Additional file 2: Figure S1. of Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes
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Abstract
Nine barleys accessioned with the name Sahara contain the rare HvBot1 gene duplication. (a) Southern analysis of allele type and gene duplication at HvBot1, for a representative set of barley genotypes. Lane 1, Clipper; 2, Sahara 3771; 3; Alexis; 4, Amagi Nijo; 5, Arapiles; 6, Atlas; 7, Barque; 8, California Mariout; 9, Chebec; 10, CM72; 11, Commander; 12, Flagship; 13, Fleet; 14, Franklin; 15, Gairdner; 16, Galleon; 17, Golden Promise; 18, Halcyon; 19, Haruna Nijo; 20, Clipper; 21, Sahara 3771; 22, Keel; 23, Morex; 24, Mundah; 25, Sahara 3763; 26, Sahara 3764; 27, Sahara 3765; 28, Sahara 3766; 29, Sahara 3767; 30, Sahara 3768; 31, Sahara 3769; 32, Sahara 3770, 33, Schooner; 34, Skiff; 35, Sloop; 36, Steptoe; 37, Tadmor; 38, Tokak. Genomic DNA was digested with Dra I, and probed with a [32P]-labelled nucleic acid fragment of HvBot1 derived from Clipper (Additional File 5: Table S3). (b) Leaf symptom scores (upper panel) and shoot B concentrations (lower panel) of nine Sahara barley accessions grown in nutrient solutions with 5Â mM additional B (PDF 1165 kb