Ozone Therapy in leg ulcers of patients with chronic venous insufficiency [abstract]

The term chronic venous insufficiency ? CVI (due to a deep vein thrombosis, valvular insufficiency or both) indicates a pathologic condition characterized by alterations of the venous flow and is the main cause in the formation of ulcers in the lower limbs, creating an important socio-medical problem, which finally leads the patient to a total disability for the rest of his/her life. In healthy leg veins, one-way valves allow blood to move only in one direction: upstream towards the heart. This prevents the backward flow of blood, back down the legs. However, in disease leg veins, the valves are enlarged and retracted, in such way, that they become unable to block the retrograde venous flow being the main cause for the appearance of ulcers in the lower limbs. Taking into account the different therapeutic effects of ozone as [1,2]: improvement of oxygen metabolism and blood rheologic properties, stimulation of the antioxidant defense system achieving the cell redox balance, modulation of the immunological system and nitric oxide, as well as its great germicide power, among others, the aim of this study was to evaluate the efficacy of ozone therapy on the healing process of chronic ulcers of the lower limb in comparison with conventional treatment. A controlled and randomized phase III clinical trial was performed in 90 hospitalized patients with ulcers (over 2 years of evolution, with a diameter of no less than 5 cm) of the lower limbs due to CVI [3]. Efficacy was evaluated according to the least time of hospital stay, which was the necessary time for the lesions to heal or when they were aseptic or their granulation tissue was ready for skin graft. In the cases where the patient did not want to be skin grafted, he/she was discharged and continued treatment at home with topic OLEOZON® (sunflower ozonated oil). The sample was divided at random into 3 groups of 30 patients each: 1-Control group (conventional treatment): venous repose (leg elevation), hyposodic diet, analgesics, daily cure with saline solution and fomentation with antiseptic solutions, several times per day. 2-Ozone group (M-AHT): venous repose (leg elevation), hyposodic diet, analgesics and local ozone treatment (bagging) and topical OLEOZON®, once per day, combining with M-AHT (3 times per week), up to 10 sessions (50 mg/L and 50 mL in 100 mL of blood). 3-Ozone group (Rectal): As M-AHT group but using rectal ozone application up to 20 sessions, once per day (50 mg/L and 200 L). No systemic or local antibiotics were used in the study. Results indicated an average length hospital stay of (53.1 ± 13.9) d for the control group and of (31.5 ± 6.7) d for both ozone group, with significant differences between control and ozone groups. Therefore, hospital stay was reduced for patients with ulcers in the lower limbs treated by ozone therapy, with the subsequent economic saving. No significant differences were observed between the two ozone groups in length hospital stay. No adverse reactions were seen. Ozone therapy can be considered as another treatment within the therapeutical supply for this condition, which could provide a fast rehabilitation for patients as well as their incorporation to a useful life

Importance of the toxicological tests in the application and safety of ozone therapy

Until today, there is not any official registry of the different routes of systemic ozone therapy. With the aim to prove the safety of this therapy, several ways of ozone (O3) applications were evaluated, such as rectal insufflation (RI), major autohemotherapy (M-AHT) and intraperitoneal application (IP). For RI, some toxicological tests were performed such as: acute, sub-chronic, mutagenic (bone marrow chromosomic aberrations and micronucleus), teratogenic  and irritation studies (at 40 mg/L and 10 mL during 15 days). For M-AHT, acute toxicological (in rats with 21, 47 and 64 mg/L and a volume of 2 mL with daily evaluation up to 15 days) and mutagenic (chromosomal aberration analysis, micronucleus assay and sister chromatid exchange test) studies were performed. For IP application, subchronic (O3 was administered during 15 days in mice at concentrations of 11, 29 and 35 mg/L and volumes of 80 mL/kg) and genotoxic (cytotoxic and clastogenic activity) studies were evaluated. All the toxicological studies performed for the O3 RI fulfilled the requirements established for an official registry (no deleterious effects were observed). However, more toxicological studies must be done with respect to M-AHT and IP O3 applications in order to prove the complete safety of these ways

