Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses Against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC50) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.This work was supported by the Russian Science Foundation, Grant No. 20-66-47017

Investigation of cytotoxic effect of new mono- and as-triazines on malignantly degenerated cells

The purpose of the study is to determinate the effectiveness of the antitumor effect of new acridone and xanthone derivatives on tumor cell lines.Цель исследования – определение эффективности противоопухолевого действия исследуемых аналогов акридонов и ксантонов на опухолевые клеточные линии

Development of the method of receiving culture of cells of adenokartsinoma from the metastasis of the breast cancer. Development of model for carrying out laboratory researches for optimization of medical algorithm for maintaining patients

In work the algorithm of receiving culture of cages of a metastasis of a breast cancer for its use as model for carrying out the clinical and safe laboratory researches directed on optimization of medical algorithm for maintaining patients with metastatic damage of a spine column is presented. The received results testify that klonogenny and proliferative activity, and also morphology of the cellular lines allocated from different clones of one tumor differs that allows to assume various induced immunogenicity. The developed algorithm can be used for receiving cellular cultures from tumoral fabrics for creation of an antineoplastic vaccine, researches of an additional expression of genes on the transformed cages, increase of efficiency of the personified therapy for patients of an oncologi profile.В работе представлен алгоритм получения культуры клеток метастаза рака молочной железы для использования ее в качестве модели для проведения клинически-безопасных лабораторных исследований, направленных на оптимизацию лечебного алгоритма для ведения пациентов с метастатическим поражением позвоночного столба. Полученные результаты свидетельствуют, что клоногенная и пролиферативная активность, а также морфология клеточных линий, выделенных из разных клонов одной опухоли отличается, что позволяет предположить различную индуцируемую иммуногенность. Разработанный алгоритм может быть использован для получения клеточных культур из опухолевых тканей для создания противоопухолевой вакцины, исследований добавочной экспрессии генов на трансформированные клетки, повышения эффективности персонифицированной терапии для пациентов онкологического профиля

Acoustic emission during a phase transition of the first kind in a liquid crystal

Crystallization with a volume contraction effect is accompanied by the radiation of sound - acoustic emission (AE). It is proposed in the theory in [2] that the sources of the ultrasonic waves are the fluctuationally occurring nuclei of the daughter phase. The integral characteristics of the AE flux generated in the phase transition are determined by the structure of the nuclei, and the thermokinetics and dynamics of their evolution. However, as a result of methodological errors in the measurement of the AE parameters and the weak dominance over noise of the latter, the available experimental data are obviously insufficient for a unique identification of the mechanisms responsible for the acoustic radiation. The present paper describes the AE which is directly correlated with the elementary processes of the formation and growth of a new phase in the crystallization of a smectic liquid crystal (LC) of cholesterylpelargeonate