Unfreezing water quantity as a possible way of winter wheat frost resistance appreciation

The investigation of unfreezing water quantity change dynamics took place after the plant tissue suffered from the influence of membrane active substances which change water permeability: pipolphen (10-3 M), verapamil (10-4 M), CaCl2 (10-3 M), Gd(NO3)3 (2.5- 10-2 M). The measurements of unfreezing water quantity were made by a simple method on the automatic cogerent «home-made» NMR-plant working at 15 MHz. The objects of investigation were roots of different frost resistant winter wheat (Triticum aestivum) sorts: less frost resistant Mironovskya-808, and more frost resistant Kazanskya-84. The temperature range of unfreezing water quantity was -7° - -30 °C. We got to know that pipolphen and verapamil increase membrane water permeability together with the increase in the quantity of unfreezing water, and the most frost resistant sort Kazanskya-84 had the greatest quantity of unfreezing water in this temperature range. The addition of Ca2+-ions brought about the lowering of unfreezing water. The regularity of influence of the membrane active substances at-30 °C proves that changes in water permeability correlate with those in membrane hydration

Performance of a plastic scintillator developed using styrene monomer polymerization

This paper presents a newly developed plastic scintillator produced in collaboration with Turkiye Energy, Nuclear and Mineral Research Agency (TENMAK). The scintillator is manufactured using thermal polymerization of commercially available styrene monomer. The absorption spectrum of the scintillator exhibited two absorption bands at 225 nm and 340 nm, with an absorption edge observed at 410 nm. The wavelength of the emitted light was measured in the range of 400-800 nm, with a maximum intensity at 427 nm. Monoenergetic electrons from the 137Cs source were used to evaluate the characteristics of the new scintillator, particularly its light yield. As the light readout the MAPD-3NM type silicon photomultiplier array (4 x 4) with an active area of 15 x 15 mm2, assembled using single MAPDs with an active area of 3.7 x 3.7 mm2, was used. The light yield of the scintillator was determined to be 6134 photons/MeV. In addition, the efficiency of the scintillator for gamma rays with an energy of 662 keV was found to be approximately 1.8 %. A CmBe neutron source was employed to evaluate its fast neutron detection performance. However, neutron/gamma discrimination using pulse shape discrimination (charge integration) method was not observed. The results demonstrate the potential of a newly produced plastic scintillator for various applications, particularly in radiation monitoring and detection systems.Comment: 7 pages, 7 figure

Targeting of EGFR by a combination of antibodies mediates unconventional EGFR trafficking and degradation

Antibody combinations targeting cell surface receptors are a new modality of cancer therapy. The trafficking and signalling mechanisms regulated by such therapeutics are not fully understood but could underlie differential tumour responses. We explored EGFR trafficking upon treatment with the antibody combination Sym004 which has shown promise clinically. Sym004 promoted EGFR endocytosis distinctly from EGF: it was asynchronous, not accompanied by canonical signalling events and involved EGFR clustering within detergent-insoluble plasma mebrane-associated tubules. Sym004 induced lysosomal degradation independently of EGFR ubiquitylation but dependent upon Hrs/Tsg101 that are required for the formation of intraluminal vesicles (ILVs) within late endosomes. We propose Sym004 cross-links EGFR physically triggering EGFR endocytosis and incorporation onto ILVs and so Sym004 sensitivity correlates with EGFR numbers available for binding, rather than specific signalling events. Consistently Sym004 efficacy and potentiation of cisplatin responses correlated with EGFR surface expression in head and neck cancer cells. These findings will have implications in understanding the mode of action of this new class of cancer therapeutics

Unfreezing water quantity as a possible way of winter wheat frost resistance appreciation

The investigation of unfreezing water quantity change dynamics took place after the plant tissue suffered from the influence of membrane active substances which change water permeability: pipolphen (10-3 M), verapamil (10-4 M), CaCl2 (10-3 M), Gd(NO3)3 (2.5- 10-2 M). The measurements of unfreezing water quantity were made by a simple method on the automatic cogerent «home-made» NMR-plant working at 15 MHz. The objects of investigation were roots of different frost resistant winter wheat (Triticum aestivum) sorts: less frost resistant Mironovskya-808, and more frost resistant Kazanskya-84. The temperature range of unfreezing water quantity was -7° - -30 °C. We got to know that pipolphen and verapamil increase membrane water permeability together with the increase in the quantity of unfreezing water, and the most frost resistant sort Kazanskya-84 had the greatest quantity of unfreezing water in this temperature range. The addition of Ca2+-ions brought about the lowering of unfreezing water. The regularity of influence of the membrane active substances at-30 °C proves that changes in water permeability correlate with those in membrane hydration

Unfreezing water quantity as a possible way of winter wheat frost resistance appreciation

The investigation of unfreezing water quantity change dynamics took place after the plant tissue suffered from the influence of membrane active substances which change water permeability: pipolphen (10-3 M), verapamil (10-4 M), CaCl2 (10-3 M), Gd(NO3)3 (2.5- 10-2 M). The measurements of unfreezing water quantity were made by a simple method on the automatic cogerent «home-made» NMR-plant working at 15 MHz. The objects of investigation were roots of different frost resistant winter wheat (Triticum aestivum) sorts: less frost resistant Mironovskya-808, and more frost resistant Kazanskya-84. The temperature range of unfreezing water quantity was -7° - -30 °C. We got to know that pipolphen and verapamil increase membrane water permeability together with the increase in the quantity of unfreezing water, and the most frost resistant sort Kazanskya-84 had the greatest quantity of unfreezing water in this temperature range. The addition of Ca2+-ions brought about the lowering of unfreezing water. The regularity of influence of the membrane active substances at-30 °C proves that changes in water permeability correlate with those in membrane hydration

Unfreezing water quantity as a possible way of winter wheat frost resistance appreciation

The investigation of unfreezing water quantity change dynamics took place after the plant tissue suffered from the influence of membrane active substances which change water permeability: pipolphen (10-3 M), verapamil (10-4 M), CaCl2 (10-3 M), Gd(NO3)3 (2.5- 10-2 M). The measurements of unfreezing water quantity were made by a simple method on the automatic cogerent «home-made» NMR-plant working at 15 MHz. The objects of investigation were roots of different frost resistant winter wheat (Triticum aestivum) sorts: less frost resistant Mironovskya-808, and more frost resistant Kazanskya-84. The temperature range of unfreezing water quantity was -7° - -30 °C. We got to know that pipolphen and verapamil increase membrane water permeability together with the increase in the quantity of unfreezing water, and the most frost resistant sort Kazanskya-84 had the greatest quantity of unfreezing water in this temperature range. The addition of Ca2+-ions brought about the lowering of unfreezing water. The regularity of influence of the membrane active substances at-30 °C proves that changes in water permeability correlate with those in membrane hydration