Medical ozone on hamstring injury in a professional athlete assessed by thermography: a clinical case report

Injuries associated with the hamstring muscles in the running athlete are increasingly investigated due to the economic and functional consequences associated with them. Although hardly used in the treatment of sports injuries, medical ozone is effective and very well tolerated in the treatment of musculoskeletal pain, it was decided to add a series of medical ozone infiltrations to the treatment. The evolution of the case was recorded by medical thermography, in addition to measuring pain intensity (visual analog scale) and functional capacity (toe touch test). Pain intensity (visual analog scale) decreased from seven at baseline to two at the end of treatment (after two ozone infiltrations, one weekly). Mobility of the damaged area (toe touch test) improved from a distance of 8 cm at baseline to 0 cm at the end of treatment. Regarding medical thermography, after the first and second infiltration of ozone, the temperature rose to a significant increase in perfusion from baseline from 31.2 to 31.8 & DEG;C and from 31.2 to 32 & DEG;C, respectively. These results suggest the possible interest of medical ozone as an adjuvant treatment for the recovery of sports tendinopathies and encourage us to carry out further studies

Ozone Therapy in diabetes and its complications [abstract]

In Diabetes mellitus, long-term complications, that cause morbidity and premature mortality, are characterized by microvascular and macrovascular diseases. A more frequent concomitant of distal anesthesia is the development of neurotropic ulceration, particularly on the plantar aspect of the foot. All these events characterize the underlying mechanisms that may lead to rapid gangrene after foot injury. Vascular endothelium appears to be a vulnerable target for hyperglycemia-induced metabolic changes. Activation of polyol pathway, non-enzymatic glycosilation of proteins and the increase of reactive oxygen species (ROS) play an important role in diabetes complications. Ozone has been used as a therapeutical agent and beneficial effects have been observed. However, so far only a few biochemical and pharmacodynamic mechanisms have been elucidated. We demonstrated that controlled ozone administration may promote an oxidative preconditioning or adaptation to oxidative stress, preventing the damage induced by ROS [1]. Taking into account that diabetes is a disorder associated with oxidative stress, we postulate that ozone treatment might protect antioxidant systems and maintain, at a physiological level, other markers of endothelial cell damage associated with diabetic complications. In this study we evaluate ozone efficacy in a preclinical diabetes animal model [2]and in a clinical trial with type 2 diabetes patients suffering of diabetic foot complications [3]. Diabetes animal model [2]: Five groups of rats were classified as follows: (1) control group treated only with physiological saline solution; (2) positive control group using streptozotocin (STZ) as a diabetes inductor; (3) ozone group, receiving 10 treatments (1.1 mg / kg), one per day after STZ-induced diabetes; (4) oxygen group (26 mg /kg), one per day, as in group 3, but using oxygen only and (5) control ozone group, as in group 3, but without STZ. The following parameters were evaluated: plasma glucose concentration; in pancreas homogenates: levels of aldose reductase, fructolysine, advanced oxidation protein products, nitrite/nitrates (as an index of nitric oxide), glutathione (GSH), total hydroperoxides (TH), malondialdehide concentration (MDA), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities as indicators of redox balance. Pancreas morphology was evaluated by light microscopy. Clinical assay [3]: Randomized controlled clinical trial where all patients provided a signed informed consent before being enrolled was performed. Adult hospitalized patients of both sex of any ethnic, with diagnosis of neuroinfectious diabetes foot suffering of ulcers of the feet and lower extremities were eligible to participate in the study. These patients must not meet any of the following criteria: severe septic conditions, hypersensibility to the medication that will be used, hepatic dysfunction, renal failure (serum creatinine level > 1.32 mol/L), pregnancy, cancer, current therapy with any immunosuppresive agent or anticonvulsant. Patients were randomized to two different groups of treatment: 1- control, 50 patients treated with antibiotic therapy, systemic and topically in the lesion (during 20 days), and 2- ozone, 52 patients treated daily with ozone, 20 sessions, by rectal insufflation (with an ozone dose of 10 mg, ozone concentration: 40 mg/L) and locally. The same biochemical parameters that were taking into account in the preclinical study were measured in plasma, at the beginning and at the end of the treatments, as well