Anticancer properties of nanoparticle synthesized from Cyphostemma auriculatum Roxb. on nude mice

The present study was aimed to establish the pharmacological and therapeutic properties of a green synthesized silver nanoparticles (AgNPs) in breast cancer induced by 7,12-dimethylbenzanthracene (DMBA) in nude mice. In this study, AgNPs made from Cyphostemma auriculatum Roxb. leaf extract( CA-AgNPs) were tested in a nude mice model for anticancer activity. A significant elevate changes in blood chemistry like heamoglobin, RBC, WBC,  platelets and also on blood biochemical parameters such as catalase and SOD with obtained after 28 days of treatment with carcinogen. However, these levels were restored to normal at the end of the study period treated with CA-AgNPs. The liver oxidative stress enzymes showed no significant alterations. With 15 and 30 mg/kg b.w of CA-AgNP, histopathological analysis revealed no significant abnormalities in the kidney, spleen, lungs, heart, testis, or brain. However, 30 mg/kg b.w. of CA-AgNPs caused considerable cell edema and vacuolar degeneration in the liver, which returned to normal at the conclusion of the washout period. The findings of this study suggest that green produced CA-AgNPs at low concentrations could be beneficial

Anticancer Properties of Nanoparticle Synthesized From Cyphostemma Auriculatum Roxb. on Nude Mice

The present study was aimed to establish the pharmacological and therapeutic properties of a green synthesized silver nanoparticles (AgNPs) in breast cancer induced by 7,12-dimethylbenzanthracene (DMBA) in nude mice. In this study, AgNPs made from Cyphostemma auriculatum Roxb. leaf extract( CA-AgNPs) were tested in a nude mice model for anticancer activity. A significant elevate changes in blood chemistry like heamoglobin, RBC, WBC,  platelets and also on blood biochemical parameters such as catalase and SOD with obtained after 28 days of treatment with carcinogen. However, these levels were restored to normal at the end of the study period treated with CA-AgNPs. The liver oxidative stress enzymes showed no significant alterations. With 15 and 30 mg/kg b.w of CA-AgNP, histopathological analysis revealed no significant abnormalities in the kidney, spleen, lungs, heart, testis, or brain. However, 30 mg/kg b.w. of CA-AgNPs caused considerable cell edema and vacuolar degeneration in the liver, which returned to normal at the conclusion of the washout period. The findings of this study suggest that green produced CA-AgNPs at low concentrations could be beneficial

DataSheet1_Investigation of bioactive compounds from Bacillus sp. against protein homologs CDC42 of Colletotrichum gloeosporioides causing anthracnose disease in cassava by using molecular docking and dynamics studies.docx

Manihot esculenta, commonly called cassava, is an economically valuable crop and important staple food, grown in tropical and subtropical regions of the world. Demand for cassava in the food and fuel industry is growing worldwide. However, anthracnose disease caused by Colletotrichum gloeosporioides severely affects cassava yield and production. The bioactive molecules from Bacillus are widely used to control fungal diseases in several plants. Therefore, in this study, bioactive compounds (erucamide, behenic acid, palmitic acid, phenylacetic acid, and β-sitosterol) from Bacillus megaterium were assessed against CDC42, a key protein for virulence, from C. gloeosporioides. Structure of the CDC42 protein was generated through the comparative homology modeling method. The binding site of the ligands and the stability of the complex were analyzed through docking and molecular dynamics simulation studies, respectively. Furthermore, a protein interaction network was envisaged through the STRING database, followed by enrichment analysis in the WebGestalt tool. From the enrichment analysis, it is apparent that bioactive from B. megaterium chiefly targets the MAP kinase pathway that is essential for filamentous growth and virulence. Further exploration through experimental studies could be advantageous for cassava improvement as well as to combat against C. gloeosporioides pathogen.</p