Phytochemical profiles and antioxidant activity of some grape accessions (<i>Vitis</i> spp.) native to Eastern Anatolia of Turkey

Phytochemical profiles and antioxidant activity of four historical grape accessions (‘Kuzu Kuyrugu’, ‘Miskali’, ‘Erkek miskali’, and ‘Kirmizi Kismisi’) grown in Igdir province located in Eastern Anatolia Region of Turkey were examined. Levels of vitamin C, organic acids (citric acid, tartaric acid, malic acid, succinic acid, fumaric acid), sugars (fructose, glucose), phenolic acids (catechin, rutin, quercetin, chlorogenic  acid, ferulic acid, o-coumaric acid, p-coumaric acid, caffeic acid, syringic acid, vanillic acid, and gallic acid), and antioxidant capacity (Trolox Equivalent Antioxidant capacity, TEAC assay) were determined. Accession was found to be important source of variation for all the parameters identified above (P<0.01). Among the grape accessions analyzed, ‘Kuzu Kuyrugu’ had the predominant vitamin C (47.19 mg/100 g), chlorogenic acid (2.687 mg/L), ferulic acid (1.303 mg/L), o-coumaric acid (1.317 mg/L), and syringic acid content (1.687 mg/L). The highest citric acid (55.360 mg/L), fructose (10.36 g/100g), glucose (11.51 g/100g), and catechin (1.353 mg/L) were recorded in ‘Miskali’ genotype. ‘Kirmizi Kismisi’ was determined to be the accession with the highest tartaric acid (21.29 mg/L), succinic acid (0.94 mg/L), and caffeic acid (2.137 mg/L) levels. ‘Erkek Miskali’ accession produced the paramount contents for fumaric acid (0.42 mg/L), rutin (2.477 mg/L), quercetin (0.447 mg/L), and vanillic acid (0.313 mg/L). The investigated grape genotypes showed notable levels of sugars, organic acids and phenolic compounds. These accessions could be valuable in breeding programs for improving grape quality and nutrition, as well as enhancing commercial worth and production of the grapes in Igdir province of Turkey.

An Improved Tissue Culture Medium for Hazelnuts

14th Quadrennial Congress of the International-Association-of-Plant-Biotechnology (IAPB), AUG 19-24, 2018, Dublin, IRELANDWOS: 000440289900158…Int Assoc Plant Biotechnol, Univ Coll Cork, Dow AgroSciences, Environm Protect Agcy, Springer Nature, Teagasc, Wile

Using the CHAID Data Mining Algorithm for Tissue Culture Medium Optimization.

WOS: 000376926800163

MORPHOLOGICAL AND BIOCHEMICAL DIVERSITY IN Rosa SPECIES

The Rosa L. is one of the widely grown plants in the world and its flowers and fruits has been used in different parts of the world for centuries. Wild grown Rosa plants is one of the most important element of the natural landscape and used as rootstock for roses for centuries as well. In this study the main flower, shrub and fruit characteristics of a large number of Rosa canina L. and Rosa dumalis Bechst. genotypes naturally found in Ardahan province of Turkey has been studied. All plants found very health conditions and indicated their strong defense mechanism of the genera to harsh abiotic and biotic conditions. The majority of genotypes had attractive flowers. The genotypes exhibited fruit mass between 2.81 g to 4.60 g. SSC (soluble solid content), vitamin C, total phenolic, total flavonoid, total carotenoid, and total anthocyanin content of the genotypes ranged from 15.8-20.6%, 502-714 mg per 100 g FW (fresh weight), 405-507 mg gallic acid equivalent per 100 g FW, 1.02-2.00 mg per g FW, 8.40-13.30 mg per g FW and 4.35-6.98 mg cyanidin-3-glucoside equivalent per liter, respectively. Antioxidant activity was determined between 22.3-30.9 mg ascorbic acid equivalent per g fresh weight. Chlorogenic acid was the major phenolic acids in fruits of Rosa ecotypes. Our results indicated promising perspectives for usage of R. canina and R. dumalis fresh fruits studied with considerable levels of bioactive compounds