as the clinical evaluation of the lesions and length of hospitalization. In both studies, ozone treatment improved glycemic control, and prevented oxidative stress, the increase of fructolysine content and advanced oxidation protein products. However, high plasma glucose figures and oxidative stress were maintained in the antibiotic group. In the animal model, ozone treatment also improved pancreas integrity. In the clinical assay, at the end of the treatments, a decrease of the area and perimeter of the lesions, for both groups, was obtained, but the expected total recovery showed that patients treated with ozone needed half of the time to achieve it, with regard to the antibiotic group. No significant differences between both treatments were obtained for the variable clinical evaluation (qualitative clinical evaluation), but with a trend to increase the number of cured patients and to decrease the non-cured patients in the ozone group, in comparison with also decreased the area to be amputated. The length of hospitalization decreased in patients treated with ozone with regard to the antibiotic group. Also, a controlled, randomized and double blind clinical trial was performed in 21 patients with a diagnostic criterion of peripheral diabetic neuropathy (DN) which were treated with ozone (ozone group) or with oxygen (oxygen group), both by rectal insufflation [4]. The results of this study showed that repeated administration of ozone in non-toxic doses played a role in the control of diabetes and its complications. It is important to emphasize the ozone treatment effect in the increase of SOD, as well as to consider it a molecular target in this syndrome. This is an interesting explanation, since it has been recognized anion superoxide radical as the link of four pathogenic pathways associated to micro and macro vascular complications in diabetes. The ozone treatment, in patients with diabetes type 2 suffering of neuroinfectious diabetic foot, improved glycemic control and prevented oxidative stress associated to diabetes mellitus and its complications, maintaining a cellular redox balance, in agreement with the excellent results obtained clinically in these patients. Patients with DN treated with ozone showed significant statistical increases in amplitude of peroneal (proximal and distal) nerve for both legs and nerve conduction velocity, as well as improvement in numbness, paresthesia and painful dysesthesia of the feet. However, no significant modification was observed in patients treated with oxygen in any of the electrophysiological indicators or clinical symptoms measured, between the pre and post treatment values No side effects were observed. Ozone therapy could be a future alternative in the therapy of diabetes and its complications

Clinical behavior of children with infantile cerebral palsy after ozone therapy

Objective. The aim of this study was to determine the usefulness of ozone therapy in the treatment of Infantile Cerebral Palsy (ICP). Patients and methods. A non-controlled clinical assay was made in the Ozone Research Center (CIO), Havana, Cuba from January 2013 to January 2014. The sample was constituted by patients remitted to pediatrics consultation of CIO, to whom inclusion and exclusion criteria were applied. The study group involved 45 patients, from 1 month of birth to 8 years, with cerebral palsy of hypoxic-ischemic cause. The evaluation criteria were: evolution of the motor disorder according to the Gross Motor Function Classification System (GMFCS) scale, modification of muscle tone (Ashworth modified scale) and response to treatment (O?Brien modified scale). The way of administration was rectal insufflation; concentrations between 15, 20, 25 and 30 mg/L were used, volumes varied according to age, making calculation of the dose of ozone according to kilograms of weight. Cycles of 20 sessions, every 3 months were indicated, until completing 4 in 16 months. Patients were clinically evaluated, according to the scales used, before and after each cycle. Results and Discussion. The best answer to treatment was obtained in the group aged ? 4 years. The variables analyzed showed a significant improvement when the ozone treatment concluded. With respect to the evolution of the motor disorder, in 65 % of cases it improved. In the group of children below 4 years, the response was better in relation to the muscle tone. Response to treatment, according to the relatives? criteria, was of 70 % of the children with marked improvement in the tone and muscle function. Conclusions. The greatest percentage of patients improved in the evolution of the motor disorder; when the Manual Ability Classification System (MACS) scale was applied, more than half the patients showed an improvement. A high percentage of children get a satisfactory result regarding muscle tone and motor function. No side effects were present in any of the cases during the study

