Virulence potential of Candida albicans isolated from oral cavity of patients with chronic renal failure on hemodialysis

Objective In patients with chronic renal failure (PCRF), the frequency of colonization of the oral cavity by yeasts of genus Candida spp. is high compared with healthy individuals. These yeasts have virulence factors that may contribute to the persistence of colonization and the development of these infections. The aim of this study was evaluate aspects of virulence from Candida albicans isolated from oral cavity of PCRF on dialysis. Methods This study was initially conducted with 49 clinical samples of C. albicans. The virulence factors assayed were produce of biofilm, germ tube, determination of adherence in oral epithelial cells and evaluation of resistance to the antimicrobial action of neutrophils and mononuclear cells. Results All isolates were highly efficient in forming biofilms on poly- styrene microplates, where 94% of the samples formed 4 + biofilm. Used as a screening test, of which three isolates were selected with different degrees of ability to form biofilm to assess other indicators of virulence. Overall, the isolates exhibited different characteristics regarding the virulence factors analyzed. It was also observed that the hypophosphorous acid (HOCl), production, one of leading inflammatory mediators with fungicidal action, also varied especially when the neutrophils, and not mononuclear cells, were stimulated with different samples. (Figure 1). Conclusion Therefore, our results indicate that C. albicans, is not only the most common species in the oral cavity of CRFP on dialysis, but also it presents the main virulence attributes, which reinforces the importance of monitoring of these patients towards the prevention of fungal infections

Retinal blood vessels extraction using probabilistic modelling

© 2014 Kaba et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.This article has been made available through the Brunel Open Access Publishing Fund.The analysis of retinal blood vessels plays an important role in detecting and treating retinal diseases. In this review, we present an automated method to segment blood vessels of fundus retinal image. The proposed method could be used to support a non-intrusive diagnosis in modern ophthalmology for early detection of retinal diseases, treatment evaluation or clinical study. This study combines the bias correction and an adaptive histogram equalisation to enhance the appearance of the blood vessels. Then the blood vessels are extracted using probabilistic modelling that is optimised by the expectation maximisation algorithm. The method is evaluated on fundus retinal images of STARE and DRIVE datasets. The experimental results are compared with some recently published methods of retinal blood vessels segmentation. The experimental results show that our method achieved the best overall performance and it is comparable to the performance of human experts.The Department of Information Systems, Computing and Mathematics, Brunel University

Gravitational Optics: Self-phase modulation and harmonic cascades

Nonlinear wave interaction of low amplitude gravitational waves in flat space-time is considered. Analogy with optics is established. It is shown that the flat metric space-time is equivalent to a centro-symmetric optical medium, with no second order susceptibility. The lowest order nonlinear effects are those due to the third order nonlinearity and include self-phase modulation and high harmonic generation. These processes lead to an efficient energy dilution of the gravitational wave energy over an increasingly large spectral range.Comment: 12 pages, REVTEX

Atmospheric super-rotation in solar system and extra-solar planetary atmospheres

Super-rotation is a common phenomenon in solar system planetary atmospheres. Out of the four substantial atmospheres possessed by solid bodies in the solar system, the slowly rotating planet, Venus, and moon, Titan, are both well-known to have atmospheres that rotate on average substantially more quickly than does the solid surface underneath. The more rapidly rotating planets, Mars and Earth, have much weaker global super-rotation, but both can exhibit time-varying prograde jets near the equator which rotate more rapidly than the local surface. Atmospheric super-rotation is not restricted to planets with solid surfaces and shallow atmospheres. Cloud-tracking observations of the gas giants Jupiter and Saturn show that they both possess rapid prograde equatorial jets and hence exhibit local super-rotation. Simplified global circulation models of extra-solar planets, including representations of ‘hot Jupiters’ and Earth-like planets rotating at different rates, can also show sustained super-rotating equatorial jets in different dynamical regimes. In the extra-solar planet cases in particular, the quantitative results are highly sensitive to model parameters. In each case the detailed mechanism, or combination of mechanisms, which produces the super-rotating jets might vary, but all require longitudinally asymmetric motions, waves or eddies, to transport angular momentum up-gradient into the jets. The mechanism is not always easy to diagnose from observations and requires careful modelling. We review both observations of solar system planets and recent global circulation model results, combined in the case of Mars and Earth in the form of atmospheric reanalyses by data assimilation, together with simplified extra-solar planet simulations

Avaliação qualitativa da água de barragens subterrâneas no semi-árido nordestino brasileiro.

Com o objetivo de avaliar a qualidade físico-química da água em áreas de captação/plantio de barragens subterrâneas, foram monitoradas oito barragens em propriedades localizadas nos Municípios de Petrolina-PE, Ouricuri-PE, Afrânio-PE e Filadélfia-BA, no período chuvoso de novembro 2006 a março de 2007. As análises fisicoquímicas permitiram avaliar a qualidade da água das barragens, empregando-se índices clássicos da literatura, como CE e RAS. Das oito barragens subterrâneas monitoradas, duas apresentaram problemas de salinidade, sendo que em uma delas a situação é mais grave devido, a presença de teores elevados de sódio. É fundamental, antes de construir uma barragem subterrânea, certificar-se da qualidade da água do riacho, bem como do solo circundante, através de análise destes dois recursos, assegurando, assim, maior eficiência da tecnologia.Edição dos Resumos do V Congresso Brasileiro de Agroecologia, Guarapari, 2007