Improved Statistical Analysis for Projecting Mineral Nutrient Ranges

In Vitro Biology Meeting of the Society-for-In-Vitro-Biology (SIVB), JUN 02-06, 2018, St Louis, MOWOS: 000440293300037…Soc In Vitro Bio

Phytochemical Profiles of Wild Grown Blackberry and Mulberry in Turkey

WOS: 000372940400001Blackberry and mulberry are main berry crops grown in Black Sea region mostly in humid shade forest areas and its important for local people's diet and also important for the maintenance of their health and prevention of disease. Present study aimed to determine the content of vitamin C, organic acids (citric acid, tartaric acid, oxalic acid, malic acid, succinic acid, fumaric acid), sugars (glucose and fructose) and phenolic compounds (catechin, rutin, quercetin, chlorogenic acid, ferulic acid, o-coumaric acid, p-coumaric acid, caffeic acid, syringic acid, vanillic acid, and gallic acid), as well as antioxidant capacity (Trolox Equivalent Antioxidant Capacity, TEAC assay) in the wild grown blackberry and mulberry fruit. Among phenolic acids, chlorogenic acid was the predominant for all wild grown blackberries, white and black mulberries. The presence of oxalic acid was only detected in the wild blackberry genotype. As a result, the phytochemical recognition of the wild grown blackberry, black and white mulberries might contribute to forthcoming investigations for developing the efficiency of food industry

Sugars, organic acids, and phenolic compounds of ancient grape cultivars (Vitis vinifera L.) from Igdir province of Eastern Turkey

BACKGROUND: The Eurasian grapevine (Vitis vinifera L.) is the most widely cultivated and economically important horticultural crop in the world. As a one of the origin area, Anatolia played an important role in the diversification and spread of the cultivated form V. vinifera ssp. vinifera cultivars and also the wild form V. vinifera ssp. sylvestris ecotypes. Although several biodiversity studies have been conducted with local cultivars in different regions of Anatolia, no information has been reported so far on the biochemical (organic acids, sugars, phenolic acids, vitamin C) and antioxidant diversity of local historical table V. vinifera cultivars grown in Igdir province. In this work, we studied these traits in nine local table grape cultivars viz. 'Beyaz Kismis' (synonym name of Sultanina or Thompson seedless), 'Askeri', 'El Hakki', 'Kirmizi Kismis', 'Inek Emcegi', 'Hacabas', 'Kerim Gandi', 'Yazen Dayi', and 'Miskali' spread in the Igdir province of Eastern part of Turkey. RESULTS: Variability of all studied parameters is strongly influenced by cultivars (P < 0.01). Among the cultivars investigated, 'Miskali' showed the highest citric acid content (0.959 g/l) while 'Kirmizi Kismis' produced predominant contents in tartaric acid (12.71 g/l). The highest glucose (16.47 g/100 g) and fructose (15.55 g/100 g) contents were provided with 'Beyaz Kismis'. 'Kirmizi Kismis' cultivar had also the highest quercetin (0.55 mg/l), o-coumaric acid (1.90 mg/l), and caffeic acid (2.73 mg/l) content. The highest ferulic acid (0.94 mg/l), and syringic acid (2.00 mg/l) contents were observed with 'Beyaz Kismis' cultivar. The highest antioxidant capacity was obtained as 9.09 μmol TE g-1 from 'Inek Emcegi' in TEAC (Trolox equivalent Antioxidant Capacity) assay. 'Hacabas' cultivar had the highest vitamin C content of 35.74 mg/100 g. CONCLUSIONS: Present results illustrated that the historical table grape cultivars grown in Igdir province of Eastern part of Turkey contained diverse and valuable sugars, organic acids, phenolic acids, Vitamin C values and demonstrated important antioxidant capacity for human health benefits. Further preservation and use of this gene pool will be helpful to avoid genetic erosion and to promote continued agriculture in the region