PAPEL NEFROPROTECTOR DEL OZONO EN EL DAÑO RENAL CRÓNICO EXPERIMENTAL

Los productos avanzados de la glicosilación de proteínas (PAGP) constituyen mediadores importantes que ocasionan daño al tejido renal en diferentes situaciones clínicas. Conociendo los efectos nefroprotectores de la ozonoterapia rectal (OTR), el objetivo del estudio fue demostrar la influencia de esta terapia en los niveles de fructosamina como PAGP y la actividad de la fosfolipasa A2 en tejido renal con insuficiencia renal crónica por ablación de la masa renal (AMR) en ratas. Se seleccionaron ratas Wistar hembra con peso corporal entre 200 y 220 g, se dividieron en cuatro grupos experimentales: un grupo control sin daño renal, otro grupo control con daño renal por AMR y otros dos grupos también con daño renal y tratados con ozonoterapia y oxígeno también por vía rectal, respectivamente. Los resultados evidenciaron la reducción significativa p< 0,05 de los niveles de fructosamina y la actividad de la fosfolipasa A2 en el tejido renal de ratas tratadas con OTR.  Por lo que se concluye que la OTR demostró acción antinflamatoria, como mecanismo contribuyente a la preservación de la estructura y función del riñón sometido a daño renal experimental crónico, por ablación de la masa rena

Ozone Therapy : Scientific updates and researches in recent years [abstract]

Ozone medical applications gather strength, day by day. Ozone, in its oxidant status, administered in low doses and in a number of controlled treatments, is able to trigger the endogenous antioxidant systems achieving a redox balance. As such, it is able to control chronic oxidative stress associated with many diseases. Moreover, levels of nitric oxide and pro-inflammatory cytokines, like tumor necrosis factor (TNF-alfa) and interleukin 1 and 6, were able to be modulated by ozone. Also, mitochondrial integrity and functionality was preserved, calcium homeostasis was achieved, adenosine A1 receptors were activated and activity of the superoxide dismutase enzyme was regulated [1]. In this lecture, preclinical and clinical studies of recent researches developed in Cuba are presented. Two topics have been selected: Alcoholism [2,3] and arthritis [4,5]. In today's world, alcoholism is a serious health and social problem, affecting millions of people, without distinction of race, sex, culture or latitude. One diagnostic criterion of alcohol dependence is the appearance of a withdrawal syndrome when alcohol consumption ceases. Experimental studies have demonstrated that many consequences of withdrawal found in animals resemble those observed in humans. Such signs and symptoms of ethanol withdrawal (EW) include enhanced activity of the autonomic nervous system; body posture and motor abnormalities; hyperexcitability of the central nervous system (CNS), including sensory hyperreactivity, convulsion, anxiety, etc. Chronic ingestion of high levels of alcohol may bring about oxidative stress, associated with hepatic alterations, and of CNS, mainly due to the formation, through alcohol metabolism, of free radicals, acetaldehyde, lipid and protein oxidation and their reaction products. The protective effects of ozone on brain injury induced by oxidative stress and behavioral changes in rats, after 2 weeks of EW, were studied [2]. Also, a clinical trial with 10 alcoholic patients (elapsed time after ethanol withdrawal: less or equal than 3 weeks) treated with ozone by rectal insufflation (ozone concentration from 20 to 35 mg/L and volumes from 100 to 150 ml) was performed [3]. In the preclinical study [2], 4 groups of rats (n=10 each one) were settled: I-Control, II-Ethanol, III- Ethanol + Ozone (20 mg/L, dose of 1 mg/kg, 10 sessions by rectal insufflation) and IV- Ethanol + Oxygen. At the end of EW, rats were subjected to behavioral tests followed by brain tissue collection to measure markers of oxidative damage. Ozone increased food consumption, maintained water intake at the same levels as the control group and reestablished cellular redox status. Anxiety, locomotor activity and memory/learning of the rats were improved. Ozone protected the brain against oxidative injury, improving important functions of the CNS. In the clinical study [3], the results demonstrated that ozone improved 70% of the signs from Clinical Institute Withdrawal Scale (CIWA-Ar), mainly those associated to CNS. Ozone efficacy was observed in patients that required pharmacological treatment. Reduction of CIWA-Ar scores and the oxidative stress (

