Formulating O/W Emulsions with Plant-Based Actives: A Stability Challenge for an Effective Product

Quality, safety, and efficacy concerns added to instability, poor absorption, and the dispersion of actives are common problems while formulating plant-based cosmetics. Furthermore, a correct balance between the stability of the emulsion, the sensory profile, and the high efficacy has to be considered to formulate an effective product. In this paper, we demonstrate that rheology is a methodological tool that can be used while designing a new product. In particular, we developed an O/W emulsion which is easy to spread on irritated skin, and that can soothe the redness and discomfort caused by the exposure to both physical and chemical irritating agents. The green active mixture consists of three natural raw materials: Bosexil\uae, Zanthalene\uae, and Xilogel\uae. Each ingredient has a well-demonstrated efficacy in terms of soothing, anti-itching, and moisturizing properties respectively. Starting from the selection of a new green emulsifying system, through the analysis of the rheological properties, we obtained a stable and easy-to-apply o/w emulsion. The efficacy of the optimized product was assessed in vitro on intact and injured skin using the SkinEthic\u2122 Reconstituted Human Epidermis (RHE) as a biological model

Bacterial flora associated with the digestive tract of gilthead seabreams reared in floating cages in the Alghero Bay (North western Sardinia, Italy): preliminary results

The intestinal microflora of fish is highly variable and can depend on many factors such as species, developmental stage, environmental conditions, trophic habits and, in the case of farmed fish, on rearing conditions (i.e., fish density, quality of rearing water, dietary regimen, etc.). Different studies showed that Gram-negative bacteria such as Enterobacteriaceae and the Vibrio-Aeromonas group dominate the fish intestine. However, since few studies have been carried out on the bacterial flora of intensively reared marine teleosts, the aim of the present study was to investigate the microflora associated with the digestive tract of Sparus aurata specimens reared in floating cages. A total of 30 gilthead seabreams (mean weight 324.9±61.4 g) starved for 48 hours was sampled in autumn 2008 from a fish farming facility located in the Alghero Bay (North western Sardinia, Italy: Lat 40°33’43.9’’N, Long 8°16’09.0’’E). The intestine between the pyloric caeca and the anus of each specimen was removed and analysed by means of conventional bacteriological techniques using five culture media: Plate Count Agar (PCA), Nutrient Agar (NA), Violet Red Bile Glucose Agar (VRBGA), Violet Red Bile Agar Mug (VRBA-MUG), and de Man-Rogosa-Sharpe (MRS) agar. Total viable counts on PCA and NA were quite similar, showing mean values of 126.7±109.0 colony forming units (cfu) per gram intestinal tissue (between 10.0 and 495.0 cfu/g) and 108.0±101.6 cfu/g (from nil to 350.0 cfu/g), respectively. The mean number of Enterobacteriaceae determined on VRBGA proved to be 46.3±42.8 cfu/g (from nil to 150 cfu/ g) and the VRBA-MUG mean count were 30.4±29.3 cfu/g (from nil to 95.0 cfu/g). No bacterial colonies were instead detected using the MRS medium. These results, although preliminary, evidenced a relative low number of bacteria associated with the digestive tract of the fish examined, thus indicating good hygienic conditions inside the cages as well as a suitable rearing density and a balanced diet for the seabreams

Fair Voice Biometrics: Impact of Demographic Imbalance on Group Fairness in Speaker Recognition

Speaker recognition systems are playing a key role in modern online applications. Though the susceptibility of these systems to discrimination according to group fairness metrics has been recently studied, their assessment has been mainly focused on the difference in equal error rate across groups, not accounting for other fairness criteria important in anti-discrimination policies, defined for demographic groups characterized by sensitive attributes. In this paper, we therefore study how existing group fairness metrics relate with the balancing settings of the training data set in speaker recognition. We conduct this analysis by operationalizing several definitions of fairness and monitoring them under varied data balancing settings. Experiments performed on three deep neural architectures, evaluated on a data set including gender/age-based groups, show that balancing group representation positively impacts on fairness and that the friction across security, usability, and fairness depends on the fairness metric and the recognition threshold