Field trials in genome edited plants: a bibliometric approach

We have performed bibliometric analysis on field trials of genome edited plants utilizing Scopus and Web of Science core collections. All documents published until May, 2023 were extracted. The final bibliographic data consisted of 39 publications after merging both databases and removing duplicates. Bibliometrix package and Biblioshiny interface available in R studio were utilized to perform the bibliometric analysis. The documents were published in various sources (38) with diverse scopes. Frontiers in Plant Science and Plant Biotechnology Journal were the most active followed by Research Journal of Biotechnology. India and the USA were the leading countries on the topic. The Sankey plot demonstrated that besides publishing on genome editing and field trials, the USA was also publishing in CRISPR/Cas9, acrylamide and biosafety as sub-topics. India was also associated with acrylamide. Acrylamide as a keyword and sub-topic was published in Plant Biotechnology Journal based on our data collection. The most frequent keywords displayed with the word cloud following gene/genome editing and field trials were CRISPR, CRISPR/Cas9, genetic engineering, acrylamide, agronomic traits, biosafety, biotechnology, and crop productivity

Phytochemical profiles of wild blackberries, black and white mulberries from southern Bulgaria

Sugars (glucose and fructose), organic acids (citric acid, tartaric acid, oxalic acid, malic acid, succinic acid and fumaric acid), vitamin C, phenolic compounds (catechin, rutin, quercetin, chlorogenic acid, ferulic acid, o-coumaric acid, p-coumaric acid, caffeic acid, syringic acid, vanillic acid and gallic acid) and antioxidant capacity (Trolox equivalent antioxidant capacity, TEAC assay) were determined in fruits of wild blackberry, black and white mulberries from southern Bulgaria. Malic acid was the predominant organic acid for the black and white mulberries. Citric and malic acids were represented with the highest content in blackberries. The highest fructose concentration was observed in blackberries (16.187 g·100 g−1). Black mulberries showed the highest antioxidant activity (12.230 µmol TE g−1), followed by white mulberries (8.6133 µmol TE g−1) and blackberries (4.490 µmol TE g−1). Chlorogenic acid and rutin were the main phenolics in the analysed fruits. The results illustrated significant phytochemical profiles of the studied berries, which could contribute to the medicinal industry and provide valuable genetic resources for breeding programmes

Genetic diversity detection of seed-propagated walnut (Juglans regia L.) germplasm from Eastern Anatolia using SSR markers

Continuous seed propagation in Turkey has given rise to a great number of seedling walnut trees which represents valuable walnut genetic resources. The number of native walnut trees is estimated to be over 5 million in Turkey and they possess large phenotypic variability in yield, nut and kernel characteristics, late bud breaking, late flowering, winter hardiness and tolerance to diseases. Progress in walnut breeding requires the exploitation of genetic variation among cultivars and landraces. In this study, we used 32 local diverse walnut genotypes obtained from seeds and 2 standard cultivars (‘Sebin’ and ‘Bilecik’). This study implemented 21 previously used simple sequence repeats (SSR) markers to determine genetic diversity. The analysis revealed 135 alleles with an average of 6.43 alleles per locus. Genetic similarity ranged from 0.23 (for samples KW22 and KW29) to 0.87 (for samples KW27 and KW28). The highest number of alleles per locus was obtained from WGA276 locus (11 alleles), followed by WGA054 (9 alleles), WGA202 and WGA321 (8 alleles) while the lowest number was detected in WGA027. According to the morphological and molecular data, the genotypes differed from each other and the cvs. Sebin and Bilecik. The majority of the genotypes had higher fruit weight and some of the genotypes had higher kernel ratio than cvs. Sebin and Bilecik implying the importance of registering genotypes as cultivars. This research provides information on the genetic relationship of walnut genotypes and cultivars and emphasises the importance of protection and utilisation of seed-propagated walnut genetic resources