Ozone Therapy : Teratogenic study of ozone. Possible indications in obstetrics and gynecology [abstract]

Ozone is a powerful oxidant, surpassed, in this regard, only by fluorine. Its doubtless strong reactivity has contributed to establish the dogma that ozone is always toxic and its medical application must be proscribed. However, ozone therapy has been used for therapeutic purposes since the beginning of the last century and its use is increasingly demanded nowadays [1]. For proper enlightenment and guidance of the person interested in the use of ozone for medical purposes, scientific documentation on the safety/toxicity profile of this acclaimed procedure becomes necessary. Taking into account that the National Health Regulatory Agencies require of documentation with scientific trials for sanitary registration of drugs and therapy procedures, different toxicological tests were performed in Cuba using experimental animals, following the guidelines from the Cuban Regulatory Agency, Food Drug Administration, International Standards Organization and the Health World Organization aimed at proving the safety of ozone therapy administration. In this lecture, the teratogenic study of ozone by rectal insufflation (RI) is presented [2]. Female Wistar rats (200-250 g) were divided into 3 experimental groups: 1- control (n=15), without any treatment; 2- treated with 1 mL of ozone (n=17) at a concentration of 34 µg/mL (150 µg/kg); 3- treated with 4 mL of ozone (n=17) at a concentration of 90 µg/mL (1600 µg/kg). Ozone was administered by RI during 10 sessions, from the 6th to the 15th day of gestation. The pregnancy was confirmed by the presence of spermatozoa in the vaginal exudates. It was considered that the animals with positive exudates were in the 0 day of gestation. In the 19th day of gestation, animals were euthanized by an ether overdose and the fetuses were obtained by cesarean. The number of corpus luteum, implantations, alive and dead fetuses, reabsorptions, as well as the weight and the length cranium-caudal of each fetus were registered. Alive fetuses were examined to find any external malformations. No toxic effect was observed in the pregnant rats subjected to the ozone treatment by RI. Mother weight gain did not show significant differences among the groups, neither the other indicators (number of corpus luteum, implantations, alive and dead fetus, reabsorptions). In respect to the fetus morphology, no external, skeletal or visceral malformations were observed. The weight and the length cranium-causal did not show significant differences among the groups. It is concluded that no teratogenic nor embriotoxic effects were found after the ozone application by rectal insufflation at the doses studied. With respect to the application of ozone therapy in Obstetrics, an update of the papers published about this topic was presented [3-6]. They referred the use of ozone therapy in: pregnant womens with hypertension, genital or no genital infections during pregnancy, threatened abortion, intrauterine fetal hypoxia, among others. For example, the effect of ozone therapy by rectal insufflation on fetoplacental blood flow in hypertensive pregnant women, with 24 weeks of gestation, indicated a better fetoplacental blood flow, achieving a greater oxygenation in the group treated with ozone plus antihypertensive treatment (methyldopa) in comparison with a control group treated only with the conventional treatment. Also, a reduction of methyldopa in 23.7 % of the initial doses was found in the ozone group as opposed to an increase of 40.8% in the control group. Another study about ozone therapy as the main component of the complex treatment of threatened abortion, using ozonated saline solution in the first (group I) or second semester (group II) showed a decrease in the molecular products of lipid peroxidation and a simultaneous increase in the antioxidant activity. Also, with ozone therapy the pregnancy was preserved and prolonged to the physiological terms of labor in 86% of patients inside group I and in 85% of patients inside group II. However, in the control group (conventional treatment) I and II, it was possible to preserve the pregnancy in 65 and 60% of cases, respectively. In the ozone group, 80% of newborn babies received 7-10 points (maximum value) according to Apgar's score, however, only 58.3% in the control group. In Gynecology, an interesting paper was reviewed about distal fallopian tube recanalization using ozone treatment. In the ozone group, the ozone was introduced in the uterine cavity and in the case of the control group, anti-inflammatory drugs and antibiotics was used. The overall recanalization rate was significantly higher in the ozone group (93%) as compared to the control group (79%) 6-months after intervention (p < 0.01). The re-adhesion rate in the ozone group was significantly lower than the control group (p < 0.01). The pregnancy rate after 12 months of intervention was significantly higher in the ozone group (59%) compared to the control group (43%) (p < 0.05). These results were highly encouraging and hold promise for treating distal tubal obstruction in infertile females. For inflammatory diseases in female genital organs, intravaginal ozone has been used. Ozone restores the body's own defense abilities by the normalization of vaginal mucosa local immunity, prevents the generalization of inflammatory processes, facilitates uterus healing, reduces the treatment time with no complications or side effects. In Cuba we have a vast experience in the use of ozonated sunflower oil in the treatment of vulvovaginitis, human papillomavirus, acuminate condyloma, genitalis herpes virus with very good results [7-9]. In summary, we can state that preclinical and clinical studies presented here about the application of ozone therapy in Obstetrics and Gynecology, in the different ways of ozone applications, do not demonstrate any adverse effect or complication. However, more controlled clinical trials are necessary to perform in order to prove the inocuity of ozone therapy, mainly in Obstetrics, where threre are still questions to be clarified