A purely bioinformatic pipeline for the prediction of mammalian odorant receptor gene enhancers

Background In most mammals, a vast array of genes coding for chemosensory receptors mediates olfaction. Odorant receptor (OR) genes generally constitute the largest multifamily (> 1100 intact members in the mouse). From the whole pool, each olfactory neuron expresses a single OR allele following poorly characterized mechanisms termed OR gene choice. OR genes are found in genomic aggregations known as clusters. Nearby enhancers, named elements, are crucial regulators of OR gene choice. Despite their importance, searching for new elements is burdensome. Other chemosensory receptor genes responsible for smell adhere to expression modalities resembling OR gene choice, and are arranged in genomic clusters - often with chromosomal linkage to OR genes. Still, no elements are known for them. Results Here we present an inexpensive framework aimed at predicting elements. We redefine cluster identity by focusing on multiple receptor gene families at once, and exemplify thirty - not necessarily OR-exclusive - novel candidate enhancers. Conclusions The pipeline we introduce could guide future in vivo work aimed at discovering/validating new elements. In addition, our study provides an updated and comprehensive classification of all genomic loci responsible for the transduction of olfactory signals in mammals

Suspended culture of Ostrea edulis in the Calich lagoon (North western Sardinia, Italy): preliminary results

Suspended culture is a widespread farming method used for many bivalve species such as mussels, oysters and scallops. In the Mediterranean, this technique is mainly practised in lagoons or in sheltered coastal areas using floating lines from which molluscs are suspended in several ways. In this study, the European flat oyster (Ostrea edulis Linné, 1758) was grown in suspended lantern nets in the Calich lagoon (Sardinia, Mediterranean Sea) from March 2004 to March 2005. Two distinct groups of 6 lanterns each were hung to longline ropes near the mouth (station 1) and in the central portion of the lagoon (station 2). In each lantern (diameter=50cm; height=30cm), 90 O. edulis specimens were grown and, in order to ensure good water circulation inside the lantern net, fouling organisms were removed every month. Overall mortality, shell length (anterior-posterior axis), shell width (maximum distance on the lateral axis, between both valves of the closed shell) and total wet weight of a 180 specimen oyster sample (30 from each lantern) were recorded every 2 months at each site. In addition, water temperature, salinity, dissolved oxygen, and pH were monitored monthly by means of a multi-parametric probe at both sites (between 10a.m. and 12p.m.). One-way ANOVA was used to test for differences in oyster final mean morphometric characters recorded at the 2 growing stations. Chi-square test (with Yates correction for continuity) was performed to compare survival rates at the end of the trial. From an initial mean shell length of 49.5±4.6mm, O. edulis growth rate showed a similar trend at both the stations. Nevertheless, ANOVA detected significant differences (F=7.10; p<0.01) in final mean oyster length values (83.7±6.5mm at station 1 vs 81.7±7.6mm at station 2). Significant differences (F=9.74; p<0.01) were also found in final mean oyster width (28.4±3.1mm at station 1 vs 27.5±2.8mm at station 2) and weight (F=4.00; p<0.05) values (91.4±16.7g at station 1 vs 87.7±18.3g at station 2). Moreover, chi-square test revealed a significantly different survival rate (χ2=10.04; p<0.01) between the 2 groups (57.4% at station 1 vs 47.6% at station 2).Water temperature, salinity, dissolved oxygen, and pH monthly values recorded at the 2 growing stations were almost identical. Thus, the observed differences in oyster growth and survival seemed not to be due to the hydrological variables considered. Instead, they could probably be related to mechanical and chemical effects of water renewal by coastal waters, which may have led to different seasonal seston food supplies at the 2 sites. The suspended culture of the European flat oyster described in this paper can increase the mollusc production of the Calich lagoon by growing a valuable bivalve species which is naturally scarce in this biotope. In fact, our preliminary results showed good survival and growth rates of O. edulis especially near the mouth of the lagoon. Furthermore, this farming technique could be a possible source of economic benefits for local fishermen and, above all, a low impact aquacultural activity compatible with the environment

