprehensive assessment of their restoration effects. By summarizing a large amount of literature and doing field research, an index system was constructed by combining the characteristics of the near-natural estuarine wetlands themselves. It covered environmental benefits, technical management and maintenance, and socio-economic functions, and contained 3 systems, 7 criteria, and 16 indicators. The analytical hierarchy process (AHP) was used to determine the weights of each indicator. The top 5 indicators in order of importance were habitat diversity, total phosphorus (TP), coverage of aquatic plants, ammonia nitrogen (NH3-N), and adaptation to the surrounding landscape. The above evaluation system was used for the comprehensive evaluation of the water purification project in the Fuhe estuarine wetland, Hebei Province, as an example. The results showed that the comprehensive score of the Fuhe estuarine wetland at this stage was 4.1492, and the evaluation grade was excellent. The effect of water purification and ecological restoration was good, and the selected technology was suitable and stable in operation. It had a greater positive impact on the surrounding economy and society and can be promoted and applied. The research results were important for clarifying the advantages and defects of the project and developing efficient and advanced restoration technologies

Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus

African swine fever (ASF), the highly lethal swine infectious disease caused by the African swine fever virus (ASFV), is a great threat to the swine industry. There is no effective vaccine or diagnostic method to prevent and control this disease currently. The p30 protein of ASFV is an important target for serological diagnosis, expressed in the early stage of viral replication and has high immunogenicity and sequence conservatism. Here, the CP204L gene was cloned into the expression vector pET-30a (+), and the soluble p30 protein was successfully expressed in the E. coli prokaryotic expression system and then labeled with horseradish peroxidase (HRP) to be the enzyme-labeled antigen. Using the purified recombinant p30 protein, a double-antigen sandwich ELISA for ASFV antibody detection was developed. This method exhibits excellent specificity, sensitivity and reproducibility in clinical sample detection with lower cost and shorter production cycles. Taken together, this study provides technical support for antibody detection for ASFV

Virtual-Agent-Based Language Learning: A Scoping Review of Journal Publications from 2012 to 2022

Researchers have pointed out the importance of virtual agents in technology-supported language learning; however, how to effectively combine the two remains a challenge for educators and educational technologists. To this end, this study reviewed publications in the field of virtual-agent-based language learning research from 2012 to 2022 in the Web of Science SSCI Core Collection database and explored the dimensions of publication trends, country and regional distribution, participants, research methodology, research platforms, role of virtual agents, language proficiency, research hot topics, theoretical foundations, and hot issues and trends in the field of virtual-agent-based language learning research. Cluster and co-occurrence analysis using VOSviewer software was used to analyze the links among country and region distribution, keywords, and terms. It was found that (1) the top four regions in terms of the number of citations for authors were, in descending order, Iran, Japan, South Korea, and Brazil; (2) the learner characteristics that scholars were most concerned about were learning effectiveness, memory performance, social presence, learning experience, and motivation; and (3) the results of co-occurrence analyses classified virtual-agent-based language learning research into eight clusters, namely, anthropomorphic virtual agents, the effects produced by virtual agents, the social interaction of virtual agents, animated virtual agents and language achievement, the gestures of virtual agents, the effects of virtual agents on learner characteristics, computer-assisted learning, and the design of virtual agents. The lack of the systematic application of virtual agents in language learning prevented previous studies from revealing the language learning process in virtual-agent-based learning environments. Therefore, this study made appropriate recommendations for future investigations on how virtual agents can improve language learning for researchers, teachers, and decision makers