Ozone Therapy : General protocols based on evidences [abstract]

Ozone has been used as a therapeutical agent for the treatment of different diseases and beneficial effects have been observed. However, ozone biological effects remain controversial due to the scarce knowledge of its biochemical and pharmacodynamic mechanisms [1]. Taking into account that ozone therapy is gathering more interest, day by day, and it has been used in apparently nonrelated diseases with beneficial effects, the National Center for Scientific Research (Havana, Cuba) in collaboration with different research centers and health institutions have studied the ozone toxicology and its pharmacological actions as well as its biochemical mechanisms. The most remarkable experiences, in preclinical and clinical studies, developed in Cuba during the last 30 years, in order to prove unequivocally ozone therapy validity, are presented in this lecture. On the basis of the oxidant properties of ozone, we postulate that controlled ozone administration (named as ozone oxidative pre-/postconditioning mechanism) is able to promote a slight and transient oxidative stress which in turn re-establishes the signalling pathways which have been lost in pathological conditions, preserving the cellular redox balance (increasing antioxidant endogenous system), mitochondrial function as well as the regulation of transcription factors and the modulation of the immunological system [2]. It is evident that membrane-associated ozone peroxides, 4-hydroxialkenals, superoxide anion, nitric oxide, among others, are going to play an important role in cellular signals as well as in the pathology of different diseases [2,3]. Regulation of these biomolecules by ozone preconditioning has been demonstrated in several preclinical and clinical studies as ischaemia-reperfusion phenomenom, parkinson, senile dementia, disk herniation, retinitis pigmentosa, ischemic cardiopathy, arterial insufficiencies, diabetes, osteoarthritis, asthma, vestibulocochlear syndrome, among others [2,4]. Nitric oxide modulation, as well as the increase in A1 adenosine receptors achieved with this therapy has an important role in brain blood flux, in the formation of memory, in the release of neurotransmitters and in the inflammatory processes [5,6]. Ozone therapy seems to induce a simultaneous resuscitation of functions that had gone wrong, reactivating and re-equilibrating physiological activities. This lecture contributes to clarify the ozone mechanism of action and its different pharmacology effects. It is concluded that ozone therapy can be useful in the treatment of several diseases, either as adjuvant of ortodox medicine or taking part positively in those where conventional therapy has had no success, all this with a common purpose: to favour the patients and contribute to a better quality of life