Prime valutazioni degli effetti della protezione sull’ittiofauna di fondo roccioso nella costa occidentale dell’Isola dell’Asinara = Preliminary evaluation of the effects of protection on rocky fish assemblages in the Western coast of Asinara Island

Coastal fish fauna from a recently established Mediterranean MPA and from an adjacent nonprotected zone near its boundary were compared to evaluate the effects of protection. A remarkable “reserve effect” was detected for the whole assemblage in terms of both species richness and abundance. Moreover, a moderate spillover effect outside the MPA was observed for several target species

Response of captive seabass and seabream as behavioural indicator in aquaculture

Welfare of cultivate fish at high-density represents an important concern for modern aquaculture. The behaviour of European seabass (Dicentrarchus labrax) and seabream (Sparus aurata) reared in cages was studied in a fish farm of northern Sardinia (Italy) in autumn 2006 to test whether captive condition had an effect on the movement patterns of these two species.Video images recorded before, during and after the manual feeding distribution allowed us to collect data on different behaviours of captive fish. Thus, behaviours indicating the position of fish in the water column, swimming direction and possible aggressive behaviours (aggression, direction change and collision) showed juveniles and adults of seabass and seabream were overall affected by feeding rhythms and captive overcrowding. Seabream had a major tendency to swim towards the bottom and higher frequency of horizontal swimming and collisions than seabass. The overall behavioural difference between two species was explained in terms of their differences in ecological features in the wild

Variazioni spazio-temporali della fauna ittica nelle pozze di scogliera del litorale di Budoni (Sardegna Nord Orientale) = Spatio-temporal variations of ichthyofauna in the tidal rockpools from the Coast of Budoni (North Eastern Sardinia)

Fish fauna of tidal pools was investigated in a coastal district of Sardinia (central western Mediterranean) between October 2007 and October 2008. Overall, 206 specimens from 7 species belonging to 5 families were recorded in two groups of tidal pools with a different coastal exposure. Significant differences in the number of individuals were detected for tidal pools differently exposed, while number of species, number of individuals, and biomass were significantly different among the sampling periods

Morphometric relationships and annual gonad index of the edible Sea urchin <i>Paracentrotus lividus</i> from North Western Sardinia = Relazioni morfometriche e indice gonadico annuale del riccio di mare commestibile <i>Paracentrotus lividus</i> nel nord ovest Sardegna

Weight/diameter, weight/height and height/diameter morphometric relationships were calculated for a shallow rocky Paracentrotus lividus population sampled monthly near Alghero (Italy) from November 2004 to October 2005. Gonad index (GI) of the sea urchins was also evaluated. Statistical analyses showed significant differences of GI between 2 distinct periods of the year: late fall-winter and spring-late summer

Rasagiline withdrawal Syndrome in Parkinson’s Disease

Parkinson’s disease (PD) patients using dopamine agonists can develop withdrawal symptoms, referred to as dopamine agonist withdrawal syndrome (DAWS), under dose tapering or discontinuation of these drugs. DAWS includes a severe stereotypical cluster of psychiatric and psychological symptoms encompassing severe mood and anxiety disturbances, autonomic symptoms, as well as generalized pain and drug cravings. However, symptoms of withdrawal of dopamine replacement therapies (DRT) are not simply limited to dopamine agonists tapering, as observed in PD patients on deep brain stimulation after dopaminergic drugs withdrawal related to surgery. To date, no DRT-related withdrawal syndrome has been described in PD patients who discontinue rasagiline, an irreversible inhibitor of monoamine oxidase-B (MAO-B). Here we report three PD patients who developed a severe withdrawal syndrome after rasagiline suspension. The syndrome was mainly characterized by prominent psychiatric disorders (depression, anxiety with panic attacks, dysphoria, and agitation) associated with fatigue, generalized pain, and autonomic manifestations (closely resembling symptoms of DAWS). In our opinion, this report suggests the importance of closely monitoring PD patients undergoing rasagiline suspension for withdrawal symptoms and provides interesting points of reflection on the role of rasagiline and other MAO-B inhibitors in mood disorders