Inverse Kinematics and Trajectory Planning Analysis of a Robotic Manipulator

In this work, we pretended to show and compare three methodologies used to solve the inverse kinematics of a 3 DOF robotic manipulator. The approaches are the algebraic method through Matlabreg; solve function, Genetic Algorithms (GAs), Artificial Neural Networks (ANNs). Another aspect considered is the trajectory planning of the manipulator, which allows the user to control the desired movement in the joint space. We compare polynomials of third, fourth and fifth orders for the solution of the chosen coordinates. The results show that the ANN method presented best results due to its configuration to show only feasible joint values, as also do the GA. In the trajectory planning the analysis lead to the fifth-order polynomial, which showed the smoothest solution

Cloned nucleoprotein (NP) gene of avian influenza virus for using as a positive control in syber green I real time RT-PCR (RRT-PCR).

Projeto/Plano de Ação: 11.11.11.111

Symptoms and patient factors associated with diagnostic intervals for pancreatic cancer (SYMPTOM pancreatic study): a prospective cohort study

This is the final version. Available on open access from Elsevier via the DOI in this recordBackground Pancreatic cancer is the tenth most common cancer in the UK; however, outcomes are poor, in part due to late diagnosis. We aimed to identify symptoms and other clinical and sociodemographic factors associated with pancreatic cancer diagnosis and diagnostic intervals. Methods We did this prospective cohort study at seven hospitals in two regions in England. We recruited participants aged 40 years or older who were referred for suspicion of pancreatic cancer. Data were collected by use of a patient questionnaire and primary care and hospital records. Descriptive and regression analyses were done to examine associations between symptoms and patient factors with the total diagnostic interval (time from onset of the first symptom to the date of diagnosis), comprising patient interval (time from first symptom to first presentation) and health system interval (time from first presentation to diagnosis). Findings We recruited 391 participants between Jan 1, 2011, and Dec 31, 2014 (24% response rate). 119 (30%) participants were diagnosed with pancreatic cancer (41 [34%] had metastatic disease), 47 (12%) with other cancers, and 225 (58%) with no cancer. 212 (54%) patients had multiple first symptoms whereas 161 (41%) patients had a solitary first symptom. In this referred population, no initial symptoms were reported more frequently by patients with cancer than by those with no cancer. Several subsequent symptoms predicted pancreatic cancer: jaundice (51 [49%] of 105 patients with pancreatic cancer vs 25 [12%] of 211 patients with no cancer; p<0·0001), fatigue (48/95 [51%] vs 40/155 [26%]; p=0·0001), change in bowel habit (36/87 [41%] vs 28/175 [16%]; p<0·0001), weight loss (55/100 [55%] vs 41/184 [22%]; p<0·0001), and decreased appetite (41/86 [48%] vs 41/156 [26%]; p=0·0011). There was no difference in any interval between patients with pancreatic cancer and those with no cancer (total diagnostic interval: median 117 days [IQR 57–234] vs 131 days [IQR 66–284]; p=0·32; patient interval 18 days [0–37] vs 15 days [1–62]; p=0·22; health system interval 76 days [28–161] vs 79 days [30–156]; p=0·68). Total diagnostic intervals were shorter when jaundice (hazard ratio [HR] 1·38, 95% CI 1·07–1·78; p=0·013) and decreased appetite (1·42, 1·11–1·82; p=0·0058) were reported as symptoms, and longer in patients presenting with indigestion (0·71, 0·56–0·89; p=0·0033), back pain (0·77, 0·59–0·99; p=0·040), diabetes (0·71, 0·52–0·97; p=0·029), and self-reported anxiety or depression, or both (0·67, 0·49–0·91; p=0·011). Health system intervals were likewise longer with indigestion (0·74, 0·58–0·95; p=0·0018), back pain (0·76, 0·58–0·99; p=0·044), diabetes (0·63, 0·45–0·89; p=0·0082), and self-reported anxiety or depression, or both (0·63, 0·46–0·88; p=0·0064), but were shorter with male sex (1·41, 1·1–1·81; p=0·0072) and decreased appetite (1·56, 1·19–2·06; p=0·0015). Weight loss was associated with longer patient intervals (HR 0·69, 95% CI 0·54–0·89; p=0·0047). Interpretation Although we identified no initial symptoms that differentiated people diagnosed with pancreatic cancer from those without pancreatic cancer, key additional symptoms might signal the disease. Health-care professionals should be vigilant to the possibility of pancreatic cancer in patients with evolving gastrointestinal and systemic symptoms, particularly in those with diabetes or mental health comorbidities. Funding National Institute for Health Research and Pancreatic Cancer Action.National Institute for Health Research (NIHR

Axions and their Relatives

A review of the status of axions and axion-like particles is given. Special attention is devoted to the recent results of the PVLAS collaboration, which are in conflict with the CAST data and with the astrophysical constraints. Solutions to the puzzle and the implications for new physics are discussed. The question of axion-like particles being dark matter is also addressed.Comment: Updated version of an invited talk at the Axion Training (CERN, December 2005). To appear as a Lecture Notes in Physics (Springer-Verlag), edited by B. Beltran, M. Kuster and G. Raffel