O cuidado de enfermagem como prática empreendedora: oportunidades e possibilidades

OBJETIVOS: Compreender o significado do cuidado de enfermagem como prática social empreendedora. MÉTODOS: Foi baseada na Grounded Theory que, de forma sistemática, criativa e interativa possibilitou o desenvolvimento da teoria: "Vislumbrando o cuidado de enfermagem como prática social empreendedora". Amostra teórica constituiu-se de 35 sujeitos entrevistados, distribuídos em diferentes grupos amostrais. RESULTADOS: O cuidado de enfermagem como prática social empreendedora está associado ao sistema de relações e interações, à capacidade de interagir com os diferentes atores sociais, na capacidade de criar novos canais de comunicação e ações pró-ativas. CONCLUSÃO: A partir do cuidado como prática social empreendedora é possível atuar de forma pró-ativa, inovadora e participativa, sem desconsiderar as contradições sociais emergentes.OBJETIVO: Comprender el significado y desarrollar una teoría sustantiva sobre el cuidado de enfermería como una práctica social emprendedora. METODOS: Se basó en la Grounded Theory que, como un proceso sistemático, creativo e interactivo permitió el desarrollo de la teoría sustantiva: "Vislumbrando el cuidado de enfermería como una práctica social emprendedora". La muestra teórica estuvo constituida por treinta y cinco entrevistados distribuidos en diferentes grupos de muestras. RESULTADOS: El cuidado de enfermería como una práctica social emprendedora se asocia con el sistema de relaciones e interacciones, con la capacidad de interactuar con diferentes actores sociales y, con la capacidad de crear nuevos canales de comunicación y de acciones proactivas. CONCLUSIÓN: A partir del cuidado como una práctica emprendedora es posible actuar de manera proactiva, innovadora y participativa, sin dejar de lado las contradicciones sociales emergentes.OBJECTIVE: To understand the meaning and to develop a substantive theory about nursing care as an enterprising social practice. The METHODS: Was based on the Grounded Theory which, in a systematic, creative and interactive manner allowed the development of the substantive theory: "Realizing the nursing care as an enterprising social practice." The theoretical sample consisted of thirty-five subjects distributed among different groups of samples. RESULTS: The nursing care as an enterprising social practice is associated with the system of relationships and interactions, the ability to interact with different social actors and, the ability to create new channels of communication and proactive actions. CONCLUSION: Considering the care as an enterprising practice is possible to act in a proactive, innovative and participatory way, without neglecting the emerging aspects of social contradictions

QSAR-Driven Discovery of Novel Chemical Scaffolds Active against Schistosoma mansoni.

: Schistosomiasis is a neglected tropical disease that affects millions of people worldwide. Thioredoxin glutathione reductase of Schistosoma mansoni (SmTGR) is a validated drug target that plays a crucial role in the redox homeostasis of the parasite. We report the discovery of new chemical scaffolds against S. mansoni using a combi-QSAR approach followed by virtual screening of a commercial database and confirmation of top ranking compounds by in vitro experimental evaluation with automated imaging of schistosomula and adult worms. We constructed 2D and 3D quantitative structure-activity relationship (QSAR) models using a series of oxadiazoles-2-oxides reported in the literature as SmTGR inhibitors and combined the best models in a consensus QSAR model. This model was used for a virtual screening of Hit2Lead set of ChemBridge database and allowed the identification of ten new potential SmTGR inhibitors. Further experimental testing on both shistosomula and adult worms showed that 4-nitro-3,5-bis(1-nitro-1H-pyrazol-4-yl)-1H-pyrazole (LabMol-17) and 3-nitro-4-{[(4-nitro-1,2,5-oxadiazol-3-yl)oxy]methyl}-1,2,5-oxadiazole (LabMol-19), two compounds representing new chemical scaffolds, have high activity in both systems. These compounds will be the subjects for additional testing and, if necessary, modification to serve as new schistosomicidal agents.<br/

Discovery of New Anti-Schistosomal Hits by Integration of QSAR-Based Virtual Screening and High Content Screening.

: Schistosomiasis is a debilitating neglected tropical disease, caused by flatworms of Schistosoma genus. The treatment relies on a single drug, praziquantel (PZQ), making the discovery of new compounds extremely urgent. In this work, we integrated QSAR-based virtual screening (VS) of Schistosoma mansoni thioredoxin glutathione reductase (SmTGR) inhibitors and high content screening (HCS) aiming to discover new antischistosomal agents. Initially, binary QSAR models for inhibition of SmTGR were developed and validated using the Organization for Economic Co-operation and Development (OECD) guidance. Using these models, we prioritized 29 compounds for further testing in two HCS platforms based on image analysis of assay plates. Among them, 2-[2-(3-methyl-4-nitro-5-isoxazolyl)vinyl]pyridine and 2-(benzylsulfonyl)-1,3-benzothiazole, two compounds representing new chemical scaffolds have activity against schistosomula and adult worms at low micromolar concentrations and therefore represent promising antischistosomal hits for further hit-to-lead optimization.<br/