Additional file 7: Table S4. of Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes

Breeding origin, genotype at HvBot1, and B tolerance phenotype of 80 current or recent Australian barley varieties grown to maturity in a glasshouse with an elevated supply of B. Breeding lines Parent 19 and Ethiopia 756 were also included in the screen, as well as Sahara 3771 and Clipper control genotypes. All varieties had a Clipper (B-intolerant) allele at chromosomes 6H and 3H, while at 2H only Sloop Vic_A, Sloop Vic_B and Ethiopia 756 possessed the tolerant (Sahara) allele. Varieties are listed in order of increasing severity of leaf symptoms expression. Symptoms were assessed visually three times during the growth of plants to full maturity and an average score determined (0 = no necrosis; 6 = severe necrosis). The penultimate leaves from five tillers were sampled at mid-grain fill for determination of leaf B concentration (PDF 464 kb

Additional file 5: Table S3. of Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes

Primers, PCR markers and probes used in this study (PDF 350 kb

La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]

19 août 18801880/08/19 (A14,N5217)

Additional file 2: Figure S1. of Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes

Nine barleys accessioned with the name Sahara contain the rare HvBot1 gene duplication. (a) Southern analysis of allele type and gene duplication at HvBot1, for a representative set of barley genotypes. Lane 1, Clipper; 2, Sahara 3771; 3; Alexis; 4, Amagi Nijo; 5, Arapiles; 6, Atlas; 7, Barque; 8, California Mariout; 9, Chebec; 10, CM72; 11, Commander; 12, Flagship; 13, Fleet; 14, Franklin; 15, Gairdner; 16, Galleon; 17, Golden Promise; 18, Halcyon; 19, Haruna Nijo; 20, Clipper; 21, Sahara 3771; 22, Keel; 23, Morex; 24, Mundah; 25, Sahara 3763; 26, Sahara 3764; 27, Sahara 3765; 28, Sahara 3766; 29, Sahara 3767; 30, Sahara 3768; 31, Sahara 3769; 32, Sahara 3770, 33, Schooner; 34, Skiff; 35, Sloop; 36, Steptoe; 37, Tadmor; 38, Tokak. Genomic DNA was digested with Dra I, and probed with a [32P]-labelled nucleic acid fragment of HvBot1 derived from Clipper (Additional File 5: Table S3). (b) Leaf symptom scores (upper panel) and shoot B concentrations (lower panel) of nine Sahara barley accessions grown in nutrient solutions with 5 mM additional B (PDF 1165 kb

Additional file 8: Table S5. of Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes

Barley genotypes included in each (a) HvBot1 allele class and (b) HvNIP2;1 haplotype class, for gene expression analyses data presented in Figs. 1b and 3b, respectively (PDF 301 kb

Baseline demographic characteristics of 1,281 African Americans participating in the Health ABC study.

<p>Definition of abbreviations: BMI  =  body mass index; FEV₁  =  forced expiratory volume at one second; FVC  =  forced vital capacity.</p><p>Categorical and continuous variables were assessed with chi-square test and two-sample t-test, respectively.</p>†<p>among ever smokers only.</p>‡<p>among current smokers only.</p

Longitudinal association between FEV₁ rate of decline, smoking and African ancestry, among African American participants in the Health ABC study.

<p>Mixed effects models are adjusted for sex, standing height, household income, clinic site and two-way interactions among covariates. Age, height and African ancestry are mean centered.</p>†<p>Model 1 is also adjusted for smoking status.</p

Baseline associations between FEV₁ and African ancestry, stratified by smoking status, among African Americans with quality spirometry and no pulmonary disease in the Health ABC study.

<p>Definition of abbreviations: SE  =  standard error; mm  =  millimeter.</p><p>Observations with standardized residuals >2.5 were removed.</p><p><i>P</i> value for interaction for percent African ancestry*smoking status = 0.38, estimated from full model.</p>†<p>African ancestry is coded as a continuous variable.</p

Baseline associations between FEV₁ and smoking, stratified by African ancestry, among smokers with quality spirometry and no pulmonary disease in the Health ABC study.

<p>Definition of abbreviations: SE  =  standard error; mm  =  millimeter.</p><p>Observations with standardized residuals > 2.5 were removed. Median African ancestry is 80.8%, estimated from 1332 ancestry informative markers.</p><p><i>P</i> value for interaction for percent African ancestry*smoking pack-years = 0.17, estimated from full model.</p

Predicted FEV₁ decline (in milliliters) by smoking-ancestry strata derived from a mixed effects model.

<p>Health ABC (A) and CARDIA (B). Current smokers are represented by the ▴ symbol and former smokers by the • symbol. Low African ancestry groups are represented by a solid line and high African ancestry by a dashed line. The groups are as follows: low African current smokers (solid line with ▴ symbol), high African current smokers (dashed line with ▴ symbol), low African former smokers (solid line with • symbol), and high African former smokers (dashed line with • symbol).</p