Efectos del aceite ozonizado en la conjuntivitis hemorrágica epidémica

Epidemic Hemorrhagic Conjunctivitis (EHC) is a self-limited, conjunctiva inflammation of viral etiology which affe cts all a ges a nd ta kes place in epidemic form. Its main symptoms are sensati on of fore ign bod i es, lacrimation, p hotose nsitivity, general discomfort a nd pain. Its c ritical sign s are subconjunctival hemorrhage s, follicular reaction and pre-auricular adenopathy. Also, serous secretion, chemosis, superficial punctate keratitis and palpebral ptosis are observed. Taking into ac co unt the broad spectrum germicide power of OLEOZON (ozonized sunflower oil), as well as its degree of anti-inflammatory character, the aim of this study was to evaluate the effectiveness of this medication in i ts collyrium f orm for t he treatment of EHC. In ¨Dr. Salvador Allende¨ Clinical Hospital, 20 patients were treated with EHC in October, 2009. Twelve of them received treatment with OLEOZON collyrium (one drop twice per day) and 8, making up the control group, received conventional treatment (cold compresses, non ste roidal antiinflammatory dr ugs, yodoxuridine in collyrium o r recom binant alfa-2b interferon). All p atie nts treate d with OL EOZON unde rwe nt a fast evolution toward recovery. In 72 h, they showed signs of great improvement and in 1 week they were totally cured. No patients presented complications. In the control group the evolution was more prolonged, mainly in patients show ing complications (3 with keratitis ). Treatment of EHC with OLEOZON collyrium provides with very positive results in this disease.La Conjuntivitis Hemorrágica Epidémica (CHE) es una inflamación conjuntival de etiología viral, autolimitada que afecta a todas las edades y cursa de forma epidémica. Su s sí ntomas fundamenta l es son sensació n de cuer po extr año, lagrimeo , foto sensibil i dad, malestar gen eral y dolor. Sus signos crí ticos son hemorragias subconjuntivales, reacción folicular y adenopatías pre auriculares. También se observa secrec i ón serosa, quémosis, querati tis superficial p unteada y pto sis palpebra l . Teniendo en cuenta el poder germicida de amplio espectro del OLEOZON (aceite de girasol ozonizad o), as í como su cierto carácter ant iinf lamatorio, el o bjetivo de e ste trabajo fue evaluar la efectividad de este medicamento en su forma de colirio para el tratamiento de la CHE. En el Hospital Docente ¨Dr Salvador Allende¨ fueron atendidos 20 pacientes con CHE en Octubre del 2009. De ellos 12 recibieron tratamiento con OLEOZON colirio (1 gota dos veces al día) y 8 conformaron el grupo control los cuales recibieron tratamiento convencional (fomentos fríos, antinflamatorios no esteroideos, yodoxuridina en colirio ó interferón alfa-2b recombinante). Todos los pacientes tratados con OLEOZON tuvieron una rápida evolución hacia la curación. A las 72 h mostraban signos de gran mejoría y a la semana estaban totalmente curados. Ninguno de los pacien tes presentó comp l i caciones. En el grupo control la evolución fue más prolongada, fundamentalmente en los pacientes que presentaron complicaciones (3 con queratitis). El tratamiento de la CHE con OLEOZON colirio brinda resultados muy positivos en